Communications Chemistry, Published online: 12 April 2025; doi:10.1038/s42004-025-01507-0RNA modifications play critical roles in gene expression regulation, RNA stability, and translation efficiency, however, the detection of RNA modifications by various sequencing approaches may present differing results. Here, the authors explore several computational methods for the detection of the most researched mRNA modifications, with a view to improving algorithms’ comparability, reliability, and consistency in RNA modification prediction.

RNA modifications play critical roles in gene expression regulation, RNA stability, and translation efficiency, however, the detection of RNA modifications by various sequencing approaches may present...

"Trained immunity" of innate immune cells occurs through a sequential two-step process where an initial pathogenic or sterile inflammatory trigger is followed by an amplified response to a later un-re...

The impact of synonymous mutations remains elusive. Here, the authors demonstrate that synonymous mutations can promote tumorigenesis by disrupting post-transcriptional m6A modification. The findings ...

Single cell and spatial transcriptomics have dramatically changed how we can profile RNA from heterogenous biological samples. Combining single cell and spatial profiling with long read RNA-Seq promis...

YTHDF2 modulates oncogenic transformation and immune evasion across multiple B cell cancers by stabilizing m5C-modified mRNAs and destabilizing m6A-modified mRNAs, thereby serving as a dual reader for...

Our genes contain all the instructions our body needs to function, but their expression must be finely regulated to guarantee that each cell performs its role optimally. This is where DNA and RNA epig...

Community-curated list of software packages and data resources for single-cell, including RNA-seq, ATAC-seq, etc. - seandavi/awesome-single-cell

RNA polyadenylation is crucial for RNA maturation, stability and function, with polyA tail lengths significantly influencing mRNA translation, efficiency and decay. Here, we provide a step-by-step pro...