Chris Langsdorf
@fluorist.bsky.social
I develop fluorescence based dyes and labeling reagents to visualize the complex lives of cells.
Had a couple of good questions tonight:
Dad, what would happen if you threw a gallon of water on the sun?
What would happen if a planet that was 700 degrees hot crashed into a planet that was 700 degrees below zero?
Dad, what would happen if you threw a gallon of water on the sun?
What would happen if a planet that was 700 degrees hot crashed into a planet that was 700 degrees below zero?
June 6, 2025 at 4:07 AM
Had a couple of good questions tonight:
Dad, what would happen if you threw a gallon of water on the sun?
What would happen if a planet that was 700 degrees hot crashed into a planet that was 700 degrees below zero?
Dad, what would happen if you threw a gallon of water on the sun?
What would happen if a planet that was 700 degrees hot crashed into a planet that was 700 degrees below zero?
Brief, sunny NYC trip, now to Boston for a few days
May 14, 2025 at 12:29 AM
Brief, sunny NYC trip, now to Boston for a few days
Clever “cell floating assay” using CellTrace Violet to sort binders: De novo design of miniprotein agonists and antagonists targeting G protein-coupled receptors
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
April 13, 2025 at 3:29 PM
Clever “cell floating assay” using CellTrace Violet to sort binders: De novo design of miniprotein agonists and antagonists targeting G protein-coupled receptors
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Good afternoon for a jog past the @uoknightcampus.bsky.social
April 7, 2025 at 10:39 PM
Good afternoon for a jog past the @uoknightcampus.bsky.social
T shirt and sunglasses day on the mountain
March 25, 2025 at 11:40 PM
T shirt and sunglasses day on the mountain
Spring skiing on Mt Hood
March 24, 2025 at 9:41 PM
Spring skiing on Mt Hood
🌷Spring flowers? Fireworks?
Mouse cerebral cortex FFPE tissue was labeled with eight primary antibodies. Aluora Spatial Amplification Kits and DAPI were used for detection, followed by imaging on the EVOS S1000 Spatial Imaging System.
🧠🔬🧪 #Neuroscience #FluorescenceFriday
Mouse cerebral cortex FFPE tissue was labeled with eight primary antibodies. Aluora Spatial Amplification Kits and DAPI were used for detection, followed by imaging on the EVOS S1000 Spatial Imaging System.
🧠🔬🧪 #Neuroscience #FluorescenceFriday
March 21, 2025 at 5:27 AM
🌷Spring flowers? Fireworks?
Mouse cerebral cortex FFPE tissue was labeled with eight primary antibodies. Aluora Spatial Amplification Kits and DAPI were used for detection, followed by imaging on the EVOS S1000 Spatial Imaging System.
🧠🔬🧪 #Neuroscience #FluorescenceFriday
Mouse cerebral cortex FFPE tissue was labeled with eight primary antibodies. Aluora Spatial Amplification Kits and DAPI were used for detection, followed by imaging on the EVOS S1000 Spatial Imaging System.
🧠🔬🧪 #Neuroscience #FluorescenceFriday
[Event] Pushing the frontiers of bioimaging 2025
@pasteur.fr Institute organizes a symposium on new advances in life sciences and light microscopy! It is followed by demos by #microscopy companies on their latest tech🔬
📅 When? March 24-28
📍 Where? Institut Pasteur, Paris
🔗 How? bit.ly/4bJDjcS
@pasteur.fr Institute organizes a symposium on new advances in life sciences and light microscopy! It is followed by demos by #microscopy companies on their latest tech🔬
📅 When? March 24-28
📍 Where? Institut Pasteur, Paris
🔗 How? bit.ly/4bJDjcS
March 19, 2025 at 1:32 PM
Some microscopy art for St. Patrick's Day. This is antibody purification resin after cleaning up an Alexa Fluor 488 antibody conjugate. 🧪
March 17, 2025 at 8:51 PM
Some microscopy art for St. Patrick's Day. This is antibody purification resin after cleaning up an Alexa Fluor 488 antibody conjugate. 🧪
Three feet of powder means fun in the trees ❄️
March 16, 2025 at 8:25 PM
Three feet of powder means fun in the trees ❄️
For #FluorescenceFriday: a very thin slice of rat kidney was labeled with 8 primary antibodies whose signal was amplified with Aluora Spatial Amplification reagents. 🔬 on the EVOS S1000 Spatial Imaging system. >400 merged fields of view at 20x.
March 15, 2025 at 4:40 AM
For #FluorescenceFriday: a very thin slice of rat kidney was labeled with 8 primary antibodies whose signal was amplified with Aluora Spatial Amplification reagents. 🔬 on the EVOS S1000 Spatial Imaging system. >400 merged fields of view at 20x.
Reposted by Chris Langsdorf
🧬A new imaging technique developed by the Liu Lab uses a novel DNA barcode system to track hundreds of RNA & protein molecules in single cells within thick biological samples, providing a full picture of how these structures are organized inside tissues.
🔗 www.janelia.org/news/new-met...
🔗 www.janelia.org/news/new-met...
March 11, 2025 at 2:15 PM
🧬A new imaging technique developed by the Liu Lab uses a novel DNA barcode system to track hundreds of RNA & protein molecules in single cells within thick biological samples, providing a full picture of how these structures are organized inside tissues.
🔗 www.janelia.org/news/new-met...
🔗 www.janelia.org/news/new-met...
Mountain biking and skiing in the same weekend 🤘
March 10, 2025 at 2:53 AM
Mountain biking and skiing in the same weekend 🤘
Some hummingbirds appear colorful due to iridescence caused by the microscopic structure of their feathers. Here’s an Anna’s hummingbird feather from my yard at 460x 🧪
March 4, 2025 at 1:22 AM
Some hummingbirds appear colorful due to iridescence caused by the microscopic structure of their feathers. Here’s an Anna’s hummingbird feather from my yard at 460x 🧪
Under normal light fluorophore colors appear inverted: red dyes look blue, blue appear red, and green dyes are purple. Using an inverse LUT shows us this same palette on our microscopy sample. 🧪
March 1, 2025 at 9:09 PM
Under normal light fluorophore colors appear inverted: red dyes look blue, blue appear red, and green dyes are purple. Using an inverse LUT shows us this same palette on our microscopy sample. 🧪
Here's a set of three for #FluorescenceFriday. Same image, progressively higher zoom. FFPE human tonsil tissue labeled with primary antibodies against Cytokeratin, PCNA, C20, CD45RB, CD68, eCadherin, and SMA. 🧪🔬
March 1, 2025 at 5:31 AM
Here's a set of three for #FluorescenceFriday. Same image, progressively higher zoom. FFPE human tonsil tissue labeled with primary antibodies against Cytokeratin, PCNA, C20, CD45RB, CD68, eCadherin, and SMA. 🧪🔬
Today in @nature.com: remarkable extended survival in pancreatic cancer patients treated with cancer vaccine.
Autogene cevumeran induces CD8+ T cells with est. lifespan of 7.7 years (range 1.5 to roughly 100 years), with 20% of clones that may outlive hosts.
www.nature.com/articles/s41...
Autogene cevumeran induces CD8+ T cells with est. lifespan of 7.7 years (range 1.5 to roughly 100 years), with 20% of clones that may outlive hosts.
www.nature.com/articles/s41...
February 28, 2025 at 4:35 AM
Today in @nature.com: remarkable extended survival in pancreatic cancer patients treated with cancer vaccine.
Autogene cevumeran induces CD8+ T cells with est. lifespan of 7.7 years (range 1.5 to roughly 100 years), with 20% of clones that may outlive hosts.
www.nature.com/articles/s41...
Autogene cevumeran induces CD8+ T cells with est. lifespan of 7.7 years (range 1.5 to roughly 100 years), with 20% of clones that may outlive hosts.
www.nature.com/articles/s41...
The wetlands outside my office window often has nutria and geese. Today it was a pair of foxes. 🦊
February 25, 2025 at 6:45 PM
The wetlands outside my office window often has nutria and geese. Today it was a pair of foxes. 🦊
Reposted by Chris Langsdorf
FDA approves TROP2-targeted antibody–drug conjugate for breast cancer www.nature.com/articles/d41...
Daiichi Sankyo and AstraZeneca’s new ADC datopotamab deruxtecan will compete with Gilead/Immunomedics’ sacituzumab govitecan, which is expected to have sales of ~$1.7 billion in 2025
Daiichi Sankyo and AstraZeneca’s new ADC datopotamab deruxtecan will compete with Gilead/Immunomedics’ sacituzumab govitecan, which is expected to have sales of ~$1.7 billion in 2025
February 13, 2025 at 2:57 PM
FDA approves TROP2-targeted antibody–drug conjugate for breast cancer www.nature.com/articles/d41...
Daiichi Sankyo and AstraZeneca’s new ADC datopotamab deruxtecan will compete with Gilead/Immunomedics’ sacituzumab govitecan, which is expected to have sales of ~$1.7 billion in 2025
Daiichi Sankyo and AstraZeneca’s new ADC datopotamab deruxtecan will compete with Gilead/Immunomedics’ sacituzumab govitecan, which is expected to have sales of ~$1.7 billion in 2025
🦠🔬 Recently we reported the development of a new class of sensors to directly monitor live-cell protein trafficking and provide a sensitive indication of delivery to functional, enzymatically active lysosomes. A quick 🧵for #FluorescenceFriday www.biorxiv.org/content/10.1...
Monitoring lysosomal catabolism: a sensitive probe for assessing targeted lysosomal degradation of extracellular proteins
Extracellular targeted protein degradation (eTPD) is an emerging therapeutic field. The Lysosome targeting chimera (LYTAC) is a therapeutic modality that promotes degradation of extracellular drivers ...
www.biorxiv.org
February 21, 2025 at 10:05 PM
🦠🔬 Recently we reported the development of a new class of sensors to directly monitor live-cell protein trafficking and provide a sensitive indication of delivery to functional, enzymatically active lysosomes. A quick 🧵for #FluorescenceFriday www.biorxiv.org/content/10.1...
Fascinating deep-dive into Roche’s new Sequencing By Expansion (SBX) technology
omicsomics.blogspot.com/2025/02/roch...
omicsomics.blogspot.com/2025/02/roch...
Roche Xpounds on New Sequencing Technology
Bar bets can be a powerful force in human society. One of the best known books on the planet, The Guinness Book of World Records, originate...
omicsomics.blogspot.com
February 21, 2025 at 5:45 AM
Fascinating deep-dive into Roche’s new Sequencing By Expansion (SBX) technology
omicsomics.blogspot.com/2025/02/roch...
omicsomics.blogspot.com/2025/02/roch...
Deep-tissue transcriptomics and subcellular imaging at high spatial resolution | Science www.science.org/doi/10.1126/...
Deep-tissue transcriptomics and subcellular imaging at high spatial resolution
Limited color channels in fluorescence microscopy have long constrained spatial analysis in biological specimens. Here, we introduce cycle Hybridization Chain Reaction (HCR), a method that integrates ...
www.science.org
February 21, 2025 at 5:39 AM
Deep-tissue transcriptomics and subcellular imaging at high spatial resolution | Science www.science.org/doi/10.1126/...
A quick photo of lab equipment at 400x: here’s some antibody purification resin after conjugating trastuzumab with Alexa Fluor 594.
February 20, 2025 at 4:41 AM
A quick photo of lab equipment at 400x: here’s some antibody purification resin after conjugating trastuzumab with Alexa Fluor 594.