Hope you get some rest! Safe travels the rest of the way!

😆 I made up for it by walking 20k around Copenhagen in one day- and surprisingly, I liked the Little Mermaid!

Haha.. No sword-wielding ceremony, but plenty of delicious Danish pastry

7/ A huge thank you to my incredible team, co-1st authors
Dr Ruofei Liu, PhD student Anthony Xuan and collaborators who made this possible. I can’t wait to see how these insights shape future research! 🙌

6/ Using a small CRISPR-Cas9 synthetic lethal screen with Pol I inhibitors, we uncovered a protective role for RAD54L in nucleolar DDR. Loss of RAD54L increases R-loops and rDNA damage, disrupting nucleolar structure and sensitizing cells to PARP and RNA Pol I inhibitors. 🧬🛠️

5/ We uncovered that upon rDNA damage, nucleolar Pol II binds to intergenic rDNA sequences, generating R-loops. These R-loops recruit DDR factors, playing a pivotal role in the early response to rDNA damage and the nucleolar DDR response. 🧬✨

4/ We identified early changes in nucleolar-nucleoplasmic proteome localisation upon rDNA damage and uncovered replication stress signatures involving chromatin remodelling, transcription-replication conflicts, and DNA repair pathways. 🌀

3/ In this study, we profiled the nucleolar proteome and chromatin landscape of stalled replication sites upon rDNA damage. This allowed us to identify the early steps of nucleolar DDR and provided a valuable resource for investigating the response to replication stress at rDNA.

2/ rRNA genes are transcribed by up to 150 RNA Pol I enzymes simultaneously, creating a challenge for replication machinery. The rDNA loci are hotspots for DNA damage, but the dynamics of repair in these regions are not fully understood. 🧬⚡️

Congrats to you and the team on a great year! 👏

Congrats Irene 🎉 I’m glad we managed a quick celebration! Here’s to many more great papers and achievements 🥂