New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz

A great use of various heterogeneity methods to uncover a rare binding partner at high resolution! Check it out!

On a gruelling hunt for rare associated molecules in your #cryoEM particle stack and not sure where to look?

Discover practical tips and tricks in our new case study using #CryoSPARC v4.7.1 where we find and refine a low-population interaction partner!

guide.cryosparc.com/processing-d...

So excited to see this project finally come together after 2+ years of development! I’m proud of my contributions, but this success belongs to the entire Structura team — such a great example of what collaboration and creativity can achieve.

Absolutely brilliant work! Nice job @olibclarke.bsky.social, @fravallese.bsky.social , and others!

Our #CryoET story on how a top antimalarial drug candidate perturbs the native malarial translation machinery is out @natsmb.nature.com🥳 rdcu.be/eBbrH

A massive team effort led by @leonieanton.bsky.social Meseret Haile & Wenjing Cheng in collaboration with Jerzy Dziekan & the Alan Cowman! #TeamTomo

When particles have orientation bias, the resulting maps are often streaked along the missing directions. In this case study, we cover why orientation bias produces anisotropic #cryoEM maps and how you may be able to recover from it!

guide.cryosparc.com/processing-d...

Grappling with poorly-resolved ligand binding sites in #cryoEM maps?
Check out our new case study on a peptide ligand-bound GPCR showing how practical tips and features in #CryoSPARC v4.7 improve locally refined map quality and reveal clearer ligand density!
guide.cryosparc.com/processing-d...

Are you new to #CryoEM, or looking for a review of the basics?

You might enjoy our TRPV1 case study! It covers preprocessing, particle curation, refinement, and more! You can also test your skills with the advanced exercises at the end of the study.
guide.cryosparc.com/processing-d...

#CryoSPARC

#CryoSPARC v4.7 is out! 🚀 
Featuring a new automatic Micrograph Junk Detector that labels and rejects contaminants, new tools for curating subsets of particles, performance and stability fixes, and more!
Full changelog: cryosparc.com/updates

#cryoEM

1/ A new series of #CryoSPARC tutorial videos from this year’s S2C2 #cryoEM image processing workshop are now online! These videos will be interesting to all users and especially those newer to #cryoEM.

www.youtube.com/playlist?lis...

Looking for a primer on why the contrast transfer function matters in #cryoEM?

Have a look at our updated #CryoSPARC Guide pages on CTF estimation! We cover intuition, theory, job types and practical considerations: guide.cryosparc.com/processing-d...

4 yrs ago today, a family member managed a truly spectacular own goal, splintered his domestic bliss, & in the process, united the world for a day.

On popular request, I shall now recreate the livetweet thread from that day

So gather around children, for this is the tale of The Rice Truck Saga