Alejandro Brenes
@ajbrenes.com
PostDoc @walmsley-lab.bsky.social @edinuni-irr.bsky.social @edinburgh-uni.bsky.social
| neutrophils single cell proteomics | road cyclist and aero bike enthusiast 🇨🇷🏴
Creator of the Immunological Proteome Resource @immpres.bsky.social
| neutrophils single cell proteomics | road cyclist and aero bike enthusiast 🇨🇷🏴
Creator of the Immunological Proteome Resource @immpres.bsky.social
Exciting to have @erinbakerims.bsky.social open day 2 of BMSS getting us to think about chemical exposure #BMSS2025
September 9, 2025 at 8:08 AM
Exciting to have @erinbakerims.bsky.social open day 2 of BMSS getting us to think about chemical exposure #BMSS2025
@nabalabuic.bsky.social opening up the Uncovering the Matrisome BSMB conference at Surrey.
September 2, 2025 at 12:19 PM
@nabalabuic.bsky.social opening up the Uncovering the Matrisome BSMB conference at Surrey.
As it's the wrap up European Symposium on Single Cell Proteomics we got to see how much it's grown with participants from Brazil and China! #ESCP2025
August 27, 2025 at 2:27 PM
As it's the wrap up European Symposium on Single Cell Proteomics we got to see how much it's grown with participants from Brazil and China! #ESCP2025
Mikaela now tells us about the scverse community intrageted ecosystem for single cell with established templates and data structures #ESCP2025
August 27, 2025 at 7:47 AM
Mikaela now tells us about the scverse community intrageted ecosystem for single cell with established templates and data structures #ESCP2025
Start of day 2 of the European Symposium on Single Cell Proteomics with @ryankellybyu.bsky.social telling us about high throughput proteomics towards a $10 proteome #ESCP2025
August 27, 2025 at 7:22 AM
Start of day 2 of the European Symposium on Single Cell Proteomics with @ryankellybyu.bsky.social telling us about high throughput proteomics towards a $10 proteome #ESCP2025
Alexander Ivanov talking about increases in sensitivity and signal to noise when changing to ultra low flor rates for single cell proteomics. #ESCP2025
August 26, 2025 at 3:43 PM
Alexander Ivanov talking about increases in sensitivity and signal to noise when changing to ultra low flor rates for single cell proteomics. #ESCP2025
The last session of the day is opened by Tami Geiger looking at the interactions of the immune cells with the cancer microenvironments using single cell proteomics #ESCP2025
August 26, 2025 at 3:02 PM
The last session of the day is opened by Tami Geiger looking at the interactions of the immune cells with the cancer microenvironments using single cell proteomics #ESCP2025
Next up is @pedroaragon.bsky.social talking about single cell and low input proteomics to study perturbation in leukemia #ESCP2025
August 26, 2025 at 12:47 PM
Next up is @pedroaragon.bsky.social talking about single cell and low input proteomics to study perturbation in leukemia #ESCP2025
Up next is @jwoessmann.bsky.social from the @erwinschoof.bsky.social lab showing how to focus on the exciting lower abundance proteins with single cell proteomics #ESCP2025
August 26, 2025 at 12:22 PM
Up next is @jwoessmann.bsky.social from the @erwinschoof.bsky.social lab showing how to focus on the exciting lower abundance proteins with single cell proteomics #ESCP2025
Daniela Valdes from @coscialab.bsky.social lab presents how smoking disrupts the placental function via macrophage dysregulation using single nuclei analysis and deep visual proteomics #ESCP2025
August 26, 2025 at 10:59 AM
Daniela Valdes from @coscialab.bsky.social lab presents how smoking disrupts the placental function via macrophage dysregulation using single nuclei analysis and deep visual proteomics #ESCP2025
Cool work from @nanopots.bsky.social doing cell surface profiling of 100-500 immine cells using affinity purification #ESCP2025
August 26, 2025 at 10:14 AM
Cool work from @nanopots.bsky.social doing cell surface profiling of 100-500 immine cells using affinity purification #ESCP2025
Next up is @vadim-demichev.bsky.social telling us how quantitatively accurate is single cell proteomics
#ESCP2025
#ESCP2025
August 26, 2025 at 8:19 AM
Next up is @vadim-demichev.bsky.social telling us how quantitatively accurate is single cell proteomics
#ESCP2025
#ESCP2025
After the DVP symposium now begins the start of the European Symposium on Single Cell Proteomics #ECSP25
A quick intro from @kmechtler.bsky.social and we kick off two days of exciting science starting with SCP of neutrophils in glioblastoma
A quick intro from @kmechtler.bsky.social and we kick off two days of exciting science starting with SCP of neutrophils in glioblastoma
August 26, 2025 at 6:59 AM
After the DVP symposium now begins the start of the European Symposium on Single Cell Proteomics #ECSP25
A quick intro from @kmechtler.bsky.social and we kick off two days of exciting science starting with SCP of neutrophils in glioblastoma
A quick intro from @kmechtler.bsky.social and we kick off two days of exciting science starting with SCP of neutrophils in glioblastoma
Start of the European symposium in single cell proteomics at beautiful Viena with a workshop on spatial proteomics organised by @coscialab.bsky.social
August 25, 2025 at 7:08 AM
Start of the European symposium in single cell proteomics at beautiful Viena with a workshop on spatial proteomics organised by @coscialab.bsky.social
In summary, we do single cell proteomics of glioblastoma neutrophils and find exciting functional states invisible to scRNAseq which further define the roles that neutrophils play within glioblastomas, showing some pro-tumorigenic and anti-tumorigenic populations and properties
July 27, 2025 at 12:41 PM
In summary, we do single cell proteomics of glioblastoma neutrophils and find exciting functional states invisible to scRNAseq which further define the roles that neutrophils play within glioblastomas, showing some pro-tumorigenic and anti-tumorigenic populations and properties
Finally we find a population with a lysosomal & protein degradation signature. They have high immunoglobulins, suggesting phagocytosis.
However this is not positive, as they have immunosuppresive (ARG1) & angiogenic signature. Perhaps phagocytosis reprograms to aid the tumour
However this is not positive, as they have immunosuppresive (ARG1) & angiogenic signature. Perhaps phagocytosis reprograms to aid the tumour
July 27, 2025 at 12:41 PM
Finally we find a population with a lysosomal & protein degradation signature. They have high immunoglobulins, suggesting phagocytosis.
However this is not positive, as they have immunosuppresive (ARG1) & angiogenic signature. Perhaps phagocytosis reprograms to aid the tumour
However this is not positive, as they have immunosuppresive (ARG1) & angiogenic signature. Perhaps phagocytosis reprograms to aid the tumour
Our lytic NET (LN) populations proteomic profile closely matches the @adrover.bsky.social vascular restricted population, with NETs actively causing occlusion of the vasculature leading to necrosis.
Our LN data suggests the same may occur in human GBM as we they show clotting and NETs signature
Our LN data suggests the same may occur in human GBM as we they show clotting and NETs signature
July 27, 2025 at 12:39 PM
Our lytic NET (LN) populations proteomic profile closely matches the @adrover.bsky.social vascular restricted population, with NETs actively causing occlusion of the vasculature leading to necrosis.
Our LN data suggests the same may occur in human GBM as we they show clotting and NETs signature
Our LN data suggests the same may occur in human GBM as we they show clotting and NETs signature
a lytic NETs population also looked vascular restricted. The proteome showed:
⬆️ coagulation & complement proteins
~ 90% ⬇️ in histones, azurophilic granules, secretory vesicles, nuclear membrane
Also had ⬇️ in metabolic & cell surface proteins, suggesting they can't respond to much anymore
⬆️ coagulation & complement proteins
~ 90% ⬇️ in histones, azurophilic granules, secretory vesicles, nuclear membrane
Also had ⬇️ in metabolic & cell surface proteins, suggesting they can't respond to much anymore
July 27, 2025 at 12:38 PM
a lytic NETs population also looked vascular restricted. The proteome showed:
⬆️ coagulation & complement proteins
~ 90% ⬇️ in histones, azurophilic granules, secretory vesicles, nuclear membrane
Also had ⬇️ in metabolic & cell surface proteins, suggesting they can't respond to much anymore
⬆️ coagulation & complement proteins
~ 90% ⬇️ in histones, azurophilic granules, secretory vesicles, nuclear membrane
Also had ⬇️ in metabolic & cell surface proteins, suggesting they can't respond to much anymore
two populations looked vascular restricted. The first shared the immature signature identified from the mini-bulk:
⬆️ mitochondrial & ribosomal proteins
⬆️ PCNA & CEBPE
⬇️ NADPH oxidase & CD16
The important of ECM proteins, this population had a blood signature with coagulation & apolipoproteins
⬆️ mitochondrial & ribosomal proteins
⬆️ PCNA & CEBPE
⬇️ NADPH oxidase & CD16
The important of ECM proteins, this population had a blood signature with coagulation & apolipoproteins
July 27, 2025 at 12:36 PM
two populations looked vascular restricted. The first shared the immature signature identified from the mini-bulk:
⬆️ mitochondrial & ribosomal proteins
⬆️ PCNA & CEBPE
⬇️ NADPH oxidase & CD16
The important of ECM proteins, this population had a blood signature with coagulation & apolipoproteins
⬆️ mitochondrial & ribosomal proteins
⬆️ PCNA & CEBPE
⬇️ NADPH oxidase & CD16
The important of ECM proteins, this population had a blood signature with coagulation & apolipoproteins
the vital NETs population also has ⬇️ granules, but combined with ⬇️ in almost all histones and nuclear membrane proteins. This decrease was not mirrored in the exhausted population.
July 27, 2025 at 12:36 PM
the vital NETs population also has ⬇️ granules, but combined with ⬇️ in almost all histones and nuclear membrane proteins. This decrease was not mirrored in the exhausted population.
data shows a trajectory where the exhausted neutrophils have degranulated without any hint of NETosis (histones are stable). But they do show marked ⬇️ in key metabolic enzymes in glycolysis, glycogenolysis and PPP.
They also have ⬇️ reg. of neutrophil functions like RAC2 and calmodulin
They also have ⬇️ reg. of neutrophil functions like RAC2 and calmodulin
July 27, 2025 at 12:35 PM
data shows a trajectory where the exhausted neutrophils have degranulated without any hint of NETosis (histones are stable). But they do show marked ⬇️ in key metabolic enzymes in glycolysis, glycogenolysis and PPP.
They also have ⬇️ reg. of neutrophil functions like RAC2 and calmodulin
They also have ⬇️ reg. of neutrophil functions like RAC2 and calmodulin
The engaged then seem to follow a trajectory that leads to exhaustion or vital NETosis.
just as a reminder neutrophil extracellular traps are mostly composed of DNA, histones and granule proteins. All invisible to RNAseq in mature neutrophils, but very much visible to proteomics
just as a reminder neutrophil extracellular traps are mostly composed of DNA, histones and granule proteins. All invisible to RNAseq in mature neutrophils, but very much visible to proteomics
July 27, 2025 at 12:35 PM
The engaged then seem to follow a trajectory that leads to exhaustion or vital NETosis.
just as a reminder neutrophil extracellular traps are mostly composed of DNA, histones and granule proteins. All invisible to RNAseq in mature neutrophils, but very much visible to proteomics
just as a reminder neutrophil extracellular traps are mostly composed of DNA, histones and granule proteins. All invisible to RNAseq in mature neutrophils, but very much visible to proteomics
The armed population had the highest abundance of migration related proteins, combined with highest abundance of granule proteins.
From there we believe they become engaged, so they start to release their granule content and reduce the abundance of some of the migration proteins.
From there we believe they become engaged, so they start to release their granule content and reduce the abundance of some of the migration proteins.
July 27, 2025 at 12:34 PM
The armed population had the highest abundance of migration related proteins, combined with highest abundance of granule proteins.
From there we believe they become engaged, so they start to release their granule content and reduce the abundance of some of the migration proteins.
From there we believe they become engaged, so they start to release their granule content and reduce the abundance of some of the migration proteins.