Mikhail Kolokolov
@mikolok.bsky.social
PhD student at International Tomography Center SB RAS
Interested in exploring complicated structural biology problems using EPR and other methods
Interested in exploring complicated structural biology problems using EPR and other methods
Reposted by Mikhail Kolokolov
Researchers from ETH Zurich have succeeded in introducing large quantities of unnatural #AminoAcids into #Bacteria, enabling the creation of innovative and highly efficient designer proteins. These can be used as more efficient catalysts or more effective #Drugs.
A Trojan horse for artificial amino acids
Researchers from ETH Zurich have succeeded in introducing large quantities of unnatural amino acids into bacteria, enabling the creation of innovative and highly efficient designer proteins. These can...
ethz.ch
December 10, 2025 at 10:19 AM
Researchers from ETH Zurich have succeeded in introducing large quantities of unnatural #AminoAcids into #Bacteria, enabling the creation of innovative and highly efficient designer proteins. These can be used as more efficient catalysts or more effective #Drugs.
Also got a chance to present our recent #EPR research at the 'Future of Biomedicine' conference ๐ค. It was a fantastic gathering of Russia's top biological community at the impressive FEFU campus on Russky Island. It even has waterfalls! ๐
September 27, 2025 at 10:30 AM
Also got a chance to present our recent #EPR research at the 'Future of Biomedicine' conference ๐ค. It was a fantastic gathering of Russia's top biological community at the impressive FEFU campus on Russky Island. It even has waterfalls! ๐
Had a great trip to Vladivostok, one of the eastern cities of Russia.
Open sea, magnificent views, very great weather... which results in too, too many photos ๐ซ
Open sea, magnificent views, very great weather... which results in too, too many photos ๐ซ
September 27, 2025 at 10:20 AM
Had a great trip to Vladivostok, one of the eastern cities of Russia.
Open sea, magnificent views, very great weather... which results in too, too many photos ๐ซ
Open sea, magnificent views, very great weather... which results in too, too many photos ๐ซ
Thank you for the honor!
&:
Mikhail Kolokolov from the International Tomography Center, Novosibirsk, Russia
Enhanced Binding Site Identification in ProteinโLigand Complexes with a Combined Blind Docking and Dipolar Electron Paramagnetic Resonance Approach,ย Journal of the American Chemical Society 2025 147 (16),13677-13687
Mikhail Kolokolov from the International Tomography Center, Novosibirsk, Russia
Enhanced Binding Site Identification in ProteinโLigand Complexes with a Combined Blind Docking and Dipolar Electron Paramagnetic Resonance Approach,ย Journal of the American Chemical Society 2025 147 (16),13677-13687
August 1, 2025 at 12:58 PM
Thank you for the honor!
Reposted by Mikhail Kolokolov
Winners of Best Paper awards:
ieprs.org/best-paper-awaโฆ [ieprs.org]
Daniele Panariti from the University of Oxford, UK
Control of excitation selectivity in pulse EPR on spin-correlated radical pairs with shaped pulses, Phys. Chem. Chem. Phys. (2024) 26, 3842-3856
ieprs.org/best-paper-awaโฆ [ieprs.org]
Daniele Panariti from the University of Oxford, UK
Control of excitation selectivity in pulse EPR on spin-correlated radical pairs with shaped pulses, Phys. Chem. Chem. Phys. (2024) 26, 3842-3856
https://ieprs.org/best-paper-awaโฆ
July 18, 2025 at 1:49 PM
Winners of Best Paper awards:
ieprs.org/best-paper-awaโฆ [ieprs.org]
Daniele Panariti from the University of Oxford, UK
Control of excitation selectivity in pulse EPR on spin-correlated radical pairs with shaped pulses, Phys. Chem. Chem. Phys. (2024) 26, 3842-3856
ieprs.org/best-paper-awaโฆ [ieprs.org]
Daniele Panariti from the University of Oxford, UK
Control of excitation selectivity in pulse EPR on spin-correlated radical pairs with shaped pulses, Phys. Chem. Chem. Phys. (2024) 26, 3842-3856
Reposted by Mikhail Kolokolov
In our study @elife.bsky.social we uncover the mechanism of mPPase inhibition. Over 20x structures to date are in a symmetric form. We use EPR(PELDOR/DEER) and reveal asymmetric binding at the active site while maintaining symmetric conformation at periplasmic site
elifesciences.org/reviewed-pre...
elifesciences.org/reviewed-pre...
July 15, 2025 at 1:44 PM
In our study @elife.bsky.social we uncover the mechanism of mPPase inhibition. Over 20x structures to date are in a symmetric form. We use EPR(PELDOR/DEER) and reveal asymmetric binding at the active site while maintaining symmetric conformation at periplasmic site
elifesciences.org/reviewed-pre...
elifesciences.org/reviewed-pre...
Our institute is located in Novosibirsk, which, as you might guess, is quite cold! In here, snow in May is quite a common occurrence
May 13, 2025 at 3:45 PM
Our institute is located in Novosibirsk, which, as you might guess, is quite cold! In here, snow in May is quite a common occurrence
We also use EPR to investigate supramolecular biological objects, such as ribosome-RNA complexes. In our recent work we have compared different spin labeling approaches for the investigation of ribosome-mRNA binding. Turns out, contradictory results may, in fact, be okay!
doi.org/10.1063/5.02...
doi.org/10.1063/5.02...
Exploring combined spin-labeling approach for structural studies of mRNA in the human ribosome
In this study, we investigated the structural variability of mRNA in the human ribosome by comparing two spin-labeling strategies: one involving an mRNA analog
doi.org
May 13, 2025 at 3:40 PM
We also use EPR to investigate supramolecular biological objects, such as ribosome-RNA complexes. In our recent work we have compared different spin labeling approaches for the investigation of ribosome-mRNA binding. Turns out, contradictory results may, in fact, be okay!
doi.org/10.1063/5.02...
doi.org/10.1063/5.02...
In our most recent work we have developed new approach to the detection of binding sites using information from #EPR as a filtering and validation tool combined with molecular modeling algorithms
doi.org/10.1021/jacs...
doi.org/10.1021/jacs...
Enhanced Binding Site Identification in ProteinโLigand Complexes with a Combined Blind Docking and Dipolar Electron Paramagnetic Resonance Approach
Understanding proteinโdrug complex structures is crucial for elucidating therapeutic mechanisms and side effects. Blind docking facilitates site identification but is hindered by computational complex...
doi.org
May 13, 2025 at 3:40 PM
In our most recent work we have developed new approach to the detection of binding sites using information from #EPR as a filtering and validation tool combined with molecular modeling algorithms
doi.org/10.1021/jacs...
doi.org/10.1021/jacs...
One of our focus points is an application of laser EPR spectroscopy for the investigation of photoactive biological systems. For example, we have shown that EPR allows detection of laser-induced changes in (strange) DNA quadruplexes complexed with photosensitizers doi.org/10.1039/D3CP...
Revealing light-induced structural shifts in G-quadruplex-porphyrin complexes: a pulsed dipolar EPR study
The binding of G-quadruplex structures (G4s) with photosensitizers is of considerable importance in medicinal chemistry and drug discovery due to their promising potential in photodynamic therapy appl...
doi.org
May 13, 2025 at 3:40 PM
One of our focus points is an application of laser EPR spectroscopy for the investigation of photoactive biological systems. For example, we have shown that EPR allows detection of laser-induced changes in (strange) DNA quadruplexes complexed with photosensitizers doi.org/10.1039/D3CP...