Robert Hänsel-Hertsch
@epistrucstab.bsky.social
Junior Group Leader, Center for Molecular Medicine Cologne, University of Cologne, DE
Image credit: @gloglita.bsky.social @lifescienceeditors.bsky.social captured DynaTag in action: a pA-Tn5 probe (multicoloured) binds an antibody (white), which binds p53 DNA-binding domain (green) on DNA (blue) within 2 nucleosomes
July 28, 2025 at 9:10 AM
Image credit: @gloglita.bsky.social @lifescienceeditors.bsky.social captured DynaTag in action: a pA-Tn5 probe (multicoloured) binds an antibody (white), which binds p53 DNA-binding domain (green) on DNA (blue) within 2 nucleosomes
🗝️ #DynaTag can reveal nuanced TF occupancy behaviour and shed light on transcriptional regulation in health and disease. In small cell lung cancer #SCLC PDX models, it uncovered surprising gain-of-function p53, FOXA1 & MYC activity post-chemotherapy – not for ASCL1, NEUROD1, POU2F3 or YAP1 (5/7)
July 28, 2025 at 9:10 AM
How is DynaTag better than other technologies?
⚡Preserves weak/transient TF binding without signal loss
⚡Better signal-to-noise & resolution
⚡Works for all TFs (high and low DNA-binding affinity) & histone marks
⚡Low-input needed, bulk or single-cell, scalable to multi-cellular systems (4/7)
⚡Preserves weak/transient TF binding without signal loss
⚡Better signal-to-noise & resolution
⚡Works for all TFs (high and low DNA-binding affinity) & histone marks
⚡Low-input needed, bulk or single-cell, scalable to multi-cellular systems (4/7)
July 28, 2025 at 9:10 AM
How is DynaTag better than other technologies?
⚡Preserves weak/transient TF binding without signal loss
⚡Better signal-to-noise & resolution
⚡Works for all TFs (high and low DNA-binding affinity) & histone marks
⚡Low-input needed, bulk or single-cell, scalable to multi-cellular systems (4/7)
⚡Preserves weak/transient TF binding without signal loss
⚡Better signal-to-noise & resolution
⚡Works for all TFs (high and low DNA-binding affinity) & histone marks
⚡Low-input needed, bulk or single-cell, scalable to multi-cellular systems (4/7)
🤔 How to capture nuanced TF-DNA interactions?
💡 Enter #DynaTag: ‘Dynamic targets and Tagmentation’ captures transient TF-DNA interactions that standard CUT&Tag misses.
The trick? Sample prep under physiological salt stabilizes specific interactions without promoting untargeted tagmentation 🧂 (3/7)
💡 Enter #DynaTag: ‘Dynamic targets and Tagmentation’ captures transient TF-DNA interactions that standard CUT&Tag misses.
The trick? Sample prep under physiological salt stabilizes specific interactions without promoting untargeted tagmentation 🧂 (3/7)
July 28, 2025 at 9:10 AM
🤔 How to capture nuanced TF-DNA interactions?
💡 Enter #DynaTag: ‘Dynamic targets and Tagmentation’ captures transient TF-DNA interactions that standard CUT&Tag misses.
The trick? Sample prep under physiological salt stabilizes specific interactions without promoting untargeted tagmentation 🧂 (3/7)
💡 Enter #DynaTag: ‘Dynamic targets and Tagmentation’ captures transient TF-DNA interactions that standard CUT&Tag misses.
The trick? Sample prep under physiological salt stabilizes specific interactions without promoting untargeted tagmentation 🧂 (3/7)
🧪Move over CUT&Tag, there’s a new #TranscriptionFactor mapping method in town.
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)
July 28, 2025 at 9:10 AM
🧪Move over CUT&Tag, there’s a new #TranscriptionFactor mapping method in town.
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)
Our newly developed DynaTag is faster, cleaner, more sensitive than #ChIPseq, #CUT&RUN and #CUT&Tag.
🔗 Our @natcomms.nature.com paper: www.nature.com/articles/s41...
🧵Let’s break down what makes DynaTag so powerful (1/7)
Very proud of Dalya Hiluf being among the poster prize winners at the recent EMBO | EMBL Symposium 'The ageing genome'. Great stuff and congratulations!
June 27, 2025 at 12:44 PM
Very proud of Dalya Hiluf being among the poster prize winners at the recent EMBO | EMBL Symposium 'The ageing genome'. Great stuff and congratulations!