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hughesdc-muscle.bsky.social
Dave Hughes
@hughesdc-muscle.bsky.social
400 followers 43 following 36 posts
Assistant Professor | Skeletal Muscle, Atrophy, Aging, Protein Quality Control (PQC) | Views my own | 📚💪🏼🇬🇧

https://scholar.google.com/citations?user=BuLaLdsAAAAJ&hl=en
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hughesdc-muscle.bsky.social
Dave Hughes @hughesdc-muscle.bsky.social · Oct 18
Played a small part in this project, but there some really interesting and cool findings from the Sharples Lab here. Focus on the memory of repeat muscle atrophy in human skeletal muscle 💪
biorxiv-physio.bsky.social bioRxiv Physiology @biorxiv-physio.bsky.social · Oct 16
Repeated Disuse Atrophy Imprints a Molecular Memory in Skeletal Muscle: Transcriptional Resilience in Young Adults and Susceptibility in Aged Muscle https://www.biorxiv.org/content/10.1101/2025.10.16.681134v1
Reposted by Dave Hughes
profadamsharples.bsky.social
First post on Blue Sky

New pre-print!

Does muscle remember disuse muscle wasting?

Our new study shows skeletal muscle retains a molecular memory of disuse!

Young muscle shows transcriptional resilience to repeated atrophy.

Aged muscle shows exaggerated susceptibility.

doi.org/10.1101/2025...
October 31, 2025 at 4:34 PM
hughesdc-muscle.bsky.social
One of my former mentors now on BlueSky. Welcome @profadamsharples.bsky.social #MyoBlue 💪🏼
October 25, 2025 at 8:48 PM
hughesdc-muscle.bsky.social
Played a small part in this project, but there some really interesting and cool findings from the Sharples Lab here. Focus on the memory of repeat muscle atrophy in human skeletal muscle 💪
biorxiv-physio.bsky.social bioRxiv Physiology @biorxiv-physio.bsky.social · Oct 16
Repeated Disuse Atrophy Imprints a Molecular Memory in Skeletal Muscle: Transcriptional Resilience in Young Adults and Susceptibility in Aged Muscle https://www.biorxiv.org/content/10.1101/2025.10.16.681134v1
October 18, 2025 at 1:15 AM
Reposted by Dave Hughes
unimelbcmr.bsky.social
Checkout the new paper from @hughesdc-muscle.bsky.social & Team, which also involved the CMR's @craigagoodman1.bsky.social
Response of UBR-box E3 ubiquitin ligases & protein quality control pathways to perturbations in protein synthesis & skeletal muscle size
journals.physiology.org/doi/abs/10.1...
Response of UBR-box E3 ubiquitin ligases and protein quality control pathways to perturbations in protein synthesis and skeletal muscle size | American Journal of Physiology-Cell Physiology | American...
The N-degron pathway contributes to proteolysis by targeting N-terminal residues of destabilized proteins via E3 ligases that contain a UBR-box domain. Emerging evidence suggests the UBR-box family of...
journals.physiology.org
October 14, 2025 at 6:35 PM
Reposted by Dave Hughes
craigagoodman1.bsky.social
Congrats to Steffen Raun, Lykke Sylow and Team for their new Redox Biology paper, "Skeletal muscle Rac1 mediates exercise training adaptations towards muscle glycogen resynthesis and protein synthesis", that included the CMR's Prof Paul Gregorevic
www.sciencedirect.com/science/arti...
Skeletal muscle Rac1 mediates exercise training adaptations towards muscle glycogen resynthesis and protein synthesis
Long-term exercise training elicits tremendous health benefits; however, the molecular understanding is incomplete and identifying therapeutic targets…
www.sciencedirect.com
August 30, 2025 at 5:58 AM
hughesdc-muscle.bsky.social
New Preprint alert 🚨 | Future insight into the direction of the lab too #MyoBlue

Response of UBR-box E3 ubiquitin ligases and protein quality control pathways to perturbations in protein synthesis and skeletal muscle size

www.biorxiv.org/content/10.1...
Response of UBR-box E3 ubiquitin ligases and protein quality control pathways to perturbations in protein synthesis and skeletal muscle size
The N-degron pathway contributes to proteolysis by targeting N-terminal residues of destabilized proteins via E3 ligases that contain a UBR-box domain. Emerging evidence suggests the UBR-box family of...
www.biorxiv.org
July 29, 2025 at 1:25 AM
hughesdc-muscle.bsky.social
Applying lessons from limb muscle disuse and ageing to better understand ventilator-induced diaphragm dysfunction | #MyoBlue

pubmed.ncbi.nlm.nih.gov/40638619/
Applying lessons from limb muscle disuse and ageing to better understand ventilator-induced diaphragm dysfunction - PubMed
Mechanical ventilation (MV) is a life-saving intervention applied to critically ill patients. A common consequence of MV is ventilator-induced diaphragm dysfunction (VIDD), which is characterized by s...
pubmed.ncbi.nlm.nih.gov
July 11, 2025 at 6:06 AM
hughesdc-muscle.bsky.social
Muscle AMP deaminase activity was lower in Neandertals than in modern humans

pubmed.ncbi.nlm.nih.gov/40640132/
Muscle AMP deaminase activity was lower in Neandertals than in modern humans - PubMed
The enzyme AMPD1 is expressed in skeletal muscle and is involved in ATP production. All available Neandertal genomes carry a lysine-to-isoleucine substitution at position 287 in AMPD1. This variant, w...
pubmed.ncbi.nlm.nih.gov
July 11, 2025 at 6:00 AM
hughesdc-muscle.bsky.social
Sex differences in resting skeletal muscle and the acute and long-term response to endurance exercise in individuals with overweight and obesity

pubmed.ncbi.nlm.nih.gov/40516819/
Sex differences in resting skeletal muscle and the acute and long-term response to endurance exercise in individuals with overweight and obesity - PubMed
Endurance exercise reduces the risk of metabolic diseases by improving skeletal muscle metabolism, particularly in individuals with overweight and obesity. As biological sex impacts glucose and fatty ...
pubmed.ncbi.nlm.nih.gov
June 15, 2025 at 1:49 PM
hughesdc-muscle.bsky.social
Reduction in Acetylation of Superoxide Dismutase 2 in Skeletal Muscle Improves Exercise Capacity in Mice With Heart Failure | #MyoBlue

pubmed.ncbi.nlm.nih.gov/40511632/
Reduction in Acetylation of Superoxide Dismutase 2 in Skeletal Muscle Improves Exercise Capacity in Mice With Heart Failure - PubMed
The SIRT3 activator Honokiol improved exercise capacity in MI model mice with HF, by improving mitochondrial function in skeletal muscle through the reduction of SOD2 acetylation. SIRT3 activation may...
pubmed.ncbi.nlm.nih.gov
June 14, 2025 at 2:03 AM
hughesdc-muscle.bsky.social
Resistance exercise and skeletal muscle: protein synthesis, degradation, and controversies | #MyoBlue

pubmed.ncbi.nlm.nih.gov/40512179/
Resistance exercise and skeletal muscle: protein synthesis, degradation, and controversies - PubMed
Maintaining and enhancing skeletal muscle mass and strength are essential for optimizing metabolic function and preventing chronic diseases. Resistance exercise plays a pivotal role in this process by...
pubmed.ncbi.nlm.nih.gov
June 14, 2025 at 2:00 AM
Reposted by Dave Hughes
mvfranchi.bsky.social
A new Translational Perspective published in
@jphysiol.bsky.social by Mark Viggars and @kaesser.bsky.social related to our previous manuscript on disuse and retraining

physoc.onlinelibrary.wiley.com/doi/10.1113/...
Reprogramming metabolic gene expression during active recovery following disuse
Click on the article title to read more.
physoc.onlinelibrary.wiley.com
June 1, 2025 at 10:05 AM
hughesdc-muscle.bsky.social
The contribution of age and sex hormones to female neuromuscular function across the adult lifespan | #MyoBlue

pubmed.ncbi.nlm.nih.gov/40349308/
The contribution of age and sex hormones to female neuromuscular function across the adult lifespan - PubMed
Neuromuscular ageing is characterized by neural and/or skeletal muscle degeneration that decreases maximal force and power. Female neuromuscular ageing occurs earlier in life compared to males, potent...
pubmed.ncbi.nlm.nih.gov
May 12, 2025 at 12:50 AM
Reposted by Dave Hughes
craigagoodman1.bsky.social
3D Mitochondrial Structure in Aging Human Skeletal Muscle: Insights Into MFN-2-Mediated Changes
onlinelibrary.wiley.com/doi/10.1111/...
3D Mitochondrial Structure in Aging Human Skeletal Muscle: Insights Into MFN‐2‐Mediated Changes
Changes in mitochondrial structure and dynamics during aging provide a mechanism for the development of age-related sarcopenia, a condition characterized by muscle mass loss. Through the creation of ....
onlinelibrary.wiley.com
April 27, 2025 at 6:02 PM
Reposted by Dave Hughes
craigagoodman1.bsky.social
Muscle mTOR controls iron homeostasis and ferritinophagy via NRF2, HIFs and AKT/PKB signaling pathways
link.springer.com/article/10.1...
Muscle mTOR controls iron homeostasis and ferritinophagy via NRF2, HIFs and AKT/PKB signaling pathways - Cellular and Molecular Life Sciences
Balanced mTOR activity and iron levels are crucial for muscle integrity, with evidence suggesting mTOR regulates cellular iron homeostasis. In this study, we investigated iron metabolism in muscle-spe...
link.springer.com
April 28, 2025 at 5:48 PM
Reposted by Dave Hughes
katsufunai.bsky.social
For those just returning from the APS Summit, I encourage you to submit your work to FUNCTION, an APS journal that covers a wide range of topics relevant to physiology.
Function | Oxford Academic
Function is an online-only, open access journal new in 2020. Function will publish research covering advances in basic, translational, and clinical science that extend the physiological or pathophysio...
academic.oup.com
April 29, 2025 at 1:13 AM
hughesdc-muscle.bsky.social
Muscle wasting in cancer cachexia: Mechanisms and the role of exercise | #MyoBlue

pubmed.ncbi.nlm.nih.gov/40159295/
Muscle wasting in cancer cachexia: Mechanisms and the role of exercise - PubMed
Cancer cachexia (CC) is a multifactorial disease marked by a severe and progressive loss of lean muscle mass and characterized further by inflammation and a negative energy/protein balance, ultimately...
pubmed.ncbi.nlm.nih.gov
April 1, 2025 at 3:08 AM
Reposted by Dave Hughes
craigagoodman1.bsky.social
Mechanotransduction and Skeletal Muscle Atrophy: The Interplay Between Focal Adhesions and Oxidative Stress
www.mdpi.com/1422-0067/26...
www.mdpi.com
March 27, 2025 at 4:51 PM
Reposted by Dave Hughes
rthepple.bsky.social
Attention mitochondriacs! Mitochondrial supercomplexes by cryo-electron tomography. How can you not be blown away by how beautiful this is? Fascinating that ATP synthase is largely organized as dimers since this is one of the putative configurations of mPT pore. www.science.org/doi/10.1126/...
In-cell architecture of the mitochondrial respiratory chain
Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, workin...
www.science.org
March 21, 2025 at 11:44 AM
hughesdc-muscle.bsky.social
The Role and Regulation of Intramuscular Sex Hormones in Skeletal Muscle: A Systematic Review | #MyoBlue

pubmed.ncbi.nlm.nih.gov/40101148/
The Role and Regulation of Intramuscular Sex Hormones in Skeletal Muscle: A Systematic Review - PubMed
Current evidence suggests that ageing and exercise differentially modulate intramuscular sex hormone concentrations, and their association with muscle mass and function, between males and females, and...
pubmed.ncbi.nlm.nih.gov
March 20, 2025 at 1:43 AM
hughesdc-muscle.bsky.social
Increased contact between lipid droplets and mitochondria in skeletal muscles of male elite endurance athletes | #MyoBlue

pubmed.ncbi.nlm.nih.gov/40105858/
Increased contact between lipid droplets and mitochondria in skeletal muscles of male elite endurance athletes - PubMed
Endurance athletes exhibit higher skeletal muscle mitochondrial and lipid droplet (LD) content compared to recreationally active individuals, along with greater whole-body oxygen uptake and maximal fa...
pubmed.ncbi.nlm.nih.gov
March 20, 2025 at 1:42 AM
hughesdc-muscle.bsky.social
Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenance

pubmed.ncbi.nlm.nih.gov/40044687/ | #MyoBlue
Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenance - PubMed
The neuromuscular junction (NMJ) is the synapse formed between motor neurons and skeletal muscle fibers. Its stability relies on the continued expression of genes in a subset of myonuclei, called NMJ ...
pubmed.ncbi.nlm.nih.gov
March 15, 2025 at 5:03 PM
hughesdc-muscle.bsky.social
Mitochondrial fatty acid oxidation regulates adult muscle stem cell function through modulating metabolic flux and protein acetylation

pubmed.ncbi.nlm.nih.gov/40065099/ | #MyoBlue
Mitochondrial fatty acid oxidation regulates adult muscle stem cell function through modulating metabolic flux and protein acetylation - PubMed
During homeostasis and regeneration, satellite cells, the resident stem cells of skeletal muscle, have distinct metabolic requirements for fate transitions between quiescence, proliferation and differ...
pubmed.ncbi.nlm.nih.gov
March 15, 2025 at 5:00 PM
Reposted by Dave Hughes
mattmuscleguy.bsky.social
Congrats to #RueggLab and colleagues on their new #NatComms paper: Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenance. #Myoblue tinyurl.com/yeynb3t2
Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenance - Nature Communications
Here they use single nuclei RNA-seq to identify transcripts in skeletal muscle that maintain the neuromuscular junction, both under normal and denervated conditions, which allows them to characterize ...
tinyurl.com
March 6, 2025 at 12:33 PM
Reposted by Dave Hughes
craigagoodman1.bsky.social
Deubiquitinating Enzymes Regulate Skeletal Muscle Mitochondrial Quality Control and Insulin Sensitivity in Patients With Type 2 Diabetes
onlinelibrary.wiley.com/doi/10.1002/...
Deubiquitinating Enzymes Regulate Skeletal Muscle Mitochondrial Quality Control and Insulin Sensitivity in Patients With Type 2 Diabetes
Background Activation of mitochondrial fission and quality control occur early in the onset of insulin resistance in human skeletal muscle. We hypothesized that differences in mitochondrial dynamics...
onlinelibrary.wiley.com
March 4, 2025 at 5:01 PM