Abby Buchwalter
@abbybuch.bsky.social
Associate professor at UCSF. Cell biologist at heart, research focus on the function and dynamic regulation of the nuclear periphery.
www.buchwalterlab.UCSF.edu.
www.buchwalterlab.UCSF.edu.
Personal good news alert: I’ve been promoted to associate professor and it’s time to update the ol’ bio! Launching my lab at UCSF has been a privilege + I’m so grateful to my team and community here. More exciting science ahead! 🚀
November 6, 2025 at 8:12 PM
Personal good news alert: I’ve been promoted to associate professor and it’s time to update the ol’ bio! Launching my lab at UCSF has been a privilege + I’m so grateful to my team and community here. More exciting science ahead! 🚀
Apple picking with the crew
October 21, 2025 at 7:00 PM
Apple picking with the crew
OK, an NIH grant question that I would normally ask my PO but can't because of shutdown 🫠...
Interested in applying for NIGMS RM1 team grant with colleagues. Several of us are NIGMS-funded. Are we eligible? Funds for lab but not our salary, or no more GM funds for us, full stop? (see screenshot)
Interested in applying for NIGMS RM1 team grant with colleagues. Several of us are NIGMS-funded. Are we eligible? Funds for lab but not our salary, or no more GM funds for us, full stop? (see screenshot)
October 13, 2025 at 4:36 PM
OK, an NIH grant question that I would normally ask my PO but can't because of shutdown 🫠...
Interested in applying for NIGMS RM1 team grant with colleagues. Several of us are NIGMS-funded. Are we eligible? Funds for lab but not our salary, or no more GM funds for us, full stop? (see screenshot)
Interested in applying for NIGMS RM1 team grant with colleagues. Several of us are NIGMS-funded. Are we eligible? Funds for lab but not our salary, or no more GM funds for us, full stop? (see screenshot)
Weekend reset in the Sierras - Lake Schmidell, Desolation Wilderness.
July 29, 2025 at 5:29 PM
Weekend reset in the Sierras - Lake Schmidell, Desolation Wilderness.
Ok...we still have live stem cells, so does this matter? We pushed our mutants to differentiate from "naive" into "primed" pluripotency. Our mutants can't make this transition. Instead, they die. (7/n)
July 22, 2025 at 5:19 PM
Ok...we still have live stem cells, so does this matter? We pushed our mutants to differentiate from "naive" into "primed" pluripotency. Our mutants can't make this transition. Instead, they die. (7/n)
Yup! When displaced into the nucleus, H3K9me2 allows more transcription - including of parasitic mobile elements (transposons) that are normally repressed to maintain genome integrity. Location does matter: the nuclear periphery enhances heterochromatin's repressive capacity. (6/n)
July 22, 2025 at 5:18 PM
Yup! When displaced into the nucleus, H3K9me2 allows more transcription - including of parasitic mobile elements (transposons) that are normally repressed to maintain genome integrity. Location does matter: the nuclear periphery enhances heterochromatin's repressive capacity. (6/n)
In stem cells, the H3K9me2 modification is enriched on heterochromatin at the nuclear periphery. When we ablated the proteins that tether it, H3K9me2 remained on the same genes/loci in spite of its movement away from the periphery. (4/n)
July 22, 2025 at 5:16 PM
In stem cells, the H3K9me2 modification is enriched on heterochromatin at the nuclear periphery. When we ablated the proteins that tether it, H3K9me2 remained on the same genes/loci in spite of its movement away from the periphery. (4/n)
We did this by removing four genes in stem cells: all 3 lamins + the nuclear membrane protein LBR. Aha! Heterochromatin positions are reshuffled. (3/n)
July 22, 2025 at 5:15 PM
We did this by removing four genes in stem cells: all 3 lamins + the nuclear membrane protein LBR. Aha! Heterochromatin positions are reshuffled. (3/n)
And yep - we found that the nuclear membrane protein LAP2b cooperates with lamin B1 to maintain nuclear integrity. When both are depleted, we see lamina defects and nuclear ruptures that look like this (cyan is nuclear lamina, magenta is nuclear integrity reporter): (5/n)
July 21, 2025 at 5:49 PM
And yep - we found that the nuclear membrane protein LAP2b cooperates with lamin B1 to maintain nuclear integrity. When both are depleted, we see lamina defects and nuclear ruptures that look like this (cyan is nuclear lamina, magenta is nuclear integrity reporter): (5/n)
Congrats to Dr. Yewande Alabi, Ph.D.!
May 16, 2025 at 8:20 PM
Congrats to Dr. Yewande Alabi, Ph.D.!
Friday office flowers to brighten your timeline
April 25, 2025 at 10:38 PM
Friday office flowers to brighten your timeline
Fuel for lab cleanup day 💪 💪
March 13, 2025 at 3:45 PM
Fuel for lab cleanup day 💪 💪
Heading to my SF local #standupforscience rally tomorrow along with many UCSF colleagues and friends. Making signs this afternoon at departmental happy hour hosted by @elphegenoralab.bsky.social - we need inspiration!
March 6, 2025 at 8:00 PM
Heading to my SF local #standupforscience rally tomorrow along with many UCSF colleagues and friends. Making signs this afternoon at departmental happy hour hosted by @elphegenoralab.bsky.social - we need inspiration!
A little timeline cleanse: literally stopped to smell the roses on my way to work this AM. Sunny morning was calling me to walk in, with a stop at the SF flower market to brighten up my office. (Ranunculus, my fave flowers, were the winners.) some wandering labware as vase..
February 26, 2025 at 6:33 PM
A little timeline cleanse: literally stopped to smell the roses on my way to work this AM. Sunny morning was calling me to walk in, with a stop at the SF flower market to brighten up my office. (Ranunculus, my fave flowers, were the winners.) some wandering labware as vase..
Ahh, the soothing familiarity of running a BCA assay on our ancient plate reader. I’m still sciencing, Elon! You can’t take that away from me 🧪
February 25, 2025 at 12:48 AM
Ahh, the soothing familiarity of running a BCA assay on our ancient plate reader. I’m still sciencing, Elon! You can’t take that away from me 🧪
Things that are making me smile today: Disco the gym dog
February 9, 2025 at 7:54 PM
Things that are making me smile today: Disco the gym dog
And Jessica Mella, also on Tuesday!
December 15, 2024 at 5:53 PM
And Jessica Mella, also on Tuesday!
Come see Charlie Allen on Tuesday!
December 15, 2024 at 5:48 PM
Come see Charlie Allen on Tuesday!
First up, Tracy Knight!
December 15, 2024 at 5:47 PM
First up, Tracy Knight!
Cruising into #cellbio2024 with lab members and friends! Looking forward to a week of connecting with new friends and old. Come check out what our team is up to at posters today and Tuesday.
December 15, 2024 at 5:44 PM
Cruising into #cellbio2024 with lab members and friends! Looking forward to a week of connecting with new friends and old. Come check out what our team is up to at posters today and Tuesday.
Let’s go! Feels hopeful to be out here helping my neighbors vote.
November 5, 2024 at 10:03 PM
Let’s go! Feels hopeful to be out here helping my neighbors vote.