Bastian B.
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Bastian B.
@bastibu.bsky.social
230 followers 120 following 1 posts
Chemical Biologist.
RNA Imaging. Biosensors. Bioassays.
Small fluorophores that label biomolecules.
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Reposted by Bastian B.
juliankompa.bsky.social
Productive weeks at the Chemical Biology department at @mpi-mr @kjohnsson 🎉
Check out the fantastic work by @steffi-k on SNAP-tag2 and @deensun3 on KinProLa in @natchembio.nature.com ⏬

We also released a great collab with @zhixingchen2 on the photophysics of self-labeling tags in @pnas.org. (1/4)
July 29, 2025 at 7:24 AM
Reposted by Bastian B.
mpi-mr.bsky.social
📣Paper alert! 👏 If you're interested in investigating kinase activities in living cells, our scientists @kjohnsson.bsky.social have built a molecular recorder to monitor them spatially and temporally. Check out our website or Nature Chemical Biology (link in comments)!
www.mr.mpg.de/14652710/mol...
Investigating kinase activity in living cells
www.mr.mpg.de
July 10, 2025 at 10:32 AM
Reposted by Bastian B.
kjohnsson.bsky.social
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
www.nature.com
July 3, 2025 at 7:45 PM
Reposted by Bastian B.
kjohnsson.bsky.social
Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging:

www.biorxiv.org/content/10.1...

Congratulations to Julian Kompa and the entire team.
Fast, Bright and Reversible Rhodamine Tags for Live-Cell Imaging
We present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...
www.biorxiv.org
July 9, 2025 at 7:58 PM
Reposted by Bastian B.
mpi-mr.bsky.social
📣Paper alert! Interested in faster and brighter protein labeling than before? 👀 @steffi-k.bsky.social & other colleagues have developed an improved tool for labeling proteins with synthetic fluorophores for bioimaging. Also works in STED microscopy 🤩🔬 Congratulations!🥳
www.nature.com/articles/s41...
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
www.nature.com
July 3, 2025 at 12:09 PM
Reposted by Bastian B.
kjohnsson.bsky.social
Please check out our new high-affinity split-HaloTag for live-cell protein labeling www.biorxiv.org/content/10.1...
Congratulations to Yin-Hsi Lin!
www.biorxiv.org
June 16, 2025 at 2:24 PM
Reposted by Bastian B.
kjohnsson.bsky.social
Improving split-HaloTag through computational protein engineering:

onlinelibrary.wiley.com/doi/10.1002/...
Improving split‐HaloTag through computational protein engineering
Split-HaloTag can be used to transform transient molecular interactions into permanent marks through chemical labeling, thereby enabling the recording of transient physiological events in individual ....
onlinelibrary.wiley.com
April 18, 2025 at 8:17 PM
Reposted by Bastian B.
veselin-nasufovic.bsky.social
1/5 🚀 Excited to share our latest preprint from: 𝗦𝗶𝗥-𝗫𝗔𝗰𝘁𝗶𝗻: 𝗔 𝗳𝗹𝘂𝗼𝗿𝗲𝘀𝗰𝗲𝗻𝘁 𝗽𝗿𝗼𝗯𝗲 𝗳𝗼𝗿 𝗶𝗺𝗮𝗴𝗶𝗻𝗴 𝗮𝗰𝘁𝗶𝗻 𝗱𝘆𝗻𝗮𝗺𝗶𝗰𝘀 𝗶𝗻 𝗹𝗶𝘃𝗲 𝗰𝗲𝗹𝗹𝘀 on #bioRxiv!
www.biorxiv.org/content/10.1...

#JohnssonLab and #ArndtLab with valued collaborators
February 6, 2025 at 8:00 PM
Reposted by Bastian B.
marvintanenbaum.bsky.social
Our new paper is out: "Mapping the complete influenza A virus infection cycle through single vRNP imaging". Combining newly-developed single-molecule imaging approaches with in situ viral transcriptomics, we identify numerous non-canonical infection pathways.

www.biorxiv.org/content/10.1...
Mapping the complete influenza A virus infection cycle through single vRNP imaging
Cell-to-cell heterogeneity is a common feature of viral infection that can generate enormous complexity, complicating understanding of infection progression and interpretation of differences between v...
www.biorxiv.org
January 21, 2025 at 9:56 AM
Reposted by Bastian B.
juliankompa.bsky.social
Hi Bluesky! 👋
Here is our latest collaborative work on long-term (up to 20 min) single-molecule tracking in living cells using exchangeable HaloTag Ligands 🔬
#ChemBio #microscopy

onlinelibrary.wiley.com/doi/10.1002/...
Long‐Term Single‐Molecule Tracking in Living Cells using Weak‐Affinity Protein Labeling
A self-labeling protein tag in combination with exchangeable fluorophore ligands bypasses photobleaching and enables long-term single-molecule imaging in living cells. This extends observation times ...
onlinelibrary.wiley.com
November 27, 2024 at 8:24 AM