William Pearman
@williampearman.bsky.social
Research fellow at Auckland Uni. Wannabe bioinformatician, reluctant community ecologist. Evolutionary ecology, microbial ecology. Anglican. He/him
Anyone on here ever tried using a laser to break open really tough samples for DNA extraction? I'm working on some tough chitinous tissues which nothing seems to reliably lyse them - and thought I might take some inspiration from laser microdissection (which is a tad overkill/$$)
June 12, 2025 at 8:43 AM
Anyone on here ever tried using a laser to break open really tough samples for DNA extraction? I'm working on some tough chitinous tissues which nothing seems to reliably lyse them - and thought I might take some inspiration from laser microdissection (which is a tad overkill/$$)
Has anyone here got much experience with microbial ecology and experimental pre-registration? I'm embarking on a new project, and would like to pre-register & peer review the experimental design - but I have no idea where to start.
February 25, 2025 at 8:39 PM
Has anyone here got much experience with microbial ecology and experimental pre-registration? I'm embarking on a new project, and would like to pre-register & peer review the experimental design - but I have no idea where to start.
Reposted by William Pearman
🚨 Free one-week in person workshop on SLiM from 24th - 28th March at the University of Auckland. 📉🧬
Hands on tutorials will be lead by experienced SLiM users Dr @williampearman.bsky.social and Anna Clark.
Some ECR travel funds available.🎉
Register here 👉 docs.google.com/forms/d/e/1F...
Hands on tutorials will be lead by experienced SLiM users Dr @williampearman.bsky.social and Anna Clark.
Some ECR travel funds available.🎉
Register here 👉 docs.google.com/forms/d/e/1F...
docs.google.com
January 27, 2025 at 11:30 PM
🚨 Free one-week in person workshop on SLiM from 24th - 28th March at the University of Auckland. 📉🧬
Hands on tutorials will be lead by experienced SLiM users Dr @williampearman.bsky.social and Anna Clark.
Some ECR travel funds available.🎉
Register here 👉 docs.google.com/forms/d/e/1F...
Hands on tutorials will be lead by experienced SLiM users Dr @williampearman.bsky.social and Anna Clark.
Some ECR travel funds available.🎉
Register here 👉 docs.google.com/forms/d/e/1F...
Very glad to have this paper out - one of the cooler things to come out of my PhD. This also happens to be (likely) the last publication to come out of my PhD as well!
Host dispersal relaxes selective pressures in rafting microbiomes and triggers successional changes - Nature Communications
The microbiomes of dispersing organisms are understudied. Here, the authors examine rafting seaweed microbiomes, using oceanographic modelling to demonstrate significant community changes associated w...
www.nature.com
January 2, 2025 at 1:56 AM
Very glad to have this paper out - one of the cooler things to come out of my PhD. This also happens to be (likely) the last publication to come out of my PhD as well!
I don't understand why people don't sequence negative extraction controls. I've spent more time recently managing reagent contamination for low biomass extractions than i've spent doing the actual extractions. Extensive sterile filtering, UV, and EMA treatment seemed to be the only way that worked.
relevant to a paper I just reviewed (and cited this paper bmcbiol.biomedcentral.com/articles/10....). Ppl seem resistant to this lesson...
Reagent and laboratory contamination can critically impact sequence-based microbiome analyses - BMC Biology
Background The study of microbial communities has been revolutionised in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metag...
bmcbiol.biomedcentral.com
December 20, 2024 at 7:56 PM
I don't understand why people don't sequence negative extraction controls. I've spent more time recently managing reagent contamination for low biomass extractions than i've spent doing the actual extractions. Extensive sterile filtering, UV, and EMA treatment seemed to be the only way that worked.
Reposted by William Pearman
Am I right in assuming that blunting our humanities and social sciences research will dull our understanding of inequities suffered by Māori, and the role that a just Te Tiriti interpretation would play in resolving this?
Because if so, it's hard not to connect the dots to the true intent here.
Because if so, it's hard not to connect the dots to the true intent here.
"The focus of the Fund will shift to core science, with the humanities and social sciences panels disbanded and no longer supported."
The changes are bad for science, they're basically fatal for meaningful govt support of humanities and social sciences. Blatant and unashamed anti-intellectualism.
The changes are bad for science, they're basically fatal for meaningful govt support of humanities and social sciences. Blatant and unashamed anti-intellectualism.
I do not yet have a comment on this but I ask all New Zealand researchers and businesses read this and consider the full impact of this signal #nzpol www.beehive.govt.nz/release/mars...
December 4, 2024 at 1:40 AM
Am I right in assuming that blunting our humanities and social sciences research will dull our understanding of inequities suffered by Māori, and the role that a just Te Tiriti interpretation would play in resolving this?
Because if so, it's hard not to connect the dots to the true intent here.
Because if so, it's hard not to connect the dots to the true intent here.
Thrilled to have this preprint out today and to have been awarded a Mana Tūāpapa Future Leader Fellowship to work on Daphnia microbiomes for the next 4 years!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Joint consideration of selection and microbial generation count provides unique insights into evolutionary and ecological dynamics of holobionts
The relationship between, and joint selection on, a host and its microbes - the holobiont - can impact evolutionary and ecological outcomes of the host and its microbial community. Here we present a n...
www.biorxiv.org
November 14, 2024 at 4:02 AM
Thrilled to have this preprint out today and to have been awarded a Mana Tūāpapa Future Leader Fellowship to work on Daphnia microbiomes for the next 4 years!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...