Budget cuts do not curb our enthusiasm! Ian lab meeting today on Bradyrhizobium cell physiology! #LabMeetinDay

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Estas en 3-4° año de biotecnología o carreras afines? Estamos buscando un/a estudiante de @unsamoficial.bsky.social para incorporar al laboratorio y presentar a una beca de entrenamiento de la CIC PBA! Te interesa? Vení a trabajar con nosotros!! #UNSAM #Biotecnologia !!

Submitted! Fingers crossed!!

The rpoB and rpoC genes are part of a large locus, and being the last ORF of the operon. To show that the phenotypes observed were consequence exclusively of RNA polymerase genes genomic location, we disected the locus.

We then added a second copy of the locus to show that the pheno typic effects are mostly due to alteration in replication associated rpoBC dosage reduction.

We built an rpoC-mCherry translational fusion that allowed us to observe that the distribution is not disturbed upon relocation. However, we notice less RpoC-mCherry protein when the locus is far from oriC.

When rpoBC is far from oriC, growth rate and dosage decreases.

Bacteria harbour a single RNAP encoded by rpoBC genes. In fast-growers it is conserver close to oriC (bsky.app/profile/did:...). We relocated rpoBC (in red) to different locations within Vibrio cholerae genome:

Gran Curso de Biología Vegetal en Chile! Coordina el gran @estevezjosem.bsky.social

A new Argentinean Society for General Microbiology (SAMiGe) Board starts. My new role is General secretary. We will do our best effort to promote fundamental microbiology in 🇦🇷 and the region in this challenging context!