Gianluca Pegoraro
Gianluca Pegoraro
@glpego.bsky.social
Reposted by Gianluca Pegoraro
Some closing thoughts for my students this semester on LLMs and learning #rstats datavizf25.classes.andrewheiss.com/news/2025-12...
December 9, 2025 at 8:17 PM
Reposted by Gianluca Pegoraro
some people have security blankets

i have the 40 hidden slides in my presentation just in case i get asked specific obscure questions
December 8, 2025 at 9:58 PM
Reposted by Gianluca Pegoraro
Why do babies need the hepatitis B vaccine if they aren’t high-risk?

Short answer: Because hepatitis B is a tricky virus.

Longer answer: Continue reading ⬇️

🧵1/9 @kkjetelina.bsky.social @meganranney.bsky.social @jasonlschwartz.bsky.social @enirenberg.bsky.social
February 19, 2025 at 2:46 PM
Reposted by Gianluca Pegoraro
If you or your trainees are just getting started with image analysis of cells & are #CellProfiler -curious, here is a fairly thorough walk-through demo I did recently. Loading files, extracting metadata (fun with Regex!), identifying objects, measuring, filtering, QC, exporting:
youtu.be/fO5nJdhj_7I
Introduction to CellProfiler - by a biologist, for biologists!
YouTube video by CellMorphoJSero
youtu.be
December 4, 2025 at 12:37 PM
Reposted by Gianluca Pegoraro
🎄 ANNOUNCEMENT: R Package Development Advent Calendar 2025! 🎄

Starting December 1st, I'm launching a 25-day journey through modern R package development. here's why you should follow along 🧵

#rstats #RPackageAdvent2025 #OpenSource
November 30, 2025 at 10:03 AM
Reposted by Gianluca Pegoraro
So awesome to have this great paper from Sam Reffsin and Sara Cherry out! In it, we use retrospective clone tracing to show that there are particular single cell states that are more susceptible to viral infection (both SARS-CoV-2 and flu)!

www.cell.com/cell/fulltex...
Single-cell susceptibility to viral infection is driven by variable cell states
Not all cells that can be infected by a virus become infected with that virus. Single-cell clone tracing reveals intrinsic cell states with variable expression patterns that increase susceptibility to...
www.cell.com
November 14, 2025 at 4:47 PM
Reposted by Gianluca Pegoraro
Our preprint on scPortrait is out! We built a framework + format to turn microscopy into standardized single-cell image datasets. #scPortrait scales >100M cells, integrates with @scverse.bsky.social, & enables cross-modality modeling from morphology to transcriptomics
doi.org/10.1101/2025...
scPortrait integrates single-cell images into multimodal modeling
Machine learning increasingly uncovers rules of biology directly from data, enabled by large, standardized datasets. Microscopy images provide rich information on cellular architecture and are accessi...
doi.org
September 24, 2025 at 12:22 PM
Reposted by Gianluca Pegoraro
DNA from Napoleon’s 1812 army identifies the pathogens likely responsible for the army’s demise during their Russian retreat. www.cell.com/current-biol...

Nicolás Rascovan & colleagues
@currentbiology.bsky.social
October 24, 2025 at 3:00 PM
Reposted by Gianluca Pegoraro
15 years in the making, we confirmed that mitochondria - the powerhouse of the cell - have an unusual localization in patients who experience psychosis (including schizophrenia and bipolar disorders). You’ll never guess what kind of patient cells we used to make this discovery… 🧵
October 10, 2025 at 4:47 PM
Reposted by Gianluca Pegoraro
Y'ALLLLLLL we got $50k to go to meet our goal! 📈 📈 📈 stjude.org/relay
Relay for St. Jude - St. Jude Children's Research Hospital
Join the Relay community to support the lifesaving mission of St. Jude Children's Research Hospital: Finding cures. Saving children. (registered mark)
stjude.org
September 29, 2025 at 10:40 PM
Reposted by Gianluca Pegoraro
You made me buy it, Darth.

Here's a puppy picture as revenge. Her name is Lily Bug.
August 22, 2025 at 10:04 PM
Reposted by Gianluca Pegoraro
Great news: NIH postbac program is recruiting again! If your grad school plans were affected by program cutbacks or admissions freezes this year I highly encourage you to apply, this could be a perfect opportunity. Please repost.

www.training.nih.gov/research-tra...
www.training.nih.gov
April 10, 2025 at 1:22 AM
Reposted by Gianluca Pegoraro
🎉Congrats to Chase on her new preprint! She developed OMEGA--a simple method for assembling custom gene panels for as little as $1.50 per gene. Big step forward protein engineering and design!🧬
www.biorxiv.org/content/10.1...
Scalable and cost-efficient custom gene library assembly from oligopools
Advances in metagenomics, deep learning, and generative protein design have enabled broad in silico exploration of sequence space, but experimental characterization is still constrained by the cost an...
www.biorxiv.org
March 24, 2025 at 4:50 PM
Reposted by Gianluca Pegoraro
Thanks to Steve Jobs and Gorilla Glass, factories in America are now ramping up to make affordable windows, made from panes thinner than a credit card, that can:

* save Americans $25 billion / year

* withstand a 2x4 shot out of a cannon

(gift link)

www.wsj.com/business/cor...
A Piece of Glass Thinner Than a Credit Card Could Solve America’s $25 Billion Energy Problem
New windows can insulate better than most walls, and some can even survive being hit with a two-by-four shot from a cannon.
www.wsj.com
March 22, 2025 at 2:35 PM
Reposted by Gianluca Pegoraro
One of the remarkable things about #NIH is how rigorously its grants, research, and employees are reviewed. NIH science and scientists are held to a high standard by external reviewers — via study sections and councils, or BSC/site visit reviewers. And the reviews have teeth. 🧪
I’ve served as one of several invited external reviewers for evaluation of an intramural division. The process was rigorous, detailed, and we external reviewers were not afraid to give sharp feedback. Program leadership heard it earnestly; the quality of the intramural science was extraordinary.
February 25, 2025 at 12:26 PM
Reposted by Gianluca Pegoraro
An orphaned wolf pup named Amora has been paired with a rescue puppy named Slinky. Amora needs socialization to develop properly and Slinky is happy to provide it. Sedgwick County Zoo shared that "Slinky’s playful nature is helping her grow into the wolf she is meant to be." 13/10 for both
February 6, 2025 at 12:45 AM
Reposted by Gianluca Pegoraro
40 years ago all of Genbank was published in print form by NAR. The same format today would require over 4 light seconds of shelf space. To a year of progress in 2025.
December 29, 2024 at 9:50 AM
Reposted by Gianluca Pegoraro
Today we report that an engineered skin bacterium, swabbed gently on the head of a mouse, can unleash a potent antibody response against a pathogen. Could lead to topical vaccines that are applied in a cream. @djenetbousbaine.bsky.social led the charge... @natureportfolio.bsky.social 1/55
December 11, 2024 at 4:29 PM
Reposted by Gianluca Pegoraro
A little fun with numbers on this Sunday, showing just how successful DNA really is. First, here's what DNA looks like. I'll draw your attention to the "rise" in DNA, that is, the distance between two basepairs, which is 3.4 Angstroms, also known as 3.4x10^-10 meters...
December 8, 2024 at 9:52 PM