Alba Hykollari
@albahykolla.bsky.social
Reposted by Alba Hykollari
I guess we need more unusual glycan standards, e.g., with GlcA or PC, to increase coverage of, e.g., insect structures! The 83% for MMF seems high for Sf9-produced Spike, unless the BEI stuff is very different from Novavax.
June 11, 2025 at 9:10 PM
I guess we need more unusual glycan standards, e.g., with GlcA or PC, to increase coverage of, e.g., insect structures! The 83% for MMF seems high for Sf9-produced Spike, unless the BEI stuff is very different from Novavax.
Reposted by Alba Hykollari
We checked with untargeted SUGA to be sure (negative mode only), but only saw the more simple structures compared to what you’ve reported.
There’s a chance there was further processing involved of the spike protein by the manufacturer (like ion exchange) which may have depleted charged modifications
There’s a chance there was further processing involved of the spike protein by the manufacturer (like ion exchange) which may have depleted charged modifications
June 11, 2025 at 9:52 PM
We checked with untargeted SUGA to be sure (negative mode only), but only saw the more simple structures compared to what you’ve reported.
There’s a chance there was further processing involved of the spike protein by the manufacturer (like ion exchange) which may have depleted charged modifications
There’s a chance there was further processing involved of the spike protein by the manufacturer (like ion exchange) which may have depleted charged modifications