Hugo Brandao
@hugoresearch.bsky.social
biophysics, chromosomes, DNA
This is great, thank you!
December 16, 2024 at 7:00 PM
This is great, thank you!
Fascinating work! Question: from the two example traces, it seems the time intervals become longer with each successive SMC step. Is the rate of extrusion dependent on the DNA torsion? Or, would any slowing down be attributed to the tension (i.e., DNA stretching) imparted by the magnetic tweezers?
December 14, 2024 at 5:46 AM
Fascinating work! Question: from the two example traces, it seems the time intervals become longer with each successive SMC step. Is the rate of extrusion dependent on the DNA torsion? Or, would any slowing down be attributed to the tension (i.e., DNA stretching) imparted by the magnetic tweezers?
Reposted by Hugo Brandao
Today, we report a new discovery about this - a new twist to the story!
These SMC motor proteins do not only extrude the DNA into a loop. While doing so, they also put TWIST into the extruded DNA loop!
See our paper today in Science Advances: www.science.org/doi/10.1126/...
1/
These SMC motor proteins do not only extrude the DNA into a loop. While doing so, they also put TWIST into the extruded DNA loop!
See our paper today in Science Advances: www.science.org/doi/10.1126/...
1/
All eukaryotic SMC proteins induce a twist of −0.6 at each DNA loop extrusion step
Measuring single DNA loop extrusion steps of individual eukaryotic SMCs revealed that ATP binding induces a DNA twist of −0.6.
www.science.org
December 13, 2024 at 7:13 PM
Today, we report a new discovery about this - a new twist to the story!
These SMC motor proteins do not only extrude the DNA into a loop. While doing so, they also put TWIST into the extruded DNA loop!
See our paper today in Science Advances: www.science.org/doi/10.1126/...
1/
These SMC motor proteins do not only extrude the DNA into a loop. While doing so, they also put TWIST into the extruded DNA loop!
See our paper today in Science Advances: www.science.org/doi/10.1126/...
1/