Nick Ariotti
@ariottilab.bsky.social
Cell Biologist; Membrane Biologist; Electron Microscopist; Endocytosis-ist; Cryo-CLEM-ist; Cryo-ET-er; Structural Biologist-ish; Virologist-ish; He/Him
Reposted by Nick Ariotti
🧵In collaboration with Caroline Puente-Lelievre, Ed Moody, Rosie Cater, and Tom Williams we just submitted a new paper describing the prokaryotic origins of the COMMD protein family. @cpuentelelievre.bsky.social, @rosemaryjcater.bsky.social. @kmichie.bsky.social. 1/n
The prokaryotic origins of the COMMD protein family involved in eukaryotic membrane trafficking
The ten eukaryotic COMMD proteins are core components of the Commander complex, with central roles in endosomal membrane trafficking and signalling. Each protein has an α-helical N-terminal (HN) domai...
www.biorxiv.org
September 3, 2025 at 12:24 AM
🧵In collaboration with Caroline Puente-Lelievre, Ed Moody, Rosie Cater, and Tom Williams we just submitted a new paper describing the prokaryotic origins of the COMMD protein family. @cpuentelelievre.bsky.social, @rosemaryjcater.bsky.social. @kmichie.bsky.social. 1/n
Reposted by Nick Ariotti
rdcu.be/dY5rl
The latest research article from Ti Lab. We employed cryo-EM structural analysis, single-particle fluorescence microscopy, and recombinant tubulin to reveal how tubulin acetyltransferases modify the K40 residue inside the microtubule lumen.
The latest research article from Ti Lab. We employed cryo-EM structural analysis, single-particle fluorescence microscopy, and recombinant tubulin to reveal how tubulin acetyltransferases modify the K40 residue inside the microtubule lumen.
Tubulin acetyltransferases access and modify the microtubule luminal K40 residue through anchors in taxane-binding pockets
Nature Structural & Molecular Biology - Here, the authors reveal the mechanism by which anchors of a tubulin acetyltransferase in the β-tubulin taxane-binding pockets have critical roles...
rdcu.be
December 27, 2024 at 3:06 AM
rdcu.be/dY5rl
The latest research article from Ti Lab. We employed cryo-EM structural analysis, single-particle fluorescence microscopy, and recombinant tubulin to reveal how tubulin acetyltransferases modify the K40 residue inside the microtubule lumen.
The latest research article from Ti Lab. We employed cryo-EM structural analysis, single-particle fluorescence microscopy, and recombinant tubulin to reveal how tubulin acetyltransferases modify the K40 residue inside the microtubule lumen.
Reposted by Nick Ariotti
Have you ever wondered whether pyroptotic corpses have functions beyond the grave? We made the surprising discovery that pyroptotic corpses are flagged by #filopodia to alert #DendriticCells they need autopsy. A short Bluetorial (1/13)👇with movies!
Link: rdcu.be/d2mjm
Link: rdcu.be/d2mjm
Pyroptotic cell corpses are crowned with F-actin-rich filopodia that engage CLEC9A signaling in incoming dendritic cells
Nature Immunology - Pyroptotic cell death results in inflammation. Here the authors find that F-actin-rich structures formed during macrophage pyroptosis persist after cell death to activate...
rdcu.be
December 9, 2024 at 1:39 AM
Have you ever wondered whether pyroptotic corpses have functions beyond the grave? We made the surprising discovery that pyroptotic corpses are flagged by #filopodia to alert #DendriticCells they need autopsy. A short Bluetorial (1/13)👇with movies!
Link: rdcu.be/d2mjm
Link: rdcu.be/d2mjm
Reposted by Nick Ariotti
Lo and behold, the structure of a "lipid raft":
"our structures suggest that specific sphingomyelin–cholesterol interactions do not play a critical role in AQP0 array formation in the lens membrane."
Chiu PL et al. Elife. 2024. doi.org/10.7554/eLif...
🧪
"our structures suggest that specific sphingomyelin–cholesterol interactions do not play a critical role in AQP0 array formation in the lens membrane."
Chiu PL et al. Elife. 2024. doi.org/10.7554/eLif...
🧪
Structure and dynamics of cholesterol-mediated aquaporin-0 arrays and implications for lipid rafts
Electron crystallography and molecular dynamics simulations show that a cholesterol sandwiched in between two neighboring aquaporin-0 tetramers stabilizes the association between the tetramers and thu...
doi.org
December 5, 2024 at 8:53 PM
Lo and behold, the structure of a "lipid raft":
"our structures suggest that specific sphingomyelin–cholesterol interactions do not play a critical role in AQP0 array formation in the lens membrane."
Chiu PL et al. Elife. 2024. doi.org/10.7554/eLif...
🧪
"our structures suggest that specific sphingomyelin–cholesterol interactions do not play a critical role in AQP0 array formation in the lens membrane."
Chiu PL et al. Elife. 2024. doi.org/10.7554/eLif...
🧪