IHBD quantitative 3D image analysis has the potential to advance digital pathology by enabling precise feature extraction, enhancing diagnostic accuracy, and providing deeper insights into tissue architecture. 8/8

We apply this imaging and analysis pipeline to human IHBDs, revealing their complex and heterogeneous 3D architecture. 7/8

We measure features at the whole tissue and individual duct levels to quantify how IHBDs remodel in response to cholestasis (1wk BDL) and hepatocellular injury (4wk thioacetamide). 6/8

To study how IHBDs remodel, we 3D image whole left mouse lobes with invasive and noninvasive ductular reaction (DR), a common phenotype of liver injury. We identify novel morphological changes such as diverticula formation following bile duct ligation (BDL), a cholestasis/noninvasive DR model. 5/8

Our work addresses this gap by providing a robust imaging and analysis pipeline for mouse and human IHBDs. 4/8

This lack of 3D quantitative approaches is particularly limiting for studying tissues with complex 3D organization, such as the IHBDs. Other approaches, such as quantifying conventional histology (2D), can miss subtle or regional phenotypes. 3/8

Access to 3D imaging is rapidly expanding as technologies become more accessible and imaging techniques are increasingly optimized for specific tissues. However, across tissue types, interpretation of whole tissue 3D images is largely qualitative, and standardized analysis workflows are lacking. 2/8

Thank you Adam!

Thank you so much 😄☺️

Thank you so much!

Thank you so much Shashank!

Thank you so much 😄

Would love to be added! I study bile duct morphogenesis in development and regeneration

@hannahhrncir.bsky.social shows that Sox9 is essential for development of small bile ductules in the liver: www.biorxiv.org/content/10.1...