Jaroslav Icha
@ichajaroslav.bsky.social
Light microscopy at Bruker Fluorescence Microscopy division.
#lightsheet #multiphoton #superresolution #screening
Cell biologist in my previous life. Alumnus @mpicbg.bsky.social
@turkubioscience.bsky.social
#lightsheet #multiphoton #superresolution #screening
Cell biologist in my previous life. Alumnus @mpicbg.bsky.social
@turkubioscience.bsky.social
Pinned
Jaroslav Icha
@ichajaroslav.bsky.social
· Aug 16
We have just installed the high throughput microscope Acquifer IM from Bruker in the lab of Prof. Buchtova in Brno, Inst. of Animal Physiology and Genetics. This microscope is a versatile widefield fluorescence microscope, which is suited for imaging larger samples like organoids or fish embryos.
Are you interested in long time lapse imaging of organoids?
In this example, retinal organoids were imaged in high throughput for 3 days. The aim was to collect sufficient data to be able to use machine learning for predicting organoid differentiation just from brightfield images.
In this example, retinal organoids were imaged in high throughput for 3 days. The aim was to collect sufficient data to be able to use machine learning for predicting organoid differentiation just from brightfield images.
October 15, 2025 at 1:14 PM
Are you interested in long time lapse imaging of organoids?
In this example, retinal organoids were imaged in high throughput for 3 days. The aim was to collect sufficient data to be able to use machine learning for predicting organoid differentiation just from brightfield images.
In this example, retinal organoids were imaged in high throughput for 3 days. The aim was to collect sufficient data to be able to use machine learning for predicting organoid differentiation just from brightfield images.
Reposted by Jaroslav Icha
🔥 It's Time ! #Mifobio2025
👉 imabio-cnrs.fr
#GDRImabio
@cnrs.fr
@cnrsingenierie.bsky.social
@cnrsbiologie.bsky.social
👉 imabio-cnrs.fr
#GDRImabio
@cnrs.fr
@cnrsingenierie.bsky.social
@cnrsbiologie.bsky.social
October 10, 2025 at 2:05 AM
For those at Seeing is Believing in Heidelberg right now, do join our workshop, which is about to start soon at 11:30!
www.embl.org/about/info/c...
Focus on organoid time lapse imaging, with high throughput options as well.
#EESImaging
www.embl.org/about/info/c...
Focus on organoid time lapse imaging, with high throughput options as well.
#EESImaging
www.embl.org
October 8, 2025 at 8:18 AM
For those at Seeing is Believing in Heidelberg right now, do join our workshop, which is about to start soon at 11:30!
www.embl.org/about/info/c...
Focus on organoid time lapse imaging, with high throughput options as well.
#EESImaging
www.embl.org/about/info/c...
Focus on organoid time lapse imaging, with high throughput options as well.
#EESImaging
Reposted by Jaroslav Icha
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
September 22, 2025 at 6:27 PM
🚀 Our new paper is out @natmethods.nature.com!
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.
🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/
Reposted by Jaroslav Icha
✨🔬 #FluorescenceFriday
Confocal stack of chicken retina 🐥 — from inner nerve fibers up to photoreceptors.
Layer upon layer of mosaics, each cell type in its place.
Birds have remarkable visual systems, and under the microscope, they look absolutely stunning in detail.
Confocal stack of chicken retina 🐥 — from inner nerve fibers up to photoreceptors.
Layer upon layer of mosaics, each cell type in its place.
Birds have remarkable visual systems, and under the microscope, they look absolutely stunning in detail.
September 19, 2025 at 5:36 PM
✨🔬 #FluorescenceFriday
Confocal stack of chicken retina 🐥 — from inner nerve fibers up to photoreceptors.
Layer upon layer of mosaics, each cell type in its place.
Birds have remarkable visual systems, and under the microscope, they look absolutely stunning in detail.
Confocal stack of chicken retina 🐥 — from inner nerve fibers up to photoreceptors.
Layer upon layer of mosaics, each cell type in its place.
Birds have remarkable visual systems, and under the microscope, they look absolutely stunning in detail.
Reposted by Jaroslav Icha
The #SCZI2026 Oral Abstract and Workshop proposal deadline has been extended! They are now due September 24th at 11:59pm EDT. Register and Submit here: www.izfs.org/education/11...
11th Strategic Conference of Zebrafish Investigators | IZFS - International Zebrafish Society
www.izfs.org
September 18, 2025 at 2:04 PM
The #SCZI2026 Oral Abstract and Workshop proposal deadline has been extended! They are now due September 24th at 11:59pm EDT. Register and Submit here: www.izfs.org/education/11...
We have just installed a third HIVE server in Czechia. After Prague and Brno, we are adding Olomouc!
www.bruker.com/de/products-...
Researchers often get HIVE to handle data from Luxendo as well as other companies' light-sheet scopes but it is a rather universal solution for big data management.
www.bruker.com/de/products-...
Researchers often get HIVE to handle data from Luxendo as well as other companies' light-sheet scopes but it is a rather universal solution for big data management.
September 11, 2025 at 9:54 AM
We have just installed a third HIVE server in Czechia. After Prague and Brno, we are adding Olomouc!
www.bruker.com/de/products-...
Researchers often get HIVE to handle data from Luxendo as well as other companies' light-sheet scopes but it is a rather universal solution for big data management.
www.bruker.com/de/products-...
Researchers often get HIVE to handle data from Luxendo as well as other companies' light-sheet scopes but it is a rather universal solution for big data management.
Reposted by Jaroslav Icha
We are all super happy and proud to see our work on the function and evolution of the #cephalic #furrow published in @nature.com. Let me say a few things about the background and history of this work on the #Evolution_of_Morphogenesis (1/12)
September 4, 2025 at 8:22 AM
We are all super happy and proud to see our work on the function and evolution of the #cephalic #furrow published in @nature.com. Let me say a few things about the background and history of this work on the #Evolution_of_Morphogenesis (1/12)
Reposted by Jaroslav Icha
🚨 Final bits are falling into place for #UKZF2025 🎉 The organisers are buzzing with excitement and can’t wait to welcome the zebrafish community to Exeter & Exmouth TODAY 🐟✨
September 10, 2025 at 8:58 AM
🚨 Final bits are falling into place for #UKZF2025 🎉 The organisers are buzzing with excitement and can’t wait to welcome the zebrafish community to Exeter & Exmouth TODAY 🐟✨
Reposted by Jaroslav Icha
Wow, that was amazing! We thank everyone for attending the V4SDB 2025 meeting! You all made it an excellent conference, where we had the opportunity to hear from outstanding speakers,share ideas and experiences, and of course have some unforgettable time during social activities!
September 7, 2025 at 3:12 PM
Wow, that was amazing! We thank everyone for attending the V4SDB 2025 meeting! You all made it an excellent conference, where we had the opportunity to hear from outstanding speakers,share ideas and experiences, and of course have some unforgettable time during social activities!
Happy to be at the #V4SDB 2025 developmental biology conference. Amazing quality of the talks and a beautiful venue in the High Tatras in Slovakia.
@v4sdb.bsky.social
v4sdb2025.img.cas.cz/programme/
@v4sdb.bsky.social
v4sdb2025.img.cas.cz/programme/
September 6, 2025 at 7:56 AM
Happy to be at the #V4SDB 2025 developmental biology conference. Amazing quality of the talks and a beautiful venue in the High Tatras in Slovakia.
@v4sdb.bsky.social
v4sdb2025.img.cas.cz/programme/
@v4sdb.bsky.social
v4sdb2025.img.cas.cz/programme/
Reposted by Jaroslav Icha
Good morning everyone! Welcome to Day 2 of the V4SDB Meeting 2025! Yesterday was full of events, but grab some coffee - there's still so much more to discover!
#V4SDB2025 #conference #developmentalbiology #evodevo #morphogenesis #organoids
#V4SDB2025 #conference #developmentalbiology #evodevo #morphogenesis #organoids
September 6, 2025 at 6:55 AM
Good morning everyone! Welcome to Day 2 of the V4SDB Meeting 2025! Yesterday was full of events, but grab some coffee - there's still so much more to discover!
#V4SDB2025 #conference #developmentalbiology #evodevo #morphogenesis #organoids
#V4SDB2025 #conference #developmentalbiology #evodevo #morphogenesis #organoids
Reposted by Jaroslav Icha
Dresden researchers @paveltomancak.bsky.social @bruvellu.bsky.social, Carl Modes, @cuencam15.bsky.social & colleagues published in @nature.com that a tissue fold in fruit fly embryos buffers mechanical stresses & may have evolved in response to mechanical forces. www.mpi-cbg.de/news-outreac...
Mechanical forces drive evolutionary change
A small tissue fold present in fruit fly embryos buffers mechanical stresses and may have evolved in response to mechanical forces.
www.mpi-cbg.de
September 3, 2025 at 3:26 PM
Dresden researchers @paveltomancak.bsky.social @bruvellu.bsky.social, Carl Modes, @cuencam15.bsky.social & colleagues published in @nature.com that a tissue fold in fruit fly embryos buffers mechanical stresses & may have evolved in response to mechanical forces. www.mpi-cbg.de/news-outreac...
Reposted by Jaroslav Icha
The Company of Biologists Workshops 2027: Excited to share that our workshop concept, "Bridging Scales and Disciplines to Understand Robust Development”, has been selected! 🤩@giuliapaci.bsky.social and I are looking forward to organizing it. Stay tuned for details! @biologists.bsky.social
August 22, 2025 at 1:04 PM
The Company of Biologists Workshops 2027: Excited to share that our workshop concept, "Bridging Scales and Disciplines to Understand Robust Development”, has been selected! 🤩@giuliapaci.bsky.social and I are looking forward to organizing it. Stay tuned for details! @biologists.bsky.social
Reposted by Jaroslav Icha
#LISH25 final student team presentations. #The_Holy_de_Broglies
August 22, 2025 at 1:11 PM
#LISH25 final student team presentations. #The_Holy_de_Broglies
Reposted by Jaroslav Icha
#LISH25 day 10 ushered in by Claudia Gerri from @mpi-cbg.de introducing us to the wonderful world of mammalian early embryo development, implantation and #placenta interactions. Showing light sheet data from ex #LoPaT - Michi Burkon 💕. Walk-up song with Italian flair came with sweets.
August 20, 2025 at 7:19 AM
#LISH25 day 10 ushered in by Claudia Gerri from @mpi-cbg.de introducing us to the wonderful world of mammalian early embryo development, implantation and #placenta interactions. Showing light sheet data from ex #LoPaT - Michi Burkon 💕. Walk-up song with Italian flair came with sweets.
Really nice thread about the infrared co-illumination to reduce photobleaching of GFP.
I was aware of the Ludvíkova et al paper but didn’t realize that lightsheet is the main modality, which would benefit from it.
I was aware of the Ludvíkova et al paper but didn’t realize that lightsheet is the main modality, which would benefit from it.
How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!
August 19, 2025 at 9:00 PM
Really nice thread about the infrared co-illumination to reduce photobleaching of GFP.
I was aware of the Ludvíkova et al paper but didn’t realize that lightsheet is the main modality, which would benefit from it.
I was aware of the Ludvíkova et al paper but didn’t realize that lightsheet is the main modality, which would benefit from it.
Reposted by Jaroslav Icha
How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!
August 19, 2025 at 7:31 PM
How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!
Reposted by Jaroslav Icha
Excellent mid-course presentations from the #LISH25 student teams. Packed @csbdresden.bsky.social seminar room. Tons of data, visualisation, sample preparation creativity, and image analysis in progress. Lots of ambition from the student teams. I am impressed.
August 18, 2025 at 8:55 PM
Excellent mid-course presentations from the #LISH25 student teams. Packed @csbdresden.bsky.social seminar room. Tons of data, visualisation, sample preparation creativity, and image analysis in progress. Lots of ambition from the student teams. I am impressed.
Reposted by Jaroslav Icha
Would your #cellbio research benefit from you attending a #Microscopy or #BioimageAnalysis training course? For grants of up to £1,000, check out our Microscopy Training Grants with @focalplane.bsky.social
Next deadline: 5 September 2025
www.biologists.com/grants/jcs-f...
Next deadline: 5 September 2025
www.biologists.com/grants/jcs-f...
August 14, 2025 at 11:25 AM
Would your #cellbio research benefit from you attending a #Microscopy or #BioimageAnalysis training course? For grants of up to £1,000, check out our Microscopy Training Grants with @focalplane.bsky.social
Next deadline: 5 September 2025
www.biologists.com/grants/jcs-f...
Next deadline: 5 September 2025
www.biologists.com/grants/jcs-f...
Reposted by Jaroslav Icha
Reposted by Jaroslav Icha
Diagnosis of the beep at #LISH25: it wasn't the #HIVEs as such (still room for more users & data!) but the heavy work for the GPU in a warm room: #napari #imaris #CARE #ZEN #slidebook and #MACSiQ software (20 users) on a single GPU making this GPU sweat 😅 💪 We'll now share the load with more GPUs
August 18, 2025 at 6:21 AM
Reposted by Jaroslav Icha
It’s Sunday, but #LISH25 goes on with Matteo Rauzi presentation on using light sheet to study #Morphogenesis of #flies and #sea_urchins. Matteo had not one but two walk-up songs! #RockyBalboa 🥊
August 17, 2025 at 9:13 AM
It’s Sunday, but #LISH25 goes on with Matteo Rauzi presentation on using light sheet to study #Morphogenesis of #flies and #sea_urchins. Matteo had not one but two walk-up songs! #RockyBalboa 🥊
We have just installed the high throughput microscope Acquifer IM from Bruker in the lab of Prof. Buchtova in Brno, Inst. of Animal Physiology and Genetics. This microscope is a versatile widefield fluorescence microscope, which is suited for imaging larger samples like organoids or fish embryos.
August 16, 2025 at 11:47 AM
We have just installed the high throughput microscope Acquifer IM from Bruker in the lab of Prof. Buchtova in Brno, Inst. of Animal Physiology and Genetics. This microscope is a versatile widefield fluorescence microscope, which is suited for imaging larger samples like organoids or fish embryos.