Time for a thread!🧵 How different is the molecular organization of thylakoids in “higher” plants🌱? To find out, we teamed up with @profmattjohnson.bsky.social to dive into spinach chloroplasts with #CryoET ❄️🔬. Curious? ..Read on!

#TeamTomo #PlantScience 🧪 🧶🧬 🌾
elifesciences.org/articles/105...
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Cryo-EM structure of Chlamydomonas Photosystem I complexedwith the alternative electron donor cytochrome c6 www.biorxiv.org/content/10.1101/2025.09.08.674899v1 #cryoem

Our new preprint: how photoprotection is spatially coordinated with energy metabolism. Photosystem I is major source of radicals. We report EM structure of PSI in complex with superoxide dismutase SOD: direct link between energy conversion & oxidative stress defence
www.biorxiv.org/content/10.1...

Let's unpack what our new ferredoxin paper is about, shall we?
✨ She et al., Adv. Sci. (2025) doi.org/10.1002/advs...

We have a preprint for you

EHD2 forms rings on caveolae necks, in contrast to most EHDs forming helices. We determined its structure on membranes and show that N-term acts as a spacer

By Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff &Oli Daumke www.biorxiv.org/content/10.1...

Proud to present the lab's latest work. The full structure of the sodium translocating methyltransferase (Mtr) bound to a small oxygen-responsive small protein MtrI. Work by Tristan and together with @schmitzstreitslab.bsky.social .

www.biorxiv.org/content/10.1101/2025.06.02.657420v1

Ross @milton-group.bsky.social came up with a very similar idea: how about introducing cyanophenylalanine to a (bacterial) ferredoxin that binds two [4Fe-4S] clusters?
One might learn where the electron resides as most ferredoxins are considered one-electron redox mediators... 🧵

Pleased to share my very general review of Photosynthesis now published in Nature Reviews Mol Cell Biol
🌞🌱
rdcu.be/em1lz Hopefully a useful primer for Postgrad and undergrad students

Happy to present the lab latest work, today from the green-side. The ultimate PSII assembly intermediate that directly precedes incorporation of the Mn4O5Ca cluster and formation of the OEC. Psb32 binds to PsbV, challenging the assumption the extrinsic subunits all associate spontaneously in-vivo.

I know there are more important things to worry about right now... but it really pisses me off coming across things like this in the literature.

Nominally 4.8 Å map... check out Fig S1 😬

(also check out any other #cryoem paper by same group, ~all in BBRC)

www.sciencedirect.com/science/arti...

Do you want to join the team? I just got awarded a 2 year fully funded Postdoc position to investigate the metabolism of anaerobic pathogenic bacteria. If you have a strong background in protein biochemistry or anaerobes shown by solid publications, reach out. Job add follows, please repost.

For decades, how methanogenic archaea activate the MCR complex to produce methane remained a mystery. Today in Nature, we report the high-resolution cryo-EM structure of the MCR activation complex!

www.nature.com/articles/s41...

Excited to share our latest work on the structure and mechanism of the ancient sodium-pumping Rnf complex.

www.nature.com/articles/s41...

First bluesky post! About time.

This week, our manuscript revealing the structure, inhibition, and mechanism of human Ceramide Synthase 6 was finally published in NSMB.

We combined cryo-EM and mass spectrometry to reveal its ping-pong mechanism. Check it out!
doi.org/10.1038/s415...

Want to be amazed by how a symbiont can be located at the center of a cell while still being an epibiont! Please read and be amazed by the symbiosis between Anaerameoba and sulfate-reducing bacteria!

Now out in Nature Communications (open access)! rdcu.be/dZGNe
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