Dan Depledge
@dandepledge.bsky.social
Associate Professor @ Hannover Medical School.
Virology, RNA biology, and Computational Biology.
Global villager.
Lab website: http://depledgelab.github.io
Virology, RNA biology, and Computational Biology.
Global villager.
Lab website: http://depledgelab.github.io
Our latest preprint is online!
In which we take a left turn and dive head first into the biology of the RNA polymerase III transcriptome and epitranscriptome
Do you want to know more? A small thread follows👇
www.biorxiv.org/content/10.1...
In which we take a left turn and dive head first into the biology of the RNA polymerase III transcriptome and epitranscriptome
Do you want to know more? A small thread follows👇
www.biorxiv.org/content/10.1...
Defining expansions and perturbations to the RNA polymerase III transcriptome and epitranscriptome by modified direct RNA nanopore sequencing
RNA polymerase III (Pol III) transcribes cytosolic transfer RNAs (tRNAs) and other non-coding RNAs (ncRNAs) essential to cellular function. However, many aspects of Pol III transcription and processin...
www.biorxiv.org
March 14, 2025 at 12:03 PM
Our latest preprint is online!
In which we take a left turn and dive head first into the biology of the RNA polymerase III transcriptome and epitranscriptome
Do you want to know more? A small thread follows👇
www.biorxiv.org/content/10.1...
In which we take a left turn and dive head first into the biology of the RNA polymerase III transcriptome and epitranscriptome
Do you want to know more? A small thread follows👇
www.biorxiv.org/content/10.1...
Reposted by Dan Depledge
A teaser for our new preprint that will be available soon!!!
Pol III, RNA modifications, tRNAs, viruses, and of course, nanopores
Pol III, RNA modifications, tRNAs, viruses, and of course, nanopores
March 7, 2025 at 11:32 AM
A teaser for our new preprint that will be available soon!!!
Pol III, RNA modifications, tRNAs, viruses, and of course, nanopores
Pol III, RNA modifications, tRNAs, viruses, and of course, nanopores
Reposted by Dan Depledge
The master’s program in #biomedical_data_science, which was developed as part of RESIST, has now graduated for the second time: 18 students from the first two years successfully completed their studies. www.resist-cluster.de/en/festive-s... 🎉 @dandepledge.bsky.social @bosse-lab.bsky.social
Festive setting
Festive setting for the presentation of certificates to students of biomedical data science: The master's program in biomedical data science, which was developed as part of the RESIST project, has now...
www.resist-cluster.de
February 12, 2025 at 9:15 AM
The master’s program in #biomedical_data_science, which was developed as part of RESIST, has now graduated for the second time: 18 students from the first two years successfully completed their studies. www.resist-cluster.de/en/festive-s... 🎉 @dandepledge.bsky.social @bosse-lab.bsky.social
Reposted by Dan Depledge
Happy to share our publication in #PlosPathogens investigating repression of varicella zoster virus in neuron-like cells: journals.plos.org/plospathogen...
Thanks to @dandepledge.bsky.social and other colleagues for their contribution!
Thanks to @dandepledge.bsky.social and other colleagues for their contribution!
Repression of varicella zoster virus gene expression during quiescent infection in the absence of detectable histone deposition
Author summary Varicella zoster virus (VZV) establishes latent infection exclusively in human neurons. This phase of infection is characterized by restricted viral gene expression, lack of detectable ...
journals.plos.org
February 20, 2025 at 5:26 PM
Happy to share our publication in #PlosPathogens investigating repression of varicella zoster virus in neuron-like cells: journals.plos.org/plospathogen...
Thanks to @dandepledge.bsky.social and other colleagues for their contribution!
Thanks to @dandepledge.bsky.social and other colleagues for their contribution!
Congratulations to all the graduates of the MHH Biomedical Data Science Masters programme! The graduation ceremony today was a great success and we really appreciated @bosse-lab.bsky.social giving a great lecture!
February 6, 2025 at 7:30 PM
Congratulations to all the graduates of the MHH Biomedical Data Science Masters programme! The graduation ceremony today was a great success and we really appreciated @bosse-lab.bsky.social giving a great lecture!
Love it when you read a review that completely unlocks a new field of biology that you are working in.
January 29, 2025 at 11:25 AM
Love it when you read a review that completely unlocks a new field of biology that you are working in.
me: i need help with some visa questions
local admin directly responsible for such matters: no
me: ???
admin: you need to sort this out yourself
admin: also, you are not properly registered as a foreigner in our system, this is clearly your fault and definitely not ours...
me: ???
local admin directly responsible for such matters: no
me: ???
admin: you need to sort this out yourself
admin: also, you are not properly registered as a foreigner in our system, this is clearly your fault and definitely not ours...
me: ???
January 8, 2025 at 8:30 AM
me: i need help with some visa questions
local admin directly responsible for such matters: no
me: ???
admin: you need to sort this out yourself
admin: also, you are not properly registered as a foreigner in our system, this is clearly your fault and definitely not ours...
me: ???
local admin directly responsible for such matters: no
me: ???
admin: you need to sort this out yourself
admin: also, you are not properly registered as a foreigner in our system, this is clearly your fault and definitely not ours...
me: ???
First day back at work and so far I have a broken computer, a VPN that cannot maintain it's connection and half my lab has been lost to a time management course that, no kidding, is 8 hours long!!!
today has been cancelled go back to bed
ALT: today has been cancelled go back to bed
media.tenor.com
January 7, 2025 at 9:18 AM
First day back at work and so far I have a broken computer, a VPN that cannot maintain it's connection and half my lab has been lost to a time management course that, no kidding, is 8 hours long!!!
Thank goodness for section 81 paragraph 4 of the German residence act!
January 3, 2025 at 4:13 PM
Thank goodness for section 81 paragraph 4 of the German residence act!
2025 goals
Get off facebook - done
Get off twitter/x - done
Navigate German bureaucracy to obtain a settlement permit so that I am not forced to leave the EU in June - pending
Brexit, truly the gift that keeps on giving...
Get off facebook - done
Get off twitter/x - done
Navigate German bureaucracy to obtain a settlement permit so that I am not forced to leave the EU in June - pending
Brexit, truly the gift that keeps on giving...
January 2, 2025 at 6:53 PM
2025 goals
Get off facebook - done
Get off twitter/x - done
Navigate German bureaucracy to obtain a settlement permit so that I am not forced to leave the EU in June - pending
Brexit, truly the gift that keeps on giving...
Get off facebook - done
Get off twitter/x - done
Navigate German bureaucracy to obtain a settlement permit so that I am not forced to leave the EU in June - pending
Brexit, truly the gift that keeps on giving...
Making progress...
December 2, 2024 at 7:18 PM
Making progress...
So good to see membership of this platform increasing so fast. So much great science content already :)
November 17, 2024 at 12:27 PM
So good to see membership of this platform increasing so fast. So much great science content already :)
Nice to see the communities of RNA biologists and herpesvirologists are growing on here. Now we just need more nanopore people!
November 11, 2024 at 8:09 AM
Nice to see the communities of RNA biologists and herpesvirologists are growing on here. Now we just need more nanopore people!
Remind me again why I left the wonderful sun and blue skies of NYC...
November 13, 2023 at 6:23 AM
Remind me again why I left the wonderful sun and blue skies of NYC...
Reposted by Dan Depledge
The HZI will hire up to twenty (20!) tenure-track research groups under the broad topic of "Microbial Stargazing" (🦠🌌). The first round of 4 positions is now open; the call closes on the 10th of December. Please share! www.helmholtz-hzi.de/en/career/jo...
November 6, 2023 at 12:52 PM
The HZI will hire up to twenty (20!) tenure-track research groups under the broad topic of "Microbial Stargazing" (🦠🌌). The first round of 4 positions is now open; the call closes on the 10th of December. Please share! www.helmholtz-hzi.de/en/career/jo...
Trying to write computational/sequencing focused paper that explores the biology of three very different viruses is proving a bigger challenge than I had anticipated...
November 2, 2023 at 9:01 AM
Trying to write computational/sequencing focused paper that explores the biology of three very different viruses is proving a bigger challenge than I had anticipated...
Great to see this online and very happy to have contributed to this study led by Hannah Burgess (NYU & University of Surrey) and Ian Mohr (NYU)!!!
www.embopress.org/doi/full/10....
#is_there_anything_nanopores_cant_do
www.embopress.org/doi/full/10....
#is_there_anything_nanopores_cant_do
EMBO Press is an editorially independent publishing platform for the development of EMBO scientific publications.
www.embopress.org
October 17, 2023 at 1:27 PM
Great to see this online and very happy to have contributed to this study led by Hannah Burgess (NYU & University of Surrey) and Ian Mohr (NYU)!!!
www.embopress.org/doi/full/10....
#is_there_anything_nanopores_cant_do
www.embopress.org/doi/full/10....
#is_there_anything_nanopores_cant_do
Very much looking forward to reading this, especially with coronaviruses in mind as a number of studies (inc one I contributed to) have reported varying levels of m6A to be present (validated by orthogonal methods) which remains counterintuitive in many ways...
N6-methyladenosine modification is not a general trait of viral RNA genomes https://www.biorxiv.org/content/10.1101/2023.10.13.561839v1
N6-methyladenosine modification is not a general trait of viral RNA genomes https://www.biorxiv.org/content/10.1101/2023.10.13.561839v1
Despite the nuclear localization of the m6A machinery, the genomes of multiple exclusively-cytoplasm
www.biorxiv.org
October 14, 2023 at 4:46 PM
Very much looking forward to reading this, especially with coronaviruses in mind as a number of studies (inc one I contributed to) have reported varying levels of m6A to be present (validated by orthogonal methods) which remains counterintuitive in many ways...
Reposted by Dan Depledge
The lab has just been awarded an 8-year grant to study coronavirus replication! 🍾🎉
Job ads will appear around end October but we are particularly looking for PDRAs with coronavirus reverse genetics experience or CL3 coronavirus-host interactions. ~Mar 2024 start.
Job ads will appear around end October but we are particularly looking for PDRAs with coronavirus reverse genetics experience or CL3 coronavirus-host interactions. ~Mar 2024 start.
September 27, 2023 at 9:37 AM
The lab has just been awarded an 8-year grant to study coronavirus replication! 🍾🎉
Job ads will appear around end October but we are particularly looking for PDRAs with coronavirus reverse genetics experience or CL3 coronavirus-host interactions. ~Mar 2024 start.
Job ads will appear around end October but we are particularly looking for PDRAs with coronavirus reverse genetics experience or CL3 coronavirus-host interactions. ~Mar 2024 start.
Still recovering from the mini herpesvirus conference (+ assorted bugs the kids brought home from daycare) but still happily reflecting on what an interesting and fun day it was!!!
Lots of thanks to go around but I would particularly highlight our two plenary speakers and our army of volunteers!
Lots of thanks to go around but I would particularly highlight our two plenary speakers and our army of volunteers!
October 1, 2023 at 12:08 PM
Still recovering from the mini herpesvirus conference (+ assorted bugs the kids brought home from daycare) but still happily reflecting on what an interesting and fun day it was!!!
Lots of thanks to go around but I would particularly highlight our two plenary speakers and our army of volunteers!
Lots of thanks to go around but I would particularly highlight our two plenary speakers and our army of volunteers!
The 16th mini herpesvirus workshop is in full swing! Over 100 participants from 33 institutions across six countries.
An excellent plenary talk from Hannah Burgess (University of Surrey) on the role of the CCR4-NOT complex in HCMV infections (that has just been accepted for publication)!
An excellent plenary talk from Hannah Burgess (University of Surrey) on the role of the CCR4-NOT complex in HCMV infections (that has just been accepted for publication)!
September 29, 2023 at 12:43 PM
The 16th mini herpesvirus workshop is in full swing! Over 100 participants from 33 institutions across six countries.
An excellent plenary talk from Hannah Burgess (University of Surrey) on the role of the CCR4-NOT complex in HCMV infections (that has just been accepted for publication)!
An excellent plenary talk from Hannah Burgess (University of Surrey) on the role of the CCR4-NOT complex in HCMV infections (that has just been accepted for publication)!
Easily one of my favorite books ever!
Fuck me, TIME WAR. This is how *I* lose the tome war JFC
THIS IS HOW YOU LOSE THE TOME WAR. A wonder of a book. Almost unbelievable that it’s the work of two writers, it feels so coherent. A deliciously old-fashioned idea—pen friends fall in love!—clothed in boundless creativity.
September 27, 2023 at 3:56 PM
Easily one of my favorite books ever!
Two days until the 16th mini herpesvirus workshop!
September 27, 2023 at 3:28 PM
Two days until the 16th mini herpesvirus workshop!
Reposted by Dan Depledge
m7GpppNm - VERY EXCITED to share my postdoctoral work in @ramlabuw.bsky.social’s lab, now @bioRxiv. We find that cellular RNA interacts with MAVS, the adaptor protein of the RIG-I-like receptor pathway, to promote antiviral signaling. A 🧵:
Cellular RNA interacts with MAVS to promote antiviral signaling https://www.biorxiv.org/content/10.1101/2023.09.25.559083v1
Cellular RNA interacts with MAVS to promote antiviral signaling https://www.biorxiv.org/content/10.1101/2023.09.25.559083v1
Immune signaling needs to be well-regulated to promote clearance of pathogens, while preventing aber
www.biorxiv.org
September 26, 2023 at 8:07 AM
m7GpppNm - VERY EXCITED to share my postdoctoral work in @ramlabuw.bsky.social’s lab, now @bioRxiv. We find that cellular RNA interacts with MAVS, the adaptor protein of the RIG-I-like receptor pathway, to promote antiviral signaling. A 🧵: