Nathan Fraikin
@nfrk92.bsky.social
Post-doc at TacC team / CNRS / Lyon 1- Catching bacteria on tape right in the act !
Reposted by Nathan Fraikin
🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Expanding the genetic toolbox of the obligate predatory bacterium Bdellovibrio bacteriovorus with inducible gene expression and CRISPR interference
Inducible gene expression and CRISPR interference-mediated depletion in the predatory bacterium Bdellovibrio bacteriovorus.
academic.oup.com
September 17, 2025 at 7:27 AM
🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Reposted by Nathan Fraikin
Exclusion systems, far from slowing down plasmid spread, are in fact essential to preserve host cell viability and thereby ensure the successful dissemination of conjugative plasmids and antibiotic resistance genes.
@nfrk92.bsky.social
@narjournal.bsky.social
academic.oup.com/nar/article/...
@nfrk92.bsky.social
@narjournal.bsky.social
academic.oup.com/nar/article/...
Exclusion systems preserve host cell homeostasis and fitness, ensuring successful dissemination of conjugative plasmids and associated resistance genes
Abstract. Plasmid conjugation is a major driver of antibiotic resistance dissemination in bacteria. In addition to genes required for transfer and maintena
academic.oup.com
September 17, 2025 at 10:17 AM
Exclusion systems, far from slowing down plasmid spread, are in fact essential to preserve host cell viability and thereby ensure the successful dissemination of conjugative plasmids and antibiotic resistance genes.
@nfrk92.bsky.social
@narjournal.bsky.social
academic.oup.com/nar/article/...
@nfrk92.bsky.social
@narjournal.bsky.social
academic.oup.com/nar/article/...
Reposted by Nathan Fraikin
Biofilm architecture determines the dissemination of conjugative plasmids: We measured the spatiotemporal spread of plasmids in biofilms and found that plasmids only spread in some biofilm regions!
Collab with lab of @sbigot.bsky.social & Christian Lesterlin
www.pnas.org/doi/10.1073/...
Collab with lab of @sbigot.bsky.social & Christian Lesterlin
www.pnas.org/doi/10.1073/...
Biofilm architecture determines the dissemination of conjugative plasmids | PNAS
Plasmid conjugation is a contact-dependent horizontal gene transfer mechanism that
significantly contributes to the dissemination of antibiotic res...
www.pnas.org
April 28, 2025 at 7:03 AM
Biofilm architecture determines the dissemination of conjugative plasmids: We measured the spatiotemporal spread of plasmids in biofilms and found that plasmids only spread in some biofilm regions!
Collab with lab of @sbigot.bsky.social & Christian Lesterlin
www.pnas.org/doi/10.1073/...
Collab with lab of @sbigot.bsky.social & Christian Lesterlin
www.pnas.org/doi/10.1073/...
Our set of new prokaryote-optimized fluorescent proteins is now published ! #microbiology #microsky #microscopy 🧫🦠🔬
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
A palette of bright and photostable monomeric fluorescent proteins for bacterial time-lapse imaging
Bright and photostable monomeric fluorescent proteins enable long-term time-lapse imaging in E. coli.
www.science.org
April 23, 2025 at 3:02 PM
Our set of new prokaryote-optimized fluorescent proteins is now published ! #microbiology #microsky #microscopy 🧫🦠🔬
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Nathan Fraikin
MMSB - Molecular Microbiology and Structural Biochemistry @mmsb-lyon.bsky.social is now on Bluesky!
👉 Follow us to know about our last publications, prizes, job offers, seminars, events,...
👉 Follow us to know about our last publications, prizes, job offers, seminars, events,...
April 23, 2025 at 2:28 PM
MMSB - Molecular Microbiology and Structural Biochemistry @mmsb-lyon.bsky.social is now on Bluesky!
👉 Follow us to know about our last publications, prizes, job offers, seminars, events,...
👉 Follow us to know about our last publications, prizes, job offers, seminars, events,...
Reposted by Nathan Fraikin
New preprint from the lab!! Check it out!
🚨 New preprint! We know that plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by @jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Dissecting pOXA-48 fitness effects in clinical enterobacteria using plasmid-wide CRISPRi screens
Conjugative plasmids are the main vehicle for the spread of antimicrobial resistance (AMR) genes in clinical bacteria. AMR plasmids allow bacteria to survive antibiotic treatments, but they also produ...
www.biorxiv.org
January 24, 2025 at 12:49 PM
New preprint from the lab!! Check it out!
Reposted by Nathan Fraikin
New preprint from our Lesterlin Lab! Yannick Baffert and @nfrk92.bsky.social, in collaboration with @macerwan.bsky.social and @val-meve.bsky.social, shed light on the molecular mechanisms driving pOXA-48 plasmid maintenance and transfer. We reveal novel TA system, regulators, and T4SS components!
Genetic Determinants of pOXA-48 Plasmid Maintenance and Propagation in Escherichia coli
Conjugative plasmids are the main drivers of antibiotic resistance dissemination contributing to the emergence and extensive spread of multidrug resistance clinical bacterial pathogens. pOXA-48 plasmi...
www.biorxiv.org
January 25, 2025 at 9:20 AM
New preprint from our Lesterlin Lab! Yannick Baffert and @nfrk92.bsky.social, in collaboration with @macerwan.bsky.social and @val-meve.bsky.social, shed light on the molecular mechanisms driving pOXA-48 plasmid maintenance and transfer. We reveal novel TA system, regulators, and T4SS components!
Reposted by Nathan Fraikin
RIP Pierre. You were one of the best. Thank you for all you did for our field but more importantly, for me and all the people you helped. I will miss you. ❤️
December 8, 2023 at 12:09 AM
RIP Pierre. You were one of the best. Thank you for all you did for our field but more importantly, for me and all the people you helped. I will miss you. ❤️