UConn MARS Facility 🧪🧫🦠
@uconnmars.bsky.social
Microbiome and amplicon focused sequencing core lab. Home to @dnakendra and UConn Wastewater Analysis Center.
https://mars.uconn.edu mars@uconn.edu
https://mars.uconn.edu mars@uconn.edu
MARS is now offering longreads! Come to ESB lobby at noon tomorrow to hear about our nanopore applications.
April 24, 2024 at 1:43 PM
MARS is now offering longreads! Come to ESB lobby at noon tomorrow to hear about our nanopore applications.
Reposted by UConn MARS Facility 🧪🧫🦠
Next week! UConn MCB microbiome symposium. Registration is still open. mcb.uconn.edu/microbiology...
Microbiology Symposium | Department of Molecular and Cell Biology
8th Annual Distinguished Microbiologist Lecture and Microbiology SymposiumThe Microbiology Symposium provides a critical hub around which UConn microbiologi ...
mcb.uconn.edu
April 1, 2024 at 2:31 PM
Next week! UConn MCB microbiome symposium. Registration is still open. mcb.uconn.edu/microbiology...
Reposted by UConn MARS Facility 🧪🧫🦠
🦠🧪I've had a student from a Nigerian university contact me for 16s sequencing. She's naturally concerned about cost including the exchange rate and shipping. I'd love to be able to send her contact info for a Nigerian (or at least African) sequencing that can do 16s for her. Suggestions?
March 28, 2024 at 4:50 PM
🦠🧪I've had a student from a Nigerian university contact me for 16s sequencing. She's naturally concerned about cost including the exchange rate and shipping. I'd love to be able to send her contact info for a Nigerian (or at least African) sequencing that can do 16s for her. Suggestions?
Reposted by UConn MARS Facility 🧪🧫🦠
Another workshop on the books. My brain is just as squishy at the end as always. 4 packed days of mothur and R.
@uconnmars.bsky.social
@uconnmars.bsky.social
March 14, 2024 at 8:32 PM
Another workshop on the books. My brain is just as squishy at the end as always. 4 packed days of mothur and R.
@uconnmars.bsky.social
@uconnmars.bsky.social
Reposted by UConn MARS Facility 🧪🧫🦠
I have truseq libraries from phenol:chloroform extracted bacteria. Test run way underclustered (top 180k, bottom 500k) so i think I may have carry over pci. Illumina tech support said start over, no way to clean. But that can't be right??
I have beads, pcr cleanup kits, and amicon. What to use?
🧪🦠
I have beads, pcr cleanup kits, and amicon. What to use?
🧪🦠
March 18, 2024 at 4:25 PM
I have truseq libraries from phenol:chloroform extracted bacteria. Test run way underclustered (top 180k, bottom 500k) so i think I may have carry over pci. Illumina tech support said start over, no way to clean. But that can't be right??
I have beads, pcr cleanup kits, and amicon. What to use?
🧪🦠
I have beads, pcr cleanup kits, and amicon. What to use?
🧪🦠
Reposted by UConn MARS Facility 🧪🧫🦠
Last call for my intro to microbial ecology workshop. No command line experience is needed. I'll show you how to use bash and mothur for sequence qc and otu clustering, then we move to ecological statistics in R. Next week mars.uconn.edu/workshops/
Workshops | Microbial Analysis, Resources, and Services
Thanks for your interest in MARS workshops! Upcoming Workshops: Microbiome and Community analysis - from sequences to figures, Aug 14 -17, 2023. In 4 days w ...
mars.uconn.edu
March 7, 2024 at 12:24 PM
Last call for my intro to microbial ecology workshop. No command line experience is needed. I'll show you how to use bash and mothur for sequence qc and otu clustering, then we move to ecological statistics in R. Next week mars.uconn.edu/workshops/
Reposted by UConn MARS Facility 🧪🧫🦠
Towards estimating the number of strains that make up a natural bacterial population. doi.org/10.1038/s414...
Towards estimating the number of strains that make up a natural bacterial population - Nature Commun...
What a microbial strain is and how many strains make up a natural bacterial population remain elusive concepts. Here, Viver et al. analyse Salinibacter ruber isolates and metagenomes from two solar sa...
doi.org
January 19, 2024 at 7:44 AM
Towards estimating the number of strains that make up a natural bacterial population. doi.org/10.1038/s414...
Reposted by UConn MARS Facility 🧪🧫🦠
@cbcuconn.bsky.social is offering 2 virtual #bioinformatics training workshops in Jan! Genome Assembly (Jan 8-11) and Genome Annotation (Jan 22-25). 1/2 days, real instructors, real data examples! Learn more & register here: bioinformatics.uconn.edu/cbc-workshops/
January 4, 2024 at 12:02 AM
@cbcuconn.bsky.social is offering 2 virtual #bioinformatics training workshops in Jan! Genome Assembly (Jan 8-11) and Genome Annotation (Jan 22-25). 1/2 days, real instructors, real data examples! Learn more & register here: bioinformatics.uconn.edu/cbc-workshops/
Reposted by UConn MARS Facility 🧪🧫🦠
Wonderful BlueSky:
We are looking for graduate students and postdocs who could benefit from intense exposure to all-things-microbial at the 2024 Microbial Diversity Course at the Marine Biological Laboratory in Woods Hole, MA.
Consider applying / reposting.
www.mbl.edu/education/ad...
We are looking for graduate students and postdocs who could benefit from intense exposure to all-things-microbial at the 2024 Microbial Diversity Course at the Marine Biological Laboratory in Woods Hole, MA.
Consider applying / reposting.
www.mbl.edu/education/ad...
December 28, 2023 at 12:22 AM
Wonderful BlueSky:
We are looking for graduate students and postdocs who could benefit from intense exposure to all-things-microbial at the 2024 Microbial Diversity Course at the Marine Biological Laboratory in Woods Hole, MA.
Consider applying / reposting.
www.mbl.edu/education/ad...
We are looking for graduate students and postdocs who could benefit from intense exposure to all-things-microbial at the 2024 Microbial Diversity Course at the Marine Biological Laboratory in Woods Hole, MA.
Consider applying / reposting.
www.mbl.edu/education/ad...
Reposted by UConn MARS Facility 🧪🧫🦠
MARS is a certified Nanopore sequencing provider! Contact us now for any standard libray preps.
Stay tuned for details about our custom 16S/18S/ITS/CO1 long amplicons. We'll be offering this service on a per sample basis-no need to fill a flow cell yourself. Prelim data and pricing in Jan!
Stay tuned for details about our custom 16S/18S/ITS/CO1 long amplicons. We'll be offering this service on a per sample basis-no need to fill a flow cell yourself. Prelim data and pricing in Jan!
December 16, 2023 at 1:11 AM
MARS is a certified Nanopore sequencing provider! Contact us now for any standard libray preps.
Stay tuned for details about our custom 16S/18S/ITS/CO1 long amplicons. We'll be offering this service on a per sample basis-no need to fill a flow cell yourself. Prelim data and pricing in Jan!
Stay tuned for details about our custom 16S/18S/ITS/CO1 long amplicons. We'll be offering this service on a per sample basis-no need to fill a flow cell yourself. Prelim data and pricing in Jan!
MARS is a certified Nanopore sequencing provider! Contact us now for any standard libray preps.
Stay tuned for details about our custom 16S/18S/ITS/CO1 long amplicons. We'll be offering this service on a per sample basis-no need to fill a flow cell yourself. Prelim data and pricing in Jan!
Stay tuned for details about our custom 16S/18S/ITS/CO1 long amplicons. We'll be offering this service on a per sample basis-no need to fill a flow cell yourself. Prelim data and pricing in Jan!
December 16, 2023 at 1:11 AM
MARS is a certified Nanopore sequencing provider! Contact us now for any standard libray preps.
Stay tuned for details about our custom 16S/18S/ITS/CO1 long amplicons. We'll be offering this service on a per sample basis-no need to fill a flow cell yourself. Prelim data and pricing in Jan!
Stay tuned for details about our custom 16S/18S/ITS/CO1 long amplicons. We'll be offering this service on a per sample basis-no need to fill a flow cell yourself. Prelim data and pricing in Jan!
We can help you get into eDNA! Come talk to us about what you want to look for and where.
A nice explainer about environmental DNA from Maarten De Brauwer #eDNA
It sounds like science fiction. But we can now sample water to find the DNA of every species living ...
Every living thing leaves traces in its environment. By sampling water or even air for this environmental DNA, we can know which species live where.
theconversation.com
November 16, 2023 at 2:14 PM
We can help you get into eDNA! Come talk to us about what you want to look for and where.
Reposted by UConn MARS Facility 🧪🧫🦠
Periodic reminder that there is a feed for the reading list I post attached to #jcampubs focused on environmental microbiology with some marine science thrown in!
bsky.app/profile/did:...
bsky.app/profile/did:...
November 15, 2023 at 2:51 PM
Periodic reminder that there is a feed for the reading list I post attached to #jcampubs focused on environmental microbiology with some marine science thrown in!
bsky.app/profile/did:...
bsky.app/profile/did:...
Want to try looking at methylation in your bacteria?
Doing some benchmarking, you can very confidently identify methylation as low as 10x coverage - which is pretty crazy because that's actually ~5x (methylation is stranded)
November 15, 2023 at 3:29 PM
Want to try looking at methylation in your bacteria?
Reposted by UConn MARS Facility 🧪🧫🦠
Bacteria can maintain rRNA operons solely on plasmids for hundreds of millions of years www.nature.com/articles/s41... #jcampubs
Bacteria can maintain rRNA operons solely on plasmids for hundreds of millions of years - Nature Com...
Bacteria usually have at least one rRNA operon on the chromosome, suggesting that the exclusive presence of rRNA operons on a plasmid is rare and unlikely to be stably maintained. Here, Anda et al. fi...
www.nature.com
November 14, 2023 at 1:57 PM
Bacteria can maintain rRNA operons solely on plasmids for hundreds of millions of years www.nature.com/articles/s41... #jcampubs
If you want community structure, do 16s. If you want functional profiling, do (meta)transcriptomics.
This is more evidence that my refusal to help people run their (v4) 16s through a functional prediction tool was scientifically valid. Anyone using these tools should know their data and the tool.
This is more evidence that my refusal to help people run their (v4) 16s through a functional prediction tool was scientifically valid. Anyone using these tools should know their data and the tool.
On the limits of 16S rRNA gene-based metagenome prediction and functional profiling www.biorxiv.org/content/10.1... #jcampubs
On the limits of 16S rRNA gene-based metagenome prediction and functional profiling
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
November 9, 2023 at 2:21 PM
If you want community structure, do 16s. If you want functional profiling, do (meta)transcriptomics.
This is more evidence that my refusal to help people run their (v4) 16s through a functional prediction tool was scientifically valid. Anyone using these tools should know their data and the tool.
This is more evidence that my refusal to help people run their (v4) 16s through a functional prediction tool was scientifically valid. Anyone using these tools should know their data and the tool.
Happening soon! I'll be talking about MARS remotely because I didn't want to bring my central CT cold down to the shore. I'm still available for meetings this afternoon just via video chat.
@uconn.bsky.social Avery Point. @dnakendra.bsky.social will be on your fine campus next Wed (Nov 8) to give the Marine Science brownbag at noon.
Come see me talk. Let me know if you want to meet afterward. I'm planning on staying as late in the afternoon as people want to meet.
Come see me talk. Let me know if you want to meet afterward. I'm planning on staying as late in the afternoon as people want to meet.
November 8, 2023 at 4:31 PM
Happening soon! I'll be talking about MARS remotely because I didn't want to bring my central CT cold down to the shore. I'm still available for meetings this afternoon just via video chat.
Reposted by UConn MARS Facility 🧪🧫🦠
@uconn.bsky.social Avery Point. @dnakendra.bsky.social will be on your fine campus next Wed (Nov 8) to give the Marine Science brownbag at noon.
Come see me talk. Let me know if you want to meet afterward. I'm planning on staying as late in the afternoon as people want to meet.
Come see me talk. Let me know if you want to meet afterward. I'm planning on staying as late in the afternoon as people want to meet.
November 2, 2023 at 3:03 PM
@uconn.bsky.social Avery Point. @dnakendra.bsky.social will be on your fine campus next Wed (Nov 8) to give the Marine Science brownbag at noon.
Come see me talk. Let me know if you want to meet afterward. I'm planning on staying as late in the afternoon as people want to meet.
Come see me talk. Let me know if you want to meet afterward. I'm planning on staying as late in the afternoon as people want to meet.
@uconn.bsky.social Avery Point. @dnakendra.bsky.social will be on your fine campus next Wed (Nov 8) to give the Marine Science brownbag at noon.
Come see me talk. Let me know if you want to meet afterward. I'm planning on staying as late in the afternoon as people want to meet.
Come see me talk. Let me know if you want to meet afterward. I'm planning on staying as late in the afternoon as people want to meet.
November 2, 2023 at 3:03 PM
@uconn.bsky.social Avery Point. @dnakendra.bsky.social will be on your fine campus next Wed (Nov 8) to give the Marine Science brownbag at noon.
Come see me talk. Let me know if you want to meet afterward. I'm planning on staying as late in the afternoon as people want to meet.
Come see me talk. Let me know if you want to meet afterward. I'm planning on staying as late in the afternoon as people want to meet.
Reposted by UConn MARS Facility 🧪🧫🦠
More evidence of Impact Factor Mania journals.asm.org/doi/full/10....
More evidence of Impact Factor Mania | Microbiology Spectrum
Microbiology Spectrum was initially launched in 2011 to provide authors who were publishing monographs
with a PubMed indexed, citable publication. Because ASM made the strategic decision
over the past...
journals.asm.org
November 2, 2023 at 1:37 PM
More evidence of Impact Factor Mania journals.asm.org/doi/full/10....
@uconnscs.bsky.social wanna try it?
Ne paper! Want to predict ecological interactions? We argue that graph embedding techniques provide more appropriate data representations, that allow both in and out-of sample predictions.
📑 besjournals.onlinelibrary.wiley.com/doi/10.1111/...
🧑💻 poisotlab.github.io/ms_metaweb_p...
🧪
📑 besjournals.onlinelibrary.wiley.com/doi/10.1111/...
🧑💻 poisotlab.github.io/ms_metaweb_p...
🧪
October 19, 2023 at 1:17 PM
@uconnscs.bsky.social wanna try it?
Reposted by UConn MARS Facility 🧪🧫🦠
Phables: from fragmented assemblies to high-quality bacteriophage genomes academic.oup.com/bioinformati... #jcampubs
Phables: from fragmented assemblies to high-quality bacteriophage genomes
AbstractMotivation. Microbial communities have a profound impact on both human health and various environments. Viruses infecting bacteria, known as bacteriopha
academic.oup.com
October 17, 2023 at 1:12 PM
Phables: from fragmented assemblies to high-quality bacteriophage genomes academic.oup.com/bioinformati... #jcampubs
🦠🧫🧪
1.17B proteins from 26,931 metagenomes with no similarity to anything in reference genomes. Cluster into 106,198 novel clusters with more than 100 members and predict structures!
@sokrypton.org
www.nature.com/articles/s41...
@sokrypton.org
www.nature.com/articles/s41...
October 16, 2023 at 6:27 PM
🦠🧫🧪
MARS is here! Follow this account for facility updates and paper retweets. 🦠🧪
October 14, 2023 at 4:10 PM
Reposted by UConn MARS Facility 🧪🧫🦠
The littles have yet another cold, so we're having a quiet day watching volcano documentaries (4yo's current obsession). Found a cool one about axal seamont from opb, then it autoplayed this amazing piece on soil microbial ecology in space. 🧪🦠
watch.opb.org/video/space-...
watch.opb.org/video/space-...
All Science. No Fiction. | Space Dirt | Season 2 | Episode 3 | OPB
Soil microbes will be key to any successful long-term settlement in space.
watch.opb.org
October 7, 2023 at 5:08 PM
The littles have yet another cold, so we're having a quiet day watching volcano documentaries (4yo's current obsession). Found a cool one about axal seamont from opb, then it autoplayed this amazing piece on soil microbial ecology in space. 🧪🦠
watch.opb.org/video/space-...
watch.opb.org/video/space-...