This workflow is very nice for organoids, where you want to take your time to pick and choose which organoids you re-image at sub-cellular resolution.

The team published LUMI v2.0! The best new feature defines ROIs on low-res 3D scout scans!

Scan your whole tissue at low resolution

Define a bounding box using something like BigStitcher

Import and scan the ROI

Take your time finding and defining an ROI without worrying about photobleaching!

I hope I never get tired of how amazing cells look in 3D. Happy Holidays and Happy #FluorescenceFriday! Here are CD45+ cells in a skin biopsy. The segmented cells and nerves at the end of the video kind of look like strings of lights

For #fluorescencefriday here is a mouse brain stained with Eosin and To-PRO-3 showing a segmented Glioblastoma in yellow. While this whole brain computational H&E staining is cool, I think I prefer TH staining for the dendrites. Imaged on Alpenglow Bioscience's 3Di #light-sheet #microscopy

From Maple Leaf

Very cool work! I really like the videos showing off the segmentation

Here is a murine heart video we imaged with the Red-Horse lab for #FluorescenceFriday.

There are some really cool supplemental videos

This is the best one I've found this fall

Lets start this off with some 3D imaging of human placenta. Samples from Mira Moufarrej and the lab of Kristy Red Horse at Stanford. Imaged at Alpenglow Biosciences in Seattle by our amazing team of scientists and engineers.