B: integrate, then cluster and annotate and then do sample specific sub-clustering

would love to have your input or ideas

At the end it is reflecting what we value as a society which is the output regardless of understanding
with big data and rapidly evolving world we do need AI assistance but maybe redirected towards gaining skills and knowledge rather than accepted final output

Hi @erikmcintire.bsky.social can you please add me to the science feed. Thanks in advance!

orcid.org/0000-0001-91...
www.kispi.uzh.ch/forschungsze...

Hi @andreasmoor.bsky.social can I also be added to the spatial biology starter pack. thanks

Hi @giorgioseano.bsky.social I would love to be added to this starter pack as it is my current research field. Thank you

@lkpino.bsky.social would you agree on the name tag or think switching would make more sense?

While the Imaging mass spectrometry (IMS)-based is defined as Top-down proteomics analysing intact proteins, while bottom-up proteomics digests proteins into peptides before analysis.
But I do agree with you, also when we say pixel we all intuitively think imaging.

This figure is from a paper [source in the alt text] where they combine LC-MS/MS with with micro-manipulation techniques, such as laser capture microdissection (LCM) and that can be done on ROI or pixel by pixel level

1️⃣ Resolution – How detailed do you need? Higher resolution = more precision, but can be complex.
2️⃣ Targeted proteins – Focused project or exploratory?
3️⃣ Resources – What tools/funding are available?

Your choices shape your research! 🔍 What other variables should we consider?
#Computational #Biology