The bumbling biochemist (Bri Bibel)
@bumblingbiochemist.bsky.social
Assistant Prof of Biochemistry & Chemistry @ LMU | Metabolic enzymes of bioremediating bacteria. Making biochemistry fun & accessible #biochemsky #chemsky
http://thebumblingbiochemist.com/ & https://www.youtube.com/@thebumblingbiochemist
http://thebumblingbiochemist.com/ & https://www.youtube.com/@thebumblingbiochemist
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biochemistry (hopefully) made accessible!
You can also find lots of videos on my YouTube channel
here - as in life - feel free to explore!
My site is constantly a work in progress, so...
thebumblingbiochemist.com
Hi all. I'm the bumbling biochemist, on a mission to make biochemistry and chemistry fun and accessible for all. I have a blog & YouTube with a lot of content that can hopefully help people, especially biochemists-in-training. Hope it helps! thebumblingbiochemist.com
Getting your sample on the AKTA: Sample Loops and Sample Pumps
Bottom line: Sample loops are good for small volumes, sample pumps for large volumes, but the pumps are expensive. Superloops are an option for larger volumes without a pump, but they're kinda a pain
youtu.be/OkgW7RrVuXA
Bottom line: Sample loops are good for small volumes, sample pumps for large volumes, but the pumps are expensive. Superloops are an option for larger volumes without a pump, but they're kinda a pain
youtu.be/OkgW7RrVuXA
Getting your sample on the AKTA: Sample Loops and Sample Pumps
YouTube video by the bumbling biochemist
youtu.be
November 15, 2025 at 3:24 AM
Getting your sample on the AKTA: Sample Loops and Sample Pumps
Bottom line: Sample loops are good for small volumes, sample pumps for large volumes, but the pumps are expensive. Superloops are an option for larger volumes without a pump, but they're kinda a pain
youtu.be/OkgW7RrVuXA
Bottom line: Sample loops are good for small volumes, sample pumps for large volumes, but the pumps are expensive. Superloops are an option for larger volumes without a pump, but they're kinda a pain
youtu.be/OkgW7RrVuXA
Let's go on a rapid roadtrip through the heart of human metabolism!
Feel free to download and use this map – please cite if you do, thanks!
youtu.be/-x8S4SSbn58
Bibel, B., & The bumbling biochemist. (2024). Core Metabolic Roadmap. Zenodo. zenodo.org/doi/10.5281/...
Feel free to download and use this map – please cite if you do, thanks!
youtu.be/-x8S4SSbn58
Bibel, B., & The bumbling biochemist. (2024). Core Metabolic Roadmap. Zenodo. zenodo.org/doi/10.5281/...
Metabolic roadtrip: A whirlwind overview of the fundamentals of central metabolism
YouTube video by the bumbling biochemist
youtu.be
November 13, 2025 at 2:37 AM
Let's go on a rapid roadtrip through the heart of human metabolism!
Feel free to download and use this map – please cite if you do, thanks!
youtu.be/-x8S4SSbn58
Bibel, B., & The bumbling biochemist. (2024). Core Metabolic Roadmap. Zenodo. zenodo.org/doi/10.5281/...
Feel free to download and use this map – please cite if you do, thanks!
youtu.be/-x8S4SSbn58
Bibel, B., & The bumbling biochemist. (2024). Core Metabolic Roadmap. Zenodo. zenodo.org/doi/10.5281/...
PCR (Polymerase Chain Reaction) overview, design, and troubleshooting tips youtu.be/DIiRT8OuzJ8
PCR (Polymerase Chain Reaction) overview, design, and troubleshooting tips
YouTube video by the bumbling biochemist
youtu.be
November 11, 2025 at 11:19 PM
PCR (Polymerase Chain Reaction) overview, design, and troubleshooting tips youtu.be/DIiRT8OuzJ8
In the lab, X-marks the relative concentration! youtu.be/gigGrPm5CyU
Working with x-factors of relative concentrations of stock & working solutions (e.g. 6X loading dye)
YouTube video by the bumbling biochemist
youtu.be
November 11, 2025 at 5:15 AM
In the lab, X-marks the relative concentration! youtu.be/gigGrPm5CyU
Take a hint! Key word roots etc. that will help you figure out lots about biochemical reactions from names of molecules and enzymes alone! youtu.be/OtKkzX15ikw
What's in a name? A lot! How to get the most info you can out of enzyme, molecule, & pathway names
YouTube video by the bumbling biochemist
youtu.be
November 9, 2025 at 11:33 PM
Take a hint! Key word roots etc. that will help you figure out lots about biochemical reactions from names of molecules and enzymes alone! youtu.be/OtKkzX15ikw
Uniprot vs. the PDB: Understanding their entries and differences
Bottom line:
* Each entry in Uniprot corresponds to a protein (chain)
* Each entry in the PDB (Protein DataBank) corresponds to a structural model
Bottom line:
* Each entry in Uniprot corresponds to a protein (chain)
* Each entry in the PDB (Protein DataBank) corresponds to a structural model
Uniprot vs. the PDB: Understanding their entries and differences
YouTube video by the bumbling biochemist
youtu.be
November 8, 2025 at 11:50 PM
Uniprot vs. the PDB: Understanding their entries and differences
Bottom line:
* Each entry in Uniprot corresponds to a protein (chain)
* Each entry in the PDB (Protein DataBank) corresponds to a structural model
Bottom line:
* Each entry in Uniprot corresponds to a protein (chain)
* Each entry in the PDB (Protein DataBank) corresponds to a structural model
You finally got your PCR to work? Great! Now, clean up your mess! youtu.be/3FyGRAlbv_8 blog version (text old, video new): bit.ly/spincolumns
PCR product purification/clean-up (eg. QIAquick PCR Purification) & other nucleic acid spin columns
YouTube video by the bumbling biochemist
youtu.be
November 8, 2025 at 4:41 AM
You finally got your PCR to work? Great! Now, clean up your mess! youtu.be/3FyGRAlbv_8 blog version (text old, video new): bit.ly/spincolumns
If you've got glucose, you've got options! Glycolysis tends to get all the spotlight, but that's just one way to go! (At least until you reach a one-way arrow!)
More on metabolism: bit.ly/metabolismgl... & bit.ly/bbmetabolism
More on metabolism: bit.ly/metabolismgl... & bit.ly/bbmetabolism
November 7, 2025 at 2:06 AM
If you've got glucose, you've got options! Glycolysis tends to get all the spotlight, but that's just one way to go! (At least until you reach a one-way arrow!)
More on metabolism: bit.ly/metabolismgl... & bit.ly/bbmetabolism
More on metabolism: bit.ly/metabolismgl... & bit.ly/bbmetabolism
Quick lab tip: Use magnetic clips to clip your protocol above you as you. You can use metal tape to create surfaces if needed and allowed. Unlike just taping up your protocol, with clips you can easily flip through things to see what's coming up, change ordering, etc.
youtube.com/shorts/Qw93p...
youtube.com/shorts/Qw93p...
Quick lab tip: Use magnetic clips to clip your protocol above you as you work
YouTube video by the bumbling biochemist
youtube.com
November 6, 2025 at 2:24 AM
Quick lab tip: Use magnetic clips to clip your protocol above you as you. You can use metal tape to create surfaces if needed and allowed. Unlike just taping up your protocol, with clips you can easily flip through things to see what's coming up, change ordering, etc.
youtube.com/shorts/Qw93p...
youtube.com/shorts/Qw93p...
Electron Transport Chain and Oxidative Phosphorylation practice problems (uncouplers, low O2, etc.) & review youtu.be/cne0QtdKPOo
Made for my class, so your mileage may vary, but hope this can help someone.
Made for my class, so your mileage may vary, but hope this can help someone.
Electron Transport Chain and Oxidative Phosphorylation practice (uncouplers, low O2, etc.) & review
YouTube video by the bumbling biochemist
youtu.be
November 5, 2025 at 12:32 AM
Electron Transport Chain and Oxidative Phosphorylation practice problems (uncouplers, low O2, etc.) & review youtu.be/cne0QtdKPOo
Made for my class, so your mileage may vary, but hope this can help someone.
Made for my class, so your mileage may vary, but hope this can help someone.
Our reusable bottle-top vacuum filter finally came! And I honestly don’t know who was more excited: me or the students I have the privilege of working with! Huge props to Korina, Liam, and Ryan!
youtu.be/buV1_E4APBs
youtu.be/buV1_E4APBs
Tips for setting up and using reusable bottle-top vacuum filters
YouTube video by the bumbling biochemist
youtu.be
November 4, 2025 at 4:28 AM
Our reusable bottle-top vacuum filter finally came! And I honestly don’t know who was more excited: me or the students I have the privilege of working with! Huge props to Korina, Liam, and Ryan!
youtu.be/buV1_E4APBs
youtu.be/buV1_E4APBs
Metabolic thermodynamics, metabolic logic, and metabolic pathway diagram notation: review
Metabolic thermodynamics, metabolic logic, & pathway diagram notation: review & practice Qs
YouTube video by the bumbling biochemist
youtu.be
November 2, 2025 at 11:41 PM
Metabolic thermodynamics, metabolic logic, and metabolic pathway diagram notation: review
Biochemistry outlines/study guides adapted from ones I give my undergraduate Biochemistry students. Your mileage might vary, but thought these might help people so thought I'd share.
thebumblingbiochemist.com/biochemistry...
thebumblingbiochemist.com/biochemistry...
Biochemistry outlines/study guides
Adapted from ones I give my undergraduate Biochemistry students. Your mileage might vary, but thought these might help people so thought I'd share.
Functional groups, InterMolecular forces (IMFs)...
thebumblingbiochemist.com
November 1, 2025 at 10:39 PM
Biochemistry outlines/study guides adapted from ones I give my undergraduate Biochemistry students. Your mileage might vary, but thought these might help people so thought I'd share.
thebumblingbiochemist.com/biochemistry...
thebumblingbiochemist.com/biochemistry...
Outline of biosignaling, membrane transport, metabolic thermodynamics, biochemical redox, the electron transport chain (ETC) and oxidative phosphorylation (oxphos)
Adapted from a version I give my Biochemistry undergraduate students. drive.google.com/file/d/1mEQQ...
Adapted from a version I give my Biochemistry undergraduate students. drive.google.com/file/d/1mEQQ...
Biochem_Study_Guide_3_Public.pdf
drive.google.com
November 1, 2025 at 8:41 PM
Outline of biosignaling, membrane transport, metabolic thermodynamics, biochemical redox, the electron transport chain (ETC) and oxidative phosphorylation (oxphos)
Adapted from a version I give my Biochemistry undergraduate students. drive.google.com/file/d/1mEQQ...
Adapted from a version I give my Biochemistry undergraduate students. drive.google.com/file/d/1mEQQ...
When it comes to multichannel pipettes, evenness is the name of the game! Apply even, vertical pressure to make sure your liquid levels are the same!
So fun teaching Alice, Korina, and Liam to use them and putting them to exciting use for an experiment!
youtu.be/ZEjBtAFmb_8
So fun teaching Alice, Korina, and Liam to use them and putting them to exciting use for an experiment!
youtu.be/ZEjBtAFmb_8
Tips for using multichannel pipets
YouTube video by the bumbling biochemist
youtu.be
November 1, 2025 at 1:48 AM
When it comes to multichannel pipettes, evenness is the name of the game! Apply even, vertical pressure to make sure your liquid levels are the same!
So fun teaching Alice, Korina, and Liam to use them and putting them to exciting use for an experiment!
youtu.be/ZEjBtAFmb_8
So fun teaching Alice, Korina, and Liam to use them and putting them to exciting use for an experiment!
youtu.be/ZEjBtAFmb_8
Save that sleeve! When you open up a sleeve of Petri dishes, do so carefully from the top and save the bag to use as a storage sleeve.
youtube.com/shorts/qjx3p...
youtube.com/shorts/qjx3p...
Save that sleeve! Lab tips for bagging agar plates
YouTube video by the bumbling biochemist
youtube.com
October 31, 2025 at 2:49 AM
Save that sleeve! When you open up a sleeve of Petri dishes, do so carefully from the top and save the bag to use as a storage sleeve.
youtube.com/shorts/qjx3p...
youtube.com/shorts/qjx3p...
Redox time in biochem class . . . M&M's came out in full force yesterday
October 30, 2025 at 12:43 PM
Redox time in biochem class . . . M&M's came out in full force yesterday
Repeater pipets are surprisingly under-used and appreciated in my experience. But Korina and Ryan loved using them and definitely appreciated them! I love being able to introduce undergrads to wonderful lab life! And I definitely appreciate them (much more than the pipets :) ) youtu.be/KgwlmDRlS4s
Using a repeater pipet
YouTube video by the bumbling biochemist
youtu.be
October 30, 2025 at 2:37 AM
Repeater pipets are surprisingly under-used and appreciated in my experience. But Korina and Ryan loved using them and definitely appreciated them! I love being able to introduce undergrads to wonderful lab life! And I definitely appreciate them (much more than the pipets :) ) youtu.be/KgwlmDRlS4s
My favorite pipette: p10. And it came :)
YouTube video by the bumbling biochemist
youtube.com
October 29, 2025 at 12:28 AM
When you’re too short for your whiteboard . . . (caught in the act on our classroom recording system…)
October 28, 2025 at 2:06 AM
When you’re too short for your whiteboard . . . (caught in the act on our classroom recording system…)
Don’t confuse e.g. with i.e. or et al. or etc. (and use them all properly!) youtu.be/L6yz23HWhbc
Don’t confuse e.g. with i.e. or et al. or etc. (and use them all properly!)
YouTube video by the bumbling biochemist
youtu.be
October 26, 2025 at 10:46 PM
Don’t confuse e.g. with i.e. or et al. or etc. (and use them all properly!) youtu.be/L6yz23HWhbc
Periodic PSA: "western" should be lowercase in western blot – unlike the "Southern" blot, which was named for a person, the western blot was named for a laugh
October 26, 2025 at 2:38 PM
Periodic PSA: "western" should be lowercase in western blot – unlike the "Southern" blot, which was named for a person, the western blot was named for a laugh
A walkthrough of some of my favorite science nonfiction (mostly biochemistry-related)
youtu.be/1uLh-OzZSaQ
youtu.be/1uLh-OzZSaQ
Some of my favorite science nonfiction (mostly biochemistry-related)
YouTube video by the bumbling biochemist
youtu.be
October 25, 2025 at 10:52 PM
A walkthrough of some of my favorite science nonfiction (mostly biochemistry-related)
youtu.be/1uLh-OzZSaQ
youtu.be/1uLh-OzZSaQ
Lab life's ambidextrous: one handed tube handling. . . I love getting to help build undergrad's lab technique skills!
youtube.com/shorts/R-SCY...
youtube.com/shorts/R-SCY...
Lab life's ambidextrous: one handed tube handling. . .
YouTube video by the bumbling biochemist
youtube.com
October 25, 2025 at 1:52 AM
Lab life's ambidextrous: one handed tube handling. . . I love getting to help build undergrad's lab technique skills!
youtube.com/shorts/R-SCY...
youtube.com/shorts/R-SCY...
