Chris Langsdorf
@fluorist.bsky.social
I develop fluorescence based dyes and labeling reagents to visualize the complex lives of cells.
Brief, sunny NYC trip, now to Boston for a few days
May 14, 2025 at 12:29 AM
Brief, sunny NYC trip, now to Boston for a few days
Clever “cell floating assay” using CellTrace Violet to sort binders: De novo design of miniprotein agonists and antagonists targeting G protein-coupled receptors
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
April 13, 2025 at 3:29 PM
Clever “cell floating assay” using CellTrace Violet to sort binders: De novo design of miniprotein agonists and antagonists targeting G protein-coupled receptors
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Good afternoon for a jog past the @uoknightcampus.bsky.social
April 7, 2025 at 10:39 PM
Good afternoon for a jog past the @uoknightcampus.bsky.social
Hi @opazo.bsky.social no problem! Here are the antibodies and concentrations used:
April 2, 2025 at 8:14 PM
Hi @opazo.bsky.social no problem! Here are the antibodies and concentrations used:
T shirt and sunglasses day on the mountain
March 25, 2025 at 11:40 PM
T shirt and sunglasses day on the mountain
Spring skiing on Mt Hood
March 24, 2025 at 9:41 PM
Spring skiing on Mt Hood
🌷Spring flowers? Fireworks?
Mouse cerebral cortex FFPE tissue was labeled with eight primary antibodies. Aluora Spatial Amplification Kits and DAPI were used for detection, followed by imaging on the EVOS S1000 Spatial Imaging System.
🧠🔬🧪 #Neuroscience #FluorescenceFriday
Mouse cerebral cortex FFPE tissue was labeled with eight primary antibodies. Aluora Spatial Amplification Kits and DAPI were used for detection, followed by imaging on the EVOS S1000 Spatial Imaging System.
🧠🔬🧪 #Neuroscience #FluorescenceFriday
March 21, 2025 at 5:27 AM
🌷Spring flowers? Fireworks?
Mouse cerebral cortex FFPE tissue was labeled with eight primary antibodies. Aluora Spatial Amplification Kits and DAPI were used for detection, followed by imaging on the EVOS S1000 Spatial Imaging System.
🧠🔬🧪 #Neuroscience #FluorescenceFriday
Mouse cerebral cortex FFPE tissue was labeled with eight primary antibodies. Aluora Spatial Amplification Kits and DAPI were used for detection, followed by imaging on the EVOS S1000 Spatial Imaging System.
🧠🔬🧪 #Neuroscience #FluorescenceFriday
Some microscopy art for St. Patrick's Day. This is antibody purification resin after cleaning up an Alexa Fluor 488 antibody conjugate. 🧪
March 17, 2025 at 8:51 PM
Some microscopy art for St. Patrick's Day. This is antibody purification resin after cleaning up an Alexa Fluor 488 antibody conjugate. 🧪
Three feet of powder means fun in the trees ❄️
March 16, 2025 at 8:25 PM
Three feet of powder means fun in the trees ❄️
For #FluorescenceFriday: a very thin slice of rat kidney was labeled with 8 primary antibodies whose signal was amplified with Aluora Spatial Amplification reagents. 🔬 on the EVOS S1000 Spatial Imaging system. >400 merged fields of view at 20x.
March 15, 2025 at 4:40 AM
For #FluorescenceFriday: a very thin slice of rat kidney was labeled with 8 primary antibodies whose signal was amplified with Aluora Spatial Amplification reagents. 🔬 on the EVOS S1000 Spatial Imaging system. >400 merged fields of view at 20x.
Mountain biking and skiing in the same weekend 🤘
March 10, 2025 at 2:53 AM
Mountain biking and skiing in the same weekend 🤘
Some hummingbirds appear colorful due to iridescence caused by the microscopic structure of their feathers. Here’s an Anna’s hummingbird feather from my yard at 460x 🧪
March 4, 2025 at 1:22 AM
Some hummingbirds appear colorful due to iridescence caused by the microscopic structure of their feathers. Here’s an Anna’s hummingbird feather from my yard at 460x 🧪
Under normal light fluorophore colors appear inverted: red dyes look blue, blue appear red, and green dyes are purple. Using an inverse LUT shows us this same palette on our microscopy sample. 🧪
March 1, 2025 at 9:09 PM
Under normal light fluorophore colors appear inverted: red dyes look blue, blue appear red, and green dyes are purple. Using an inverse LUT shows us this same palette on our microscopy sample. 🧪
Here's a set of three for #FluorescenceFriday. Same image, progressively higher zoom. FFPE human tonsil tissue labeled with primary antibodies against Cytokeratin, PCNA, C20, CD45RB, CD68, eCadherin, and SMA. 🧪🔬
March 1, 2025 at 5:31 AM
Here's a set of three for #FluorescenceFriday. Same image, progressively higher zoom. FFPE human tonsil tissue labeled with primary antibodies against Cytokeratin, PCNA, C20, CD45RB, CD68, eCadherin, and SMA. 🧪🔬
Today in @nature.com: remarkable extended survival in pancreatic cancer patients treated with cancer vaccine.
Autogene cevumeran induces CD8+ T cells with est. lifespan of 7.7 years (range 1.5 to roughly 100 years), with 20% of clones that may outlive hosts.
www.nature.com/articles/s41...
Autogene cevumeran induces CD8+ T cells with est. lifespan of 7.7 years (range 1.5 to roughly 100 years), with 20% of clones that may outlive hosts.
www.nature.com/articles/s41...
February 28, 2025 at 4:35 AM
Today in @nature.com: remarkable extended survival in pancreatic cancer patients treated with cancer vaccine.
Autogene cevumeran induces CD8+ T cells with est. lifespan of 7.7 years (range 1.5 to roughly 100 years), with 20% of clones that may outlive hosts.
www.nature.com/articles/s41...
Autogene cevumeran induces CD8+ T cells with est. lifespan of 7.7 years (range 1.5 to roughly 100 years), with 20% of clones that may outlive hosts.
www.nature.com/articles/s41...
The wetlands outside my office window often has nutria and geese. Today it was a pair of foxes. 🦊
February 25, 2025 at 6:45 PM
The wetlands outside my office window often has nutria and geese. Today it was a pair of foxes. 🦊
These mushrooms are also the source of phalloidin - just as toxic but a useful scientific tool to label the actin cytoskeleton of cells to view under a microscope
February 25, 2025 at 5:05 AM
These mushrooms are also the source of phalloidin - just as toxic but a useful scientific tool to label the actin cytoskeleton of cells to view under a microscope
And more recently we introduced LysoLight Green! Happy imaging and enjoy the weekend.
February 21, 2025 at 10:05 PM
And more recently we introduced LysoLight Green! Happy imaging and enjoy the weekend.
LysoLight is ideally used in live cells; fixation is not recommended. Photostability is excellent, toxicity minimal, enabling long-term kinetic experiments.
February 21, 2025 at 10:05 PM
LysoLight is ideally used in live cells; fixation is not recommended. Photostability is excellent, toxicity minimal, enabling long-term kinetic experiments.
Bright fluorescence is observed upon antigen-specific endosomal trafficking to the lysosome. Activation has been demonstrated with cathepsins B, D, & L. Inhibition is accomplished by blocking cathepsin, vATPase, or saturating cell-surface antigens with competing antibody.
February 21, 2025 at 10:05 PM
Bright fluorescence is observed upon antigen-specific endosomal trafficking to the lysosome. Activation has been demonstrated with cathepsins B, D, & L. Inhibition is accomplished by blocking cathepsin, vATPase, or saturating cell-surface antigens with competing antibody.
The LysoLight family of dyes use a unique sensor that is activated by enzymatic cleavage specific to the lysosome, and therefore reports delivery of a protein or antibody to a functional lysosome. Minimal observable background fluorescence contributes to extraordinary sensitivity.
February 21, 2025 at 10:05 PM
The LysoLight family of dyes use a unique sensor that is activated by enzymatic cleavage specific to the lysosome, and therefore reports delivery of a protein or antibody to a functional lysosome. Minimal observable background fluorescence contributes to extraordinary sensitivity.
Several types of modern biotherapeutics require cellular uptake and lysosome delivery for efficacy. This includes ADCs, IgG-LNP, IgG-oligo, and of course extracellular targeted protein degraders like LYTACs. Existing fluorescence-based sensors report lysosomal delivery, but not catabolic activity.
February 21, 2025 at 10:05 PM
Several types of modern biotherapeutics require cellular uptake and lysosome delivery for efficacy. This includes ADCs, IgG-LNP, IgG-oligo, and of course extracellular targeted protein degraders like LYTACs. Existing fluorescence-based sensors report lysosomal delivery, but not catabolic activity.
A quick photo of lab equipment at 400x: here’s some antibody purification resin after conjugating trastuzumab with Alexa Fluor 594.
February 20, 2025 at 4:41 AM
A quick photo of lab equipment at 400x: here’s some antibody purification resin after conjugating trastuzumab with Alexa Fluor 594.