Charles Winterhalter
@winterhalterlab.bsky.social
Wellcome Early-Career Fellow investigating novel DNA damage repair mechanisms in bacteria at Newcastle university (UK)
Pinned
A cool genetic system to study site-pecific replication fork collapse and repair from the brilliant @winterhalterlab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading - Nature Communications
DNA damage can lead to cell death. Here, the authors show that a simple cut on either strand of DNA can inactivate bacterial chromosome replication. Surprisingly, only a core set of recombination prot...
www.nature.com
Recombinational repair is the major pathway required to reload the bacterial replicative helicase following introduction of single-strand DNA breaks. A great way to end the year! Big thank you to co-authors from the @heathmurraylab.bsky.social
Recombinational repair is the major pathway required to reload the bacterial replicative helicase following introduction of single-strand DNA breaks. A great way to end the year! Big thank you to co-authors from the @heathmurraylab.bsky.social
A cool genetic system to study site-pecific replication fork collapse and repair from the brilliant @winterhalterlab.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Rescuing the bacterial replisome at a nick requires recombinational repair and helicase reloading - Nature Communications
DNA damage can lead to cell death. Here, the authors show that a simple cut on either strand of DNA can inactivate bacterial chromosome replication. Surprisingly, only a core set of recombination prot...
www.nature.com
December 30, 2025 at 6:31 PM
Recombinational repair is the major pathway required to reload the bacterial replicative helicase following introduction of single-strand DNA breaks. A great way to end the year! Big thank you to co-authors from the @heathmurraylab.bsky.social
Congratulations to Hannah and colleagues for depicting the consequences of DNA replication hyper-initiation in Gram-positive bacteria! @microbiologysociety.org www.microbiologyresearch.org/content/jour...
Conflicts between the DNA replication and repair machineries promote cell death in Gram-positive bacteria
Cellular proliferation relies on the successful coordination and completion of genome replication and segregation. To help achieve this, many bacteria utilize regulatory pathways that ensure DNA repli...
www.microbiologyresearch.org
December 30, 2025 at 6:18 PM
Congratulations to Hannah and colleagues for depicting the consequences of DNA replication hyper-initiation in Gram-positive bacteria! @microbiologysociety.org www.microbiologyresearch.org/content/jour...
Reposted by Charles Winterhalter
New cryo-EM/ET story - a brilliant collaboration with Sergey Melnikov's lab in Newcastle @sergeymelnikov.bsky.social and Stefan Pfeffer's lab in Heidelberg @pfeffercryolab.bsky.social. Great work in particular by Karla Helena-Bueno and @sophiekopetschke.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structurally heterogeneous ribosomes cooperate in protein synthesis in bacterial cells - Nature Communications
Cells can simultaneously produce structurally dissimilar ribosomes, suggesting functional specialization of distinct ribosome populations. Here, the authors show that distinct ribosomes cooperate rath...
www.nature.com
March 21, 2025 at 9:30 AM
New cryo-EM/ET story - a brilliant collaboration with Sergey Melnikov's lab in Newcastle @sergeymelnikov.bsky.social and Stefan Pfeffer's lab in Heidelberg @pfeffercryolab.bsky.social. Great work in particular by Karla Helena-Bueno and @sophiekopetschke.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Still time to apply and join my team on a fully funded PhD project looking at dynamics of the bacterial DNA damage response in live cells using genomics.
Engineering anti-CRISPR/Cas systems to control the DNA damage response in live bacterial cells. Supervised by @winterhalterlab.bsky.social, @acdarby.bsky.social, and Katarzyna Mickiewicz.
www.findaphd.com/phds/project...
www.findaphd.com/phds/project...
Engineering anti-CRISPR/Cas systems to control the DNA damage response in live bacterial cells at Newcastle University on FindAPhD.com
PhD Project - Engineering anti-CRISPR/Cas systems to control the DNA damage response in live bacterial cells at Newcastle University, listed on FindAPhD.com
www.findaphd.com
March 20, 2025 at 9:50 AM
Still time to apply and join my team on a fully funded PhD project looking at dynamics of the bacterial DNA damage response in live cells using genomics.
Reposted by Charles Winterhalter
This was a fantastic collaboration with our neighbors @mblokesch.bsky.social & David Adams and Mark Szczelkun and @steven-shaw.bsky.social in Bristol. Special thanks to the DCI for invaluable support with cryo-EM of a rather difficult target! @drhonsworth.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Structure and Activation Mechanism of a Lamassu Phage Defence System
Lamassu is a diverse family of defence systems that protect bacteria, including pandemic strains of Vibrio cholerae, against phage infection. They target essential cellular processes, aborting infecti...
www.biorxiv.org
March 16, 2025 at 9:18 AM
This was a fantastic collaboration with our neighbors @mblokesch.bsky.social & David Adams and Mark Szczelkun and @steven-shaw.bsky.social in Bristol. Special thanks to the DCI for invaluable support with cryo-EM of a rather difficult target! @drhonsworth.bsky.social
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Beautiful work from the Dewar lab and colleagues diving into replication fork rescue in vertebrates. Together, our papers highlight critical similarities/differences in DNA damage repair processes occurring in prokaryotes vs eukaryotes when the replisome encounters a single-strand discontinuity.
🧬⛓️ Our latest preprint examines the fate of collapsed forks in vertebrates 🐸 and our colleagues in @heathmurraylab.bsky.social asked similar questions in bacteria 🦠 to reveal how evolution shapes the response to fork collapse (see🧵)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Resolution of collapsed forks is separate from completion of DNA synthesis
Replication fork collapse at single-strand DNA breaks (SSBs) poses a serious threat to genome stability. Using Xenopus egg extracts, we show that a replication fork encountering an SSB on either the l...
www.biorxiv.org
March 11, 2025 at 12:13 PM
Beautiful work from the Dewar lab and colleagues diving into replication fork rescue in vertebrates. Together, our papers highlight critical similarities/differences in DNA damage repair processes occurring in prokaryotes vs eukaryotes when the replisome encounters a single-strand discontinuity.
Excited to share with you the story that enabled me to start my own lab: what happens when the bacterial DNA replication machinery encounters single-strand discontinuities on template DNA? What is required for break repair and replication restart?
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Rescuing bacterial genome replication: essential functions to repair a double-strand break and restart DNA synthesis
Many antibiotics indirectly generate reactive oxygen species (ROS) that can damage bacterial genomes. Oxidised nucleobases become genotoxic when they are targeted for repair through excision, generati...
www.biorxiv.org
March 11, 2025 at 12:03 PM
Excited to share with you the story that enabled me to start my own lab: what happens when the bacterial DNA replication machinery encounters single-strand discontinuities on template DNA? What is required for break repair and replication restart?
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...