David Gally
@strain9000.bsky.social
E. coli centric microbiologist now heavily into predicting phage efficacy for UTI in dogs & humans plus O157 in cattle using genomes, interaction datasets and machine learning. Have always loved how bacteria generate heterogeneity…..it’s the spice of life.
Reposted by David Gally
New review article in The Lancet Microbe
Safety and efficacy of #phage application in bacterial decolonisation: a systematic review
www.thelancet.com/journals/lan...
#bacteriophage #OpenAccess #OA
Safety and efficacy of #phage application in bacterial decolonisation: a systematic review
www.thelancet.com/journals/lan...
#bacteriophage #OpenAccess #OA
March 5, 2024 at 8:39 AM
New review article in The Lancet Microbe
Safety and efficacy of #phage application in bacterial decolonisation: a systematic review
www.thelancet.com/journals/lan...
#bacteriophage #OpenAccess #OA
Safety and efficacy of #phage application in bacterial decolonisation: a systematic review
www.thelancet.com/journals/lan...
#bacteriophage #OpenAccess #OA
Reposted by David Gally
Super interesting study on the use of phage in the environmental surveillance of Salmonella Typhi journals.plos.org/plosntds/art...
Detection of Salmonella Typhi bacteriophages in surface waters as a scalable approach to environmental surveillance
Author summary Typhoid fever is a major health issue in low- and middle-income countries, causing illness and death. The World Health Organization recommends the use of typhoid-conjugate vaccines to c...
journals.plos.org
March 6, 2024 at 9:15 AM
Super interesting study on the use of phage in the environmental surveillance of Salmonella Typhi journals.plos.org/plosntds/art...
Smart stuff, we have been looking at scavenging systems and signalling in E. coli with respect to phage sensitivity but this is nice & specific…
doi.org/10.1038/s414...
doi.org/10.1038/s414...
March 12, 2024 at 12:01 PM
Smart stuff, we have been looking at scavenging systems and signalling in E. coli with respect to phage sensitivity but this is nice & specific…
doi.org/10.1038/s414...
doi.org/10.1038/s414...
Reposted by David Gally
The links between Epstein-Barr virus infection and later multiple sclerosis are getting stronger:
www.science.org/content/blog...
www.science.org/content/blog...
January 8, 2024 at 7:33 PM
The links between Epstein-Barr virus infection and later multiple sclerosis are getting stronger:
www.science.org/content/blog...
www.science.org/content/blog...
January 2, 2024 at 9:39 AM
Question on plagiarism software. We use Turnitin at our university but Food Standards Scotland are interested in using something like this but Turnitin very expensive. Does anyone have experience with other software? What do the journals use?
December 18, 2023 at 4:27 PM
Question on plagiarism software. We use Turnitin at our university but Food Standards Scotland are interested in using something like this but Turnitin very expensive. Does anyone have experience with other software? What do the journals use?
Reposted by David Gally
Re: the UK government’s new visa rules, I’ve written about my own experience—spending more than £13,000 on visas and a ridiculous test that had incorrect answers—but also why I still love the UK, even as the government tried to deter me from living here. www.forkingpaths.co/p/my-life-in...
My "Life in the UK" Test and a Great British Travel Guide
I passed my "Life in the UK" test and will soon (hopefully) become a dual citizen of the US and UK. I reflect on 12 years in Britain and offer some less obvious UK travel suggestions for you to enjoy.
www.forkingpaths.co
December 13, 2023 at 9:37 AM
Re: the UK government’s new visa rules, I’ve written about my own experience—spending more than £13,000 on visas and a ridiculous test that had incorrect answers—but also why I still love the UK, even as the government tried to deter me from living here. www.forkingpaths.co/p/my-life-in...
Reposted by David Gally
An excellent summary as to why early career scientists should publish in Society Journals. Please read it. journals.asm.org/doi/10.1128/...
December 13, 2023 at 5:49 PM
An excellent summary as to why early career scientists should publish in Society Journals. Please read it. journals.asm.org/doi/10.1128/...
Reposted by David Gally
Thrilled to see our Phage CRISPRi paper in @PLOSbiology doi.org/10.1371/jour...
Systematic and scalable genome-wide essentiality mapping to identify nonessential genes in phages
In this work, authors use an arrayed genome-wide CRISPR interference assay to map gene essentiality landscape in the canonical coliphages λ and P1.
doi.org
December 4, 2023 at 11:38 PM
Thrilled to see our Phage CRISPRi paper in @PLOSbiology doi.org/10.1371/jour...
So following up on Aude Bernheim’s preprint yesterday, we uploaded our related work on predictive phage therapy for uropathogenic E. coli.
Our phage interactions for the predictive models are >9000 interactions in artificial urine…would be great to add more generalist phages…
Our phage interactions for the predictive models are >9000 interactions in artificial urine…would be great to add more generalist phages…
Predictive phage therapy for Escherichia coli urinary tract infections: cocktail selection for thera...
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
November 24, 2023 at 7:57 AM
So following up on Aude Bernheim’s preprint yesterday, we uploaded our related work on predictive phage therapy for uropathogenic E. coli.
Our phage interactions for the predictive models are >9000 interactions in artificial urine…would be great to add more generalist phages…
Our phage interactions for the predictive models are >9000 interactions in artificial urine…would be great to add more generalist phages…
A heritable iron memory enables decision-making in Escherichia coli
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
doi.org
November 21, 2023 at 11:12 PM
A heritable iron memory enables decision-making in Escherichia coli
Going back to methods to generate heterogeneity - I always thought this paper by JJ Collins was overlooked (even though in Nature) - you just need the right interacting regulatory systems to generate a biphasic population: rdcu.be/dqEOZ
Construction of a genetic toggle switch in Escherichia coli - Nature
Nature - Construction of a genetic toggle switch in Escherichia coli
doi.org
November 9, 2023 at 8:07 AM
Going back to methods to generate heterogeneity - I always thought this paper by JJ Collins was overlooked (even though in Nature) - you just need the right interacting regulatory systems to generate a biphasic population: rdcu.be/dqEOZ
Similar can be achieved by subsets that express colanic acid that limits many phage interactions. We are looking at the interplay between levels of O157 antigen, Gp4 capsule and colanic acid expression for phage resistance in vivo. Basis to heterogeneity still to be determined……
How would an E. coli flip from O antigen producing to non producing in a population…………..don’t think that’s ever been looked at as a heterogeneous characteristic
November 9, 2023 at 7:50 AM
Similar can be achieved by subsets that express colanic acid that limits many phage interactions. We are looking at the interplay between levels of O157 antigen, Gp4 capsule and colanic acid expression for phage resistance in vivo. Basis to heterogeneity still to be determined……