I believe that the current framework is more than sufficient, brain organoids are nowhere near the complexity that would require extensive regulation anytime soon. I think involving the lay public with poor understanding in regulating iPSC work would lead to unnecessary restrictions on research.

I think it’s back up now!

Cool! Have been playing around with it. Definitely like the protocols.io integration, it has a lot of potential even just for formattting protocols. I think the default auto conversion method uses chatGPT at the moment.

“Almost” zero hallucinations claimed - what does “almost” mean here? Any stats?

Congrats!

Hi Monica, would love to be added! I’m a Postdoc that works with brain organoids to study neurodevelopmental conditions and sex differences.
ORCID: 0000-0002-0138-8321
Research gate: www.researchgate.net/profile/Adam...

Thanks!

Thanks Rose! Making figures is my favorite part of paper writing 😁

Dissociated organoids have the benefit of initial 3D development as organoids and have more diverse cell composition compared to monocultures. We think this scalable approach could be applied to high-throughput studies of electrophysiological development! Go check out the preprint! 5/5

We did an in depth-characterization of functional connectivity in these networks using existing analysis tools and also used dimensionality reduction to integrate data across multiple MEA parameters, which can be great for visualizing the effects of treatments or comparing different conditions. 4/5

We analyzed development of activity over time across the different cell lines. The dissociated organoids had unsynchronized activity to begin with, but firing became synchronized across electrodes over time, and this happened across different cell lines. [Warning - video has flashing imagery.] 3/5

We grew unguided human neural organoids from four different ESC and iPSC lines and then dissociated them to 2D cultures that we plated onto micro electrode plates. We then recorded activity over time to characterize these networks and how that vary between lines and batches. 2/5