Ido Caspy
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idocaspy.bsky.social
Ido Caspy
@idocaspy.bsky.social
95 followers 190 following 0 posts
Postdoc @Bharat group @MRC-LMB
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Reposted by Ido Caspy
tbharat-lab.bsky.social
Assembly, architecture and functional roles of microbial surface layers

Review article published in @natrevmicro.nature.com with @bupbuse.bsky.social, Andriko von Kügelgen and @vikramalva.bsky.social.

S-layers are everywhere!

www.nature.com/articles/s41...
Assembly, architecture and functional roles of microbial surface layers - Nature Reviews Microbiology
Surface layers (S-layers) are ubiquitous protein assemblies that coat prokaryotic cells, with their functional roles increasingly coming into focus. In this Review, Isbilir and colleagues discuss rece...
www.nature.com
November 13, 2025 at 8:14 PM
Reposted by Ido Caspy
tbharat-lab.bsky.social
An Asgard archaeon with internal membrane compartments

Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!

www.biorxiv.org/content/10.1...
November 7, 2025 at 10:44 AM
Reposted by Ido Caspy
tbharat-lab.bsky.social
Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging

Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social

www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social
August 31, 2025 at 6:44 PM
Reposted by Ido Caspy
kashishsingh.bsky.social
Check out our latest work now out on biorxiv www.biorxiv.org/content/10.1...
carter-lab.bsky.social Carter Lab @carter-lab.bsky.social · Aug 5
Excited to share our latest work with @simonbullock11.bsky.social! We looked at how diverse mRNAs get selected for subcellular localization and it turns out that a single protein can recognize different RNA elements using shared features that weren’t apparent before.
www.biorxiv.org/content/10.1...
August 5, 2025 at 11:27 AM
Reposted by Ido Caspy
tbharat-lab.bsky.social
Targeted disruption of phage liquid crystalline droplets abolishes antibiotic tolerance of bacterial biofilms

led by @abultarafder.bsky.social

Great collaboration with @pearce-maths.bsky.social and @geiselbiofilm.bsky.social

www.biorxiv.org/cgi/content/...
July 29, 2025 at 9:35 AM
Reposted by Ido Caspy
kellythd-nguyen.bsky.social
Proud to share our work where we resolved a longstanding question regarding the existence of a human telomerase dimer and provided insights into its function. Led by 3 amazing lab members in collaboration with @yiliangding.bsky.social and @rdaslab.bsky.social.
www.science.org/doi/10.1126/...
Cryo-EM structure of human telomerase dimer reveals H/ACA RNP-mediated dimerization
Telomerase ribonucleoprotein (RNP) synthesizes telomeric repeats at chromosome ends using a telomerase reverse transcriptase (TERT) and a telomerase RNA (hTR in humans). Previous structural work showe...
www.science.org
July 10, 2025 at 9:26 PM
Reposted by Ido Caspy
tbharat-lab.bsky.social
Exciting study from @kiranrpatil.bsky.social and colleagues. Hannah Ochner from the group performed cryo FIB imaging.

Congrats!
mrc-tu.bsky.social MRC Toxicology Unit @mrc-tu.bsky.social · Jul 1
Researchers in the Patil Lab have discovered that certain species of gut bacteria can absorb PFAS. Boosting these species in our gut microbiome could be a new way to protect us from the harmful effects of PFAS.

Read more here: buff.ly/4FsVFsS

@kiranrpatil.bsky.social @indraroux.bsky.social
3D rendering of a bacterium with flagella cut open to show clumps of PFAS molecules inside along with the bacteria's own cellular machinery.
July 1, 2025 at 10:04 AM
Reposted by Ido Caspy
tbharat-lab.bsky.social
Structure of the virulence-associated Neisseria meningitidis filamentous bacteriophage MDAphi

Out in @pnas.org

Congrats @janboehning.bsky.social @abultarafder.bsky.social Miles Graham @mathieucoureuil.bsky.social
@mrclmb.bsky.social @hopitalnecker.bsky.social

www.pnas.org/doi/10.1073/...
June 23, 2025 at 10:26 AM
Reposted by Ido Caspy
tbharat-lab.bsky.social
The mechanics of a continuous self-assembling surface-layer aids cell division in an archaeon

out in @pnas.org

Tight collaboration with Sherman Foo, Alice Cezanne and @buzzbaum.bsky.social, Sulfolobus cryo-ET from @idocaspy.bsky.social

www.pnas.org/doi/10.1073/...
A self-assembling surface layer flattens the cytokinetic furrow to aid cell division in an archaeon | PNAS
The surface layer or “S-layer” is a two-dimensional lattice of proteins that coats a wide range of archaea and bacteria in place of a cell wall or ...
www.pnas.org
June 19, 2025 at 2:06 PM
Reposted by Ido Caspy
tbharat-lab.bsky.social
Finally in peer-reviewed form: Mapping the ultrastructural topology of the corynebacterial cell surface. @bupbuse.bsky.social @vikramalva.bsky.social

Please also see concurrent beautiful work from @adriasogues.bsky.social and Han Remaut.
plosbiology.org PLOS Biology @plosbiology.org · Apr 22
The unique #CellEnvelope of #corynebacteria: @tbharat-lab.bsky.social &co map the C. glutamicum cell surface, revealing a patchy S-layer & specific assembly of the PS2 protein, thereby informing our understanding of cell envelopes that contain #MycolicAcids @plosbiology.org 🧪 plos.io/3Y6JNgw
Left: The atomic model of the PS2 lattice is shown in top and side views. PS2 hexamers are repeated in the 2D sheet with a lattice constant of 176 Å. Each PS2 monomer is colored and labeled separately within the hexamer, which repeats throughout the lattice (with pore dimensions highlighted). The side view of the lattice shows the smooth surface facing the extracellular space and rough surface with coiled-coil segments protruding towards the MM. Top right: Interactions at the hexameric and trimeric interfaces are stabilized by both hydrophilic and hydrophobic residues. Bottom right: A cell envelope tomogram was used to overlay the cryo-EM structure of the S-layer onto the in situ S-layer tomographic density. A single slice of the tomogram is shown, with the overlayed PS2 S-layer lattice in green and the tomogram in grayscale. The S-layer (surface layer), MM (mycomembrane), IM (inner membrane), and Cytosol are labeled.
April 23, 2025 at 2:07 PM
Reposted by Ido Caspy
thibautbrunet.bsky.social
Striking new study from @archaeon-alex.bsky.social's lab just out in @science.org on multicellular development induced by compression in Archaea: www.science.org/doi/10.1126/...
Tissue-like multicellular development triggered by mechanical compression in archaea
The advent of clonal multicellularity is a critical evolutionary milestone, seen often in eukaryotes, rarely in bacteria, and only once in archaea. We show that uniaxial compression induces clonal mul...
www.science.org
April 3, 2025 at 6:24 PM
Reposted by Ido Caspy
tbharat-lab.bsky.social
The mechanics of a continuous self-assembling surface-layer aids cell division in an archaeon

Tight collaboration with @buzzbaum.bsky.social, Sulfolobus cryo-ET from @idocaspy.bsky.social

www.biorxiv.org/content/10.1...
February 6, 2025 at 11:08 AM
Reposted by Ido Caspy
tbharat-lab.bsky.social
Structural biology inside multicellular specimens using electron cryotomography
by @idocaspy.bsky.social @zhexinwang.bsky.social

out now in Quarterly Reviews of Biophysics
doi.org/10.1017/S003...
Structural biology inside multicellular specimens using electron cryotomography | Quarterly Reviews of Biophysics | Cambridge Core
Structural biology inside multicellular specimens using electron cryotomography - Volume 58
doi.org
February 3, 2025 at 1:54 PM
Reposted by Ido Caspy
tbharat-lab.bsky.social
Structure of the Pseudomonas aeruginosa PAO1 Type IV pilus
Finally out in PLOS Pathogens. Congrats Hannah Ochner and co-authors. @mrclmb.bsky.social

journals.plos.org/plospathogen...
December 16, 2024 at 3:53 PM
Reposted by Ido Caspy
biorxiv-cellbio.bsky.social
Tissue-Like Multicellular Development Triggered by Mechanical Compression in Archaea https://www.biorxiv.org/content/10.1101/2024.10.19.619234v1
Tissue-Like Multicellular Development Triggered by Mechanical Compression in Archaea https://www.biorxiv.org/content/10.1101/2024.10.19.619234v1
The advent of clonal multicellularity is a critical evolutionary milestone, seen often in eukaryotes
www.biorxiv.org
October 20, 2024 at 7:30 AM
Reposted by Ido Caspy
archaeon-alex.bsky.social
We have a new story out at BiorXiv! Amazing work led by
Zach Curtis (Bisson), Pedro Escudeiro (Alva) and John Mallon (Bisson). Great collaboration with @vikramalva.bsky.social (Max Planck) and Mecky Pohlschroder (UPenn).

t.co/qGvuQ7mwO1
Halofilins as Emerging Bactofilin Families of Archaeal Cell Shape Plasticity Orchestrators
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
t.co
January 23, 2024 at 5:02 PM