Escribime por DM. Si querés ver lo que hacemos visítanos en:
asoler1.wixsite.com/iib-geb

I thank my HUGE collaborators: @amazeld.bsky.social @celineloot.bsky.social @val-meve.bsky.social Briardo Llorente at @pasteur.fr and Macquarie University. The funds and institutions supporting the project @unsamoficial.bsky.social CONICET Agencia I+D+i

Hence, evolution maintains RNAP near oriC highlighting the key role gene order with implications in ecological strategies, adaptation, and synthetic genome design. We provide an experimental framework for investigating how genome architecture shapes cellular function.

The rpoB and rpoC genes are part of a large locus, and being the last ORF of the operon. To show that the phenotypes observed were consequence exclusively of RNA polymerase genes genomic location, we disected the locus.

We then added a second copy of the locus to show that the pheno typic effects are mostly due to alteration in replication associated rpoBC dosage reduction.

We built an rpoC-mCherry translational fusion that allowed us to observe that the distribution is not disturbed upon relocation. However, we notice less RpoC-mCherry protein when the locus is far from oriC.