Fantastic write-up on a topic that I have to discuss regularly during microscopy training.

While I just learned of Sanderson’s children’s book, an illustrated book on the ideals of the Knights Radiant could be nice :)

I’m so happy that you both developed and collected such a great variety of LUTs. I think it has allowed for more productive discussion and appreciation of LUTs to occur amongst Fijifolk :)

Also, this is a good opportunity to advertise LUTs like those developed by @fabiocrameri.ch , which are available in the Fiji plugin NeuroCytoLUTs by @christlet.bsky.social

bsky.app/profile/fabi...

Kevin’s plugins below are very helpful to investigate and compare LUTs in Fiji

bsky.app/profile/kwol...

Another aspect to consider is how much colors are saturated, depending on the LUT, the screen type and settings it can make a huge difference on data readability. Green Fire Blue is popular but really saturated

In our case, there was an additional Kozak sequence after the targeting sequence (unintentional, I hope!). We observed cytosolic and mitochondrial targeting, which led us to investigate.

I don't think it's been corrected yet. If you're working with something like mCherry, then there may also be a misfolded non-fluorescent pool that can be visualized by IF (yay 😟). On the positive side, your imaging may get easier if all of the expression is targeted correctly.

Yup, this got our group as well. Interesting error to discover after receiving a plasmid!

Yes, that fixed it. I can open each one and Save as LUT and back into the LUTs folder. They now show up in LUTs manager as anything else would.

They don't. If I open them in Notepad, they (256 rows of 3 8-bit values) don't seem to have the same format as the other .lut files, so that must be the issue, although I can use them in all other applications. As you've made some LUTs, is there an explanation of how to format a .lut file properly?

one more excellent feature: set it up to run on Fiji launch by catching the command in the macro Recorder

Edit -> Options -> Startup
run("Channels and Contrast");
🥳

Hmm, I have a couple of manually added LUTs in my Fiji. Is there a way to get them to be read into the LUT manager also? They are in the luts folder and show up in the Lookup Table menu and in the NeuroCytoLUTs menu (after a few tweaks).

Yes, I was wondering how the estimated description and colors were assigned (and what happens if someone had additional LUTs). Cool, thanks!

one question... how is the LUTs finder annotated/organized?

Very cool features so far! My favorite features : grayscale preview, color groupings, favorite LUTs, merge/split/reorder menus within easy reach, perceptual uniformity scale

Can’t wait to give this a whirl on Monday!