We analyzed development of activity over time across the different cell lines. The dissociated organoids had unsynchronized activity to begin with, but firing became synchronized across electrodes over time, and this happened across different cell lines. [Warning - video has flashing imagery.] 3/5

We grew unguided human neural organoids from four different ESC and iPSC lines and then dissociated them to 2D cultures that we plated onto micro electrode plates. We then recorded activity over time to characterize these networks and how that vary between lines and batches. 2/5