Cris
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cristn.bsky.social
Cris
@cristn.bsky.social
110 followers 270 following 26 posts
Interesses nessa rede: Covid, Política, Ciências, Direito, Mudanças Climáticas e um pouco de Humor também.
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Reposted by Cris
sailorrooscout.bsky.social
against not just the original SARS-CoV-2 virus, but ALL variants of SARS-CoV-2 known to cause illness in humans. When tested in mouse models, the antibodies also SIGNIFICANTLY reduced the viral load in the lungs of mice exposed to Omicron variants.
Fig. 5. Deep mutational scanning of the SARS-CoV-2 XBB.1.5 spike protein for the C952-C1596 antibody cocktail or CoV2-biRN5 bsAb shows potential escape mutations. (A) The line plot shows the sum of escape for each amino acid residue in the SARS-CoV-2 XBB.1.5 S1 subunit (residues 1 to 600) as determined by a pseudovirus deep mutational scan with an equimolar cocktail of C952 and C1596 IgGs (top) or CoV2-biRN5 bsAb (bottom). Epitope residues for C952 and C1596 are highlighted with orange and purple shading, respectively. (B) BLI association and dissociation curves are shown, illustrating the effect of escape mutations in the SARS-CoV-2 XBB.1.5 S 6P on C1596 Fab binding. The concentrations of C1596 tested are indicated in each panel. The dissociation phase begins at time = 150 s and is depicted by a dotted vertical line. Fit curves are depicted as black solid lines. (C) Surface rendering of SARS-CoV-2 XBB.1.5 spike protein colored by the sum of escape scores, which is calculated as the sum of escape for all mutations at each site. Key residues of escape labeled along with their frequency across 5,240,975 sequences uploaded to GISAID CoVsurver as of 23 October 2024; frequency is denoted for the escape mutation with the greatest escape score (upper value) and for all amino acid mutations at that residue site (lower value). Fig. 6. Bispecific CoV2-biRN5 demonstrates prophylactic efficacy in a K18-hACE2 mouse model. (A) Shown is a schematic representation of the experimental timeline for assessing the efficacy of CoV2-biRN5 in vivo. K18-hACE2 mice received 5 mg kg−1 of either the cocktail of C952 and C1596 IgGs or the CoV2-biRN5 bsAb by intraperitoneal (ip) injection 24 hours before or 4 hours after intranasal (i.n.) inoculation with 2 × 104 PFU of the SARS-CoV-2 XBB.1.5 virus. Lungs were harvested at 3 days after infection. (B and C) Viral RNA load of SARS-CoV-2 XBB.1.5 in the lung was measured for each group (line indicates mean; n = 9 for untreated control and 5 for all other treatment groups) in the prophylaxis (B) and treatment (C) arms. Data were analyzed using one-way ANOVA, correcting for multiple comparisons with Dunnett’s test with comparison with the untreated control. ns, not significant, **P < 0.01, *P < 0.05. The dotted line indicates the lower limit of detection of the assay. The bispecific antibody’s design was specifically created to overcome challenges presented by SARS-CoV-2’s mutations. According to researchers, the antibody C1596, which targets the NTD, binds to a quaternary epitope, a region of the virus’s spike protein that doesn’t change frequently. This antibody, when paired with C952, an antibody targeting the RBD, resulted in increased neutralization efficacy in laboratory experiments, particularly against variants that have emerged after Omicron, such as XBB.1.5. In the study, the researchers found that the antibody pair was most effective when C1596 was placed at the N-terminal position, reinforcing the idea that the order in which the antibodies are combined matters for achieving the highest possible neutralization activity. This discovery will guide future research to further optimize bispecific antibodies for both efficacy and manufacturability.
March 9, 2025 at 4:09 PM
Reposted by Cris
sailorrooscout.bsky.social
The study has been published in Science. YES, it is PEER-REVIEWED.
• www.science.org/doi/10.1126/...
Abstract The ongoing emergence of severe acute respiratory syndrome coronavirus (SARS-CoV-2) variants of concern (VOCs) that reduce the effectiveness of antibody therapeutics necessitates development of next-generation antibody modalities that are resilient to viral evolution. Here, we characterized amino-terminal domain (NTD)- and receptor binding domain (RBD) -specific monoclonal antibodies previously isolated from coronavirus disease 2019 (COVID-19) convalescent donors for their activity against emergent SARS-CoV-2 VOCs. Among these, the NTD-specific antibody C1596 displayed the greatest breadth of binding to VOCs, with cryo-electron microscopy structural analysis revealing recognition of a distinct NTD epitope outside of the site i antigenic supersite. Given C1596's favorable binding profile, we designed a series of bispecific antibodies (bsAbs), termed CoV2-biRNs, that featured both NTD and RBD specificities. Two of the C1596-inclusive bsAbs, CoV2-biRN5 and CoV2-biRN7, retained potent in vitro neutralization activity against all Omicron variants tested, including XBB.1.5, BA.2.86, and JN.1, contrasting the diminished potency of parental antibodies delivered as monotherapies or as a cocktail. Furthermore, prophylactic delivery of CoV2-biRN5 reduced the viral load within the lungs of K18-hACE2 mice after challenge with SARS-CoV-2 XBB.1.5. In conclusion, NTD-RBD bAbs offer promising potential for the design of resilient, next-generation antibody therapeutics against SARS-CoV-2 VOCs. An illustration of the bispecific antibodies the Stanford-led research team developed to neutralize the virus that causes COVID-19. Named “CoV2-biRN,” these two antibodies work together by attaching to different areas of the virus. Credit: Christopher O. Barnes/Adonis Rubi The bispecific antibodies target two areas of the virus: One attaches to the “NTD,” or Spike N-terminal domain, an area on the virus that does not change very much. This allows the second antibody to attach to the “RBD,” or receptor-binding domain, essentially preventing the virus from infecting human cells. Credit: Christopher O. Barnes/Adonis Rubio
March 9, 2025 at 4:01 PM
Reposted by Cris
flabberghaster.bsky.social
There are plenty of studies saying that yes, it might cause immune system dysregulation. Here are some random examples:

- www.nature.com/articles/s41...
- www.nih.gov/news-events/...

I don't get why there's such a push to say "no, getting covid is fine, don't worry about it" from so many.
Long COVID manifests with T cell dysregulation, inflammation and an uncoordinated adaptive immune response to SARS-CoV-2 - Nature Immunology
Roan et al. use Olink and single‐cell RNA sequencing (scRNA-seq) to show a dysregulated crosstalk between the cellular and humoral immune responses in individuals with long COVID 8 months postinfectio...
www.nature.com
September 21, 2024 at 11:01 PM
Reposted by Cris
mellziland.bsky.social
Dentro desse contexto das vacinas atualizadas, há uma movimentação da sociedade civil para levar essa demanda (da urgência da aquisição e disponibilidade) de vacinas atualizadas da Covid-19 para o @minsaude.bsky.social

Vou deixar o link abaixo:
Vacina atualizada contra covid19 nas versões para as variantes KP2 e JN1
Ao Excelentíssimo Sr. Luiz Inácio Lula da Silva, Presidente da RepúblicaÀ Excelentíssima Sra. Nísia Trindade Lima, Ministra da Saúde do BrasilAo Excelentíssimo Sr. Alexandre Padilha, Ministro das Rela...
secure.avaaz.org
September 19, 2024 at 2:30 PM
cristn.bsky.social
Seria pra usar por curto período, quando tiver q sair pros locais com muita fumaça. No restante do tempo uso a PFF2
September 16, 2024 at 10:24 PM
cristn.bsky.social
Qualidade do ar da escola 115 Norte nesse momento:
September 16, 2024 at 10:54 AM
cristn.bsky.social
Bruno, eu queria comprar uma máscara elastomérica, mas não sei escolher o filtro. Achei essas opções abaixo. Qual é a melhor pra proteger dos gases tóxicos da fumaça?
September 16, 2024 at 10:51 AM
cristn.bsky.social
E desde ontem à noite o Parque Nacional de Brasília está pegando fogo. Qualidade do ar nesse momento na Asa Norte é essa aqui:
September 16, 2024 at 10:50 AM
cristn.bsky.social
A @biaklimeck tá aqui no Bsky tb trazendo essas orientações.
September 16, 2024 at 9:59 AM
cristn.bsky.social
Recomende q as pessoas evitem exposição e usem máscaras PFF2, q podem ser compradas em lojas de materiais de construção, Brunno: www.instagram.com/p/C_4ObKnv6E...
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September 16, 2024 at 9:57 AM
cristn.bsky.social
Contra. Quem tem criança com dificuldades de sono sofre pra fazer a adaptação e depois a readaptação.
September 12, 2024 at 9:07 AM
cristn.bsky.social
O MS precisa fazer campanhas massivas explicando como a covid ainda pode ser grave, q há Covid Longa, q vacinas são seguras e efeitos adversos existem em qualquer remedinho, precisam comprar vacinas e incentivar a vacinação p/ covid-19. Não é e nunca foi uma gripezinha.
September 11, 2024 at 9:22 PM
cristn.bsky.social
E se a pessoa puder comprar uma máscara mais cara, tipo uma de entrar num imóvel pegando fogo (se existir), qual filtro teria que comprar?
September 11, 2024 at 10:05 AM