LOV-BirA, light regulated biotin ligase, engineered by Song-Yi Lee
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
August 27, 2025 at 3:30 PM
LOV-BirA, light regulated biotin ligase, engineered by Song-Yi Lee
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
LOV-BirA, light regulated biotin ligase, engineered by Song-Yi Lee
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
August 27, 2025 at 3:27 PM
LOV-BirA, light regulated biotin ligase, engineered by Song-Yi Lee
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
subjects with at least 1 year of prior wetlab research experience. Must be a US citizen or have a valid work visa.
To apply: careersearch.stanford.edu/jobs/life-sc...
To apply: careersearch.stanford.edu/jobs/life-sc...
Life Science Research Professional 1 in School of Medicine, Stanford, California, United States
We seek motivated recent college graduates with substantial research experience who are looking for a launching pad into the next phase of their...
careersearch.stanford.edu
March 26, 2025 at 5:40 PM
subjects with at least 1 year of prior wetlab research experience. Must be a US citizen or have a valid work visa.
To apply: careersearch.stanford.edu/jobs/life-sc...
To apply: careersearch.stanford.edu/jobs/life-sc...
keeping the lab running (being a lab manager), and 50% is for original research related to protein engineering, technology development, etc – there are many project possibilities!
Seeking passionate, motivated, creative, and detail-oriented majors in chemical biology, molecular biology or related..
Seeking passionate, motivated, creative, and detail-oriented majors in chemical biology, molecular biology or related..
March 26, 2025 at 5:39 PM
keeping the lab running (being a lab manager), and 50% is for original research related to protein engineering, technology development, etc – there are many project possibilities!
Seeking passionate, motivated, creative, and detail-oriented majors in chemical biology, molecular biology or related..
Seeking passionate, motivated, creative, and detail-oriented majors in chemical biology, molecular biology or related..
applications.
Congratulations to postdoc Rongbing Huang, whose creativity, persistence, and resourcefulness made rHUH possible! We are grateful to NSF and Biohub for funding this work.
www.pnas.org/doi/10.1073/pnas.2422085122
Congratulations to postdoc Rongbing Huang, whose creativity, persistence, and resourcefulness made rHUH possible! We are grateful to NSF and Biohub for funding this work.
www.pnas.org/doi/10.1073/pnas.2422085122
Directed evolution of a sequence-specific covalent protein tag for RNA labeling | PNAS
Efficient methods for conjugating proteins to RNA are needed for RNA delivery, imaging,
editing, interactome mapping, and barcoding applications. N...
www.pnas.org
February 26, 2025 at 11:23 PM
applications.
Congratulations to postdoc Rongbing Huang, whose creativity, persistence, and resourcefulness made rHUH possible! We are grateful to NSF and Biohub for funding this work.
www.pnas.org/doi/10.1073/pnas.2422085122
Congratulations to postdoc Rongbing Huang, whose creativity, persistence, and resourcefulness made rHUH possible! We are grateful to NSF and Biohub for funding this work.
www.pnas.org/doi/10.1073/pnas.2422085122
and imperfect sequence specificity resulting in significant background labeling of endogenous transcripts.
There is still work to do for this to be a dream tool for RNA imaging/editing/mapping in living cells, but rHUH provides a good starting point and it is definitely good enough for in vitro
There is still work to do for this to be a dream tool for RNA imaging/editing/mapping in living cells, but rHUH provides a good starting point and it is definitely good enough for in vitro
February 26, 2025 at 11:22 PM
and imperfect sequence specificity resulting in significant background labeling of endogenous transcripts.
There is still work to do for this to be a dream tool for RNA imaging/editing/mapping in living cells, but rHUH provides a good starting point and it is definitely good enough for in vitro
There is still work to do for this to be a dream tool for RNA imaging/editing/mapping in living cells, but rHUH provides a good starting point and it is definitely good enough for in vitro
library diversification.
The final rHUH tag is 13.4 kD, has 12 mutations relative to HUH, and covalently labels as little as 1 nM target RNA within minutes. The labeling works well in mammalian cell lysate, but not yet in the interior of living cells – possibly due to the high Mg2+ requirement
The final rHUH tag is 13.4 kD, has 12 mutations relative to HUH, and covalently labels as little as 1 nM target RNA within minutes. The labeling works well in mammalian cell lysate, but not yet in the interior of living cells – possibly due to the high Mg2+ requirement
February 26, 2025 at 11:22 PM
library diversification.
The final rHUH tag is 13.4 kD, has 12 mutations relative to HUH, and covalently labels as little as 1 nM target RNA within minutes. The labeling works well in mammalian cell lysate, but not yet in the interior of living cells – possibly due to the high Mg2+ requirement
The final rHUH tag is 13.4 kD, has 12 mutations relative to HUH, and covalently labels as little as 1 nM target RNA within minutes. The labeling works well in mammalian cell lysate, but not yet in the interior of living cells – possibly due to the high Mg2+ requirement
There is no detectable toxicity in the dark state because the protease activity is completely shut off. After activation, LATeNT only cleaves VAMP2, inhibiting synaptic transmission, but it does not affect other pathways. It does not kill cells.
January 31, 2025 at 5:03 AM
There is no detectable toxicity in the dark state because the protease activity is completely shut off. After activation, LATeNT only cleaves VAMP2, inhibiting synaptic transmission, but it does not affect other pathways. It does not kill cells.
Yes we did a side by side comparison to PA-BoNT in Figure S7A and found that PA-BoNT showed much lower +light activity and higher dark-state background than LATeNT
January 30, 2025 at 4:44 PM
Yes we did a side by side comparison to PA-BoNT in Figure S7A and found that PA-BoNT showed much lower +light activity and higher dark-state background than LATeNT
Very proud of amazing protein engineer Heegwang Roh, our fantastic neuroscience collaborator Ji Won Um, co-first author Dongwook Kim from the Um lab, and our other wonderful co-authors.
January 30, 2025 at 2:37 PM
Very proud of amazing protein engineer Heegwang Roh, our fantastic neuroscience collaborator Ji Won Um, co-first author Dongwook Kim from the Um lab, and our other wonderful co-authors.
24 hours after shutting off LATeNT by taking away the light, anxiety levels returned to normal.
We are excited for neuroscientists to try this new tool! The inhibitory effect is stronger and more sustained than with other tools like halorhodopsin and DREADDs, but the timing and location of the...
We are excited for neuroscientists to try this new tool! The inhibitory effect is stronger and more sustained than with other tools like halorhodopsin and DREADDs, but the timing and location of the...
January 30, 2025 at 2:31 PM
24 hours after shutting off LATeNT by taking away the light, anxiety levels returned to normal.
We are excited for neuroscientists to try this new tool! The inhibitory effect is stronger and more sustained than with other tools like halorhodopsin and DREADDs, but the timing and location of the...
We are excited for neuroscientists to try this new tool! The inhibitory effect is stronger and more sustained than with other tools like halorhodopsin and DREADDs, but the timing and location of the...
Synaptic inhibition is potently inhibited only in those cells expressing LATeNT while neighboring cells are unaffected.
We used LATeNT in the mouse brain to discover a new cell population (SST+ interneurons in the hippocampus) that modulates anxiety.
We used LATeNT in the mouse brain to discover a new cell population (SST+ interneurons in the hippocampus) that modulates anxiety.
January 30, 2025 at 2:31 PM
Synaptic inhibition is potently inhibited only in those cells expressing LATeNT while neighboring cells are unaffected.
We used LATeNT in the mouse brain to discover a new cell population (SST+ interneurons in the hippocampus) that modulates anxiety.
We used LATeNT in the mouse brain to discover a new cell population (SST+ interneurons in the hippocampus) that modulates anxiety.
we fused a light-sensitive LOV domain from oat plants into tetanus’ protease domain and made its activity controllable by light.
LATeNT (for light-activated tetanus neurotoxin) can be expressed in specific cell populations, and then turned on with a brief pulse of blue light.
LATeNT (for light-activated tetanus neurotoxin) can be expressed in specific cell populations, and then turned on with a brief pulse of blue light.
January 30, 2025 at 2:30 PM
we fused a light-sensitive LOV domain from oat plants into tetanus’ protease domain and made its activity controllable by light.
LATeNT (for light-activated tetanus neurotoxin) can be expressed in specific cell populations, and then turned on with a brief pulse of blue light.
LATeNT (for light-activated tetanus neurotoxin) can be expressed in specific cell populations, and then turned on with a brief pulse of blue light.
...research labs that are part of the CZ Biohub network (including mine!).
See czbiohub.org/program-ssfc... to learn more and email me with any questions
Applications accepted on a rolling basis until March 27, 2025
See czbiohub.org/program-ssfc... to learn more and email me with any questions
Applications accepted on a rolling basis until March 27, 2025
Global Science Scholars Postdoctoral Fellowship Program SS-F
Apply for the new international postdoctoral fellowship program from the CZ Biohub Network and Stellar Science Foundation.
czbiohub.org
January 9, 2025 at 6:22 PM
...research labs that are part of the CZ Biohub network (including mine!).
See czbiohub.org/program-ssfc... to learn more and email me with any questions
Applications accepted on a rolling basis until March 27, 2025
See czbiohub.org/program-ssfc... to learn more and email me with any questions
Applications accepted on a rolling basis until March 27, 2025
activation migration, etc). This is discussed in the paper
December 4, 2024 at 10:19 PM
activation migration, etc). This is discussed in the paper