Robert Hänsel-Hertsch
@epistrucstab.bsky.social
Junior Group Leader, Center for Molecular Medicine Cologne, University of Cologne, DE
Incredible work, wow! Massive congratulations to you and your team!!!
August 21, 2025 at 6:50 AM
Incredible work, wow! Massive congratulations to you and your team!!!
Thank you @genomeweb.bsky.social and Andrew P. Han for running a story on DynaTag. www.genomeweb.com/sequencing/b...
You can access the article with an academic/non-profit registration.
You can access the article with an academic/non-profit registration.
Buffer Tweak Opens Up CUT&Tag Chromatin Accessibility Assay to Measure Transcription Factor Binding
The simple reagent swap can enable single-cell and possibly even spatial profiling of transcription factor activity in tissues of many kinds.
www.genomeweb.com
August 15, 2025 at 9:21 PM
Thank you @genomeweb.bsky.social and Andrew P. Han for running a story on DynaTag. www.genomeweb.com/sequencing/b...
You can access the article with an academic/non-profit registration.
You can access the article with an academic/non-profit registration.
Next one is coming up, will contact you soon!
July 29, 2025 at 12:13 PM
Next one is coming up, will contact you soon!
Thanks Karolin, all the credit to Gloria Fuentes @gloglita.bsky.social.
July 28, 2025 at 6:46 PM
Thanks Karolin, all the credit to Gloria Fuentes @gloglita.bsky.social.
Thank you Son for your invaluable help with the skeet! Your scientific illustrations look outstanding!
July 28, 2025 at 11:52 AM
Thank you Son for your invaluable help with the skeet! Your scientific illustrations look outstanding!
Reposted by Robert Hänsel-Hertsch
Die neue Methode übertrifft bestehende Methoden wie #ChIP-seq und #CUT&RUN in Bezug auf Sensitivität und Auflösung der Ergebnisse. 📈
Die Studie wurde in @natcomms.nature.com veröffentlicht👇
www.nature.com/articles/s41...
Die Studie wurde in @natcomms.nature.com veröffentlicht👇
www.nature.com/articles/s41...
DynaTag for efficient mapping of transcription factors in low-input samples and at single-cell resolution - Nature Communications
Transcription factors shape cell identity, but mapping their genomic targets remains challenging. Here the authors present DynaTag, a modified CUT&Tag method for profiling TF occupancy in bulk and...
www.nature.com
July 28, 2025 at 9:45 AM
Die neue Methode übertrifft bestehende Methoden wie #ChIP-seq und #CUT&RUN in Bezug auf Sensitivität und Auflösung der Ergebnisse. 📈
Die Studie wurde in @natcomms.nature.com veröffentlicht👇
www.nature.com/articles/s41...
Die Studie wurde in @natcomms.nature.com veröffentlicht👇
www.nature.com/articles/s41...
Image credit: @gloglita.bsky.social @lifescienceeditors.bsky.social captured DynaTag in action: a pA-Tn5 probe (multicoloured) binds an antibody (white), which binds p53 DNA-binding domain (green) on DNA (blue) within 2 nucleosomes
July 28, 2025 at 9:10 AM
Image credit: @gloglita.bsky.social @lifescienceeditors.bsky.social captured DynaTag in action: a pA-Tn5 probe (multicoloured) binds an antibody (white), which binds p53 DNA-binding domain (green) on DNA (blue) within 2 nucleosomes
Thanks to former PhD student Pascal Hunold and lab members Giulia Pizzolato & Olivia van Ray for their amazing work. Grateful to @dtg-cologne.bsky.social, labs of Peifer, George & Thomas, funders #CMMC @dfg.de #sfb1399 @crc1678.bsky.social #FOR5504 #Fritz_Thyssen_Foundation (7/7)
July 28, 2025 at 9:10 AM
Thanks to former PhD student Pascal Hunold and lab members Giulia Pizzolato & Olivia van Ray for their amazing work. Grateful to @dtg-cologne.bsky.social, labs of Peifer, George & Thomas, funders #CMMC @dfg.de #sfb1399 @crc1678.bsky.social #FOR5504 #Fritz_Thyssen_Foundation (7/7)
The University of Cologne press release can be found here:👇
www.uni-koeln.de/universitaet...
www.uni-koeln.de/en/universit... (6/7)
www.uni-koeln.de/universitaet...
www.uni-koeln.de/en/universit... (6/7)
July 28, 2025 at 9:10 AM
The University of Cologne press release can be found here:👇
www.uni-koeln.de/universitaet...
www.uni-koeln.de/en/universit... (6/7)
www.uni-koeln.de/universitaet...
www.uni-koeln.de/en/universit... (6/7)
🗝️ #DynaTag can reveal nuanced TF occupancy behaviour and shed light on transcriptional regulation in health and disease. In small cell lung cancer #SCLC PDX models, it uncovered surprising gain-of-function p53, FOXA1 & MYC activity post-chemotherapy – not for ASCL1, NEUROD1, POU2F3 or YAP1 (5/7)
July 28, 2025 at 9:10 AM
How is DynaTag better than other technologies?
⚡Preserves weak/transient TF binding without signal loss
⚡Better signal-to-noise & resolution
⚡Works for all TFs (high and low DNA-binding affinity) & histone marks
⚡Low-input needed, bulk or single-cell, scalable to multi-cellular systems (4/7)
⚡Preserves weak/transient TF binding without signal loss
⚡Better signal-to-noise & resolution
⚡Works for all TFs (high and low DNA-binding affinity) & histone marks
⚡Low-input needed, bulk or single-cell, scalable to multi-cellular systems (4/7)
July 28, 2025 at 9:10 AM
How is DynaTag better than other technologies?
⚡Preserves weak/transient TF binding without signal loss
⚡Better signal-to-noise & resolution
⚡Works for all TFs (high and low DNA-binding affinity) & histone marks
⚡Low-input needed, bulk or single-cell, scalable to multi-cellular systems (4/7)
⚡Preserves weak/transient TF binding without signal loss
⚡Better signal-to-noise & resolution
⚡Works for all TFs (high and low DNA-binding affinity) & histone marks
⚡Low-input needed, bulk or single-cell, scalable to multi-cellular systems (4/7)
🤔 How to capture nuanced TF-DNA interactions?
💡 Enter #DynaTag: ‘Dynamic targets and Tagmentation’ captures transient TF-DNA interactions that standard CUT&Tag misses.
The trick? Sample prep under physiological salt stabilizes specific interactions without promoting untargeted tagmentation 🧂 (3/7)
💡 Enter #DynaTag: ‘Dynamic targets and Tagmentation’ captures transient TF-DNA interactions that standard CUT&Tag misses.
The trick? Sample prep under physiological salt stabilizes specific interactions without promoting untargeted tagmentation 🧂 (3/7)
July 28, 2025 at 9:10 AM
🤔 How to capture nuanced TF-DNA interactions?
💡 Enter #DynaTag: ‘Dynamic targets and Tagmentation’ captures transient TF-DNA interactions that standard CUT&Tag misses.
The trick? Sample prep under physiological salt stabilizes specific interactions without promoting untargeted tagmentation 🧂 (3/7)
💡 Enter #DynaTag: ‘Dynamic targets and Tagmentation’ captures transient TF-DNA interactions that standard CUT&Tag misses.
The trick? Sample prep under physiological salt stabilizes specific interactions without promoting untargeted tagmentation 🧂 (3/7)
❗TF occupancy mapping is still challenging. CUT&Tag was a breakthrough, but it has limits…
👉 Doesn’t work well for TFs that only transiently bind DNA
👉 These fleeting interactions are often lost during sample prep
That means we’re missing key regulatory events and it’s time for a new method (2/7)
👉 Doesn’t work well for TFs that only transiently bind DNA
👉 These fleeting interactions are often lost during sample prep
That means we’re missing key regulatory events and it’s time for a new method (2/7)
July 28, 2025 at 9:10 AM
❗TF occupancy mapping is still challenging. CUT&Tag was a breakthrough, but it has limits…
👉 Doesn’t work well for TFs that only transiently bind DNA
👉 These fleeting interactions are often lost during sample prep
That means we’re missing key regulatory events and it’s time for a new method (2/7)
👉 Doesn’t work well for TFs that only transiently bind DNA
👉 These fleeting interactions are often lost during sample prep
That means we’re missing key regulatory events and it’s time for a new method (2/7)