LOV-BirA, light regulated biotin ligase, engineered by Song-Yi Lee

www.sciencedirect.com/science/arti...

LOV-BirA, light regulated biotin ligase, engineered by Song-Yi Lee

www.sciencedirect.com/science/arti...

Today we report the directed evolution of HUH into rHUH, a sequence-specific covalent protein tag for RNA labeling. rHUH was hard to engineer: the initial HUH template had no RNA activity, so we started with RNA-DNA hybrids. 30 rounds of selection on the yeast surface were required, and 7 cycles of

Yes we did a side by side comparison to PA-BoNT in Figure S7A and found that PA-BoNT showed much lower +light activity and higher dark-state background than LATeNT

Very proud of amazing protein engineer Heegwang Roh, our fantastic neuroscience collaborator Ji Won Um, co-first author Dongwook Kim from the Um lab, and our other wonderful co-authors.

Tetanus on demand! (not for bioweapons, but for neuroscience research)

Tetanus toxin induces paralysis by using a protease to cleave VAMP2, a protein required for synaptic vesicle fusion and neurotransmitter release.

To harness this toxicity and use it as a beneficial tool for neuroscience,

a real-time fluorescence response to antigen binding. And PAGER-G couples antigen recognition to activation of endogenous G protein pathways. Because there are four major classes of G proteins, we created four PAGER-G subtypes, for controlling endogenous Gi, Gs, Gq, or G12.

with the antagonist, leading to relief of receptor inhibition. Drug or agonist can then turn on PAGER.

www.nature.com/articles/s41...