Thank you!

Thanks!

Archaeal S-layer proteins can adopt different symmetries that can locally accommodate areas of high curvature. The S-layer is also involved in the control of turgor and osmotic pressure, ion transport, cell-cell interactions and mating.

The S-layer is the most widespread and fundamental component of archaeal cell envelopes and may represent one of the earliest and most fundamental cell wall polymers found in microorganisms

To explore how ring assembly and DNA segregation might be coupled, we turned our attention to the ParA homologue, SegA, and its partner SegB, using antibodies kindly gifted by @acharlesorszag.bsky.social (see www.biorxiv.org/content/10.1...)

Oop!

If I may weigh in here: I avoid overfixing the cells, especially if using glutaraldehyde that can induce significant artefacts. I usually do 30-60 min at RT and rinse abundantly in buffer. Fixed cells in buffer can then be kept for days in the fridge :)

Hahaha awww 🫶🏼 I’m very ignorant when it comes to anaerobic cultures. What are the challenging steps pre-fixation? The spinning?

6) in all subsequent rinsing and post-fixation steps, avoid drying the cells as much as possible. We never discard the full volume of liquid in the wells. Instead, we only discard 80-90% but make up for it by performing more rinsing steps

5) add an equal volume of a 2X fixative solution in adequate buffer, or ideally in culture media, as opposed to removing the media and then adding 1X fixative to the cells

4) spin the plate containing the cells/coverslips (24-well plate, 13 mm coverslips in our hands) at 1,000xg for 2 min to increase cell density on the coverslip