Filipa Trigo da Roza
@filipatr.bsky.social
Reposted by Filipa Trigo da Roza
This work is finally published! 🥳🧬
Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by
@jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by
@jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Dissecting pOXA-48 fitness effects in clinical Enterobacterales using plasmid-wide CRISPRi screens
Nature Communications - This study investigates the effects of the carbapenem resistance plasmid pOXA-48 in clinical enterobacteria. Using CRISPRi screens, the authors revealed that the...
rdcu.be
August 20, 2025 at 1:25 PM
This work is finally published! 🥳🧬
Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by
@jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by
@jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Reposted by Filipa Trigo da Roza
New paper out! 🔈🔈📣📣
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation.
Combining experimental and computational approaches, we unveil how two of the most prevalent bacterial MGE accelerate the evolution of AMR. 🧵👇🏻
www.biorxiv.org/content/10.1...
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation.
Combining experimental and computational approaches, we unveil how two of the most prevalent bacterial MGE accelerate the evolution of AMR. 🧵👇🏻
www.biorxiv.org/content/10.1...
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation
Antimicrobial Resistance (AMR) is a major threat to public health. Plasmids are mobile genetic elements that can rapidly spread across bacterial populations, promoting the dissemination of AMR genes i...
www.biorxiv.org
August 13, 2025 at 6:26 AM
New paper out! 🔈🔈📣📣
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation.
Combining experimental and computational approaches, we unveil how two of the most prevalent bacterial MGE accelerate the evolution of AMR. 🧵👇🏻
www.biorxiv.org/content/10.1...
Plasmids promote antimicrobial resistance through Insertion Sequence-mediated gene inactivation.
Combining experimental and computational approaches, we unveil how two of the most prevalent bacterial MGE accelerate the evolution of AMR. 🧵👇🏻
www.biorxiv.org/content/10.1...
Reposted by Filipa Trigo da Roza
Our paper “Global dissemination of npmA mediated pan‑aminoglycoside resistance via a mobile element in Gram‑positive bacteria” is now in @natcomms.nature.com. Part of my freshly defended PhD, so doubly happy! 😄🎉
🧵 (1/14)
www.doi.org/10.1038/s414...
🧵 (1/14)
www.doi.org/10.1038/s414...
Global dissemination of npmA mediated pan-aminoglycoside resistance via a mobile genetic element in Gram-positive bacteria - Nature Communications
The authors investigate the distribution of the aminoglycoside resistance gene npmA in Gram-positive bacteria via a mobile genetic element, highlighting its global presence and cross-species transfer ...
www.doi.org
July 17, 2025 at 12:13 PM
Our paper “Global dissemination of npmA mediated pan‑aminoglycoside resistance via a mobile element in Gram‑positive bacteria” is now in @natcomms.nature.com. Part of my freshly defended PhD, so doubly happy! 😄🎉
🧵 (1/14)
www.doi.org/10.1038/s414...
🧵 (1/14)
www.doi.org/10.1038/s414...
Reposted by Filipa Trigo da Roza
Curious about plasmid biology? Our latest paper is out now in Nature Communications! 🚨
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
Universal rules govern plasmid copy number - Nature Communications
Plasmids exhibit a broad range of sizes and copies per cell, and these two parameters appear to be negatively correlated. Here, Ramiro-Martínez et al. analyse the copy number of thousands of diverse b...
doi.org
July 2, 2025 at 10:07 AM
Curious about plasmid biology? Our latest paper is out now in Nature Communications! 🚨
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
doi.org/10.1038/s414...
We analyzed thousands of diverse bacterial plasmids to shed light for the first time on a key aspect of plasmid biology: plasmid copy number. 1/7 👇
Reposted by Filipa Trigo da Roza
Exciting news!! Our latest paper is out in Nat. Microbiol. @natmicrobiol.nature.com
We show that a sub-lineage of 7th pandemic V. cholerae has acquired mobile genetic elements packed with phage defense systems—rendering it multi-phage resistant 😳 ..... 1/3
www.nature.com/articles/s41...
We show that a sub-lineage of 7th pandemic V. cholerae has acquired mobile genetic elements packed with phage defense systems—rendering it multi-phage resistant 😳 ..... 1/3
www.nature.com/articles/s41...
West African–South American pandemic Vibrio cholerae encodes multiple distinct phage defence systems - Nature Microbiology
The West African–South American lineage of Vibrio cholerae contains multiple distinct anti-phage defence systems that provide resistance to various phage families, including vibriophage ICP1, a key pr...
www.nature.com
May 22, 2025 at 6:14 PM
Exciting news!! Our latest paper is out in Nat. Microbiol. @natmicrobiol.nature.com
We show that a sub-lineage of 7th pandemic V. cholerae has acquired mobile genetic elements packed with phage defense systems—rendering it multi-phage resistant 😳 ..... 1/3
www.nature.com/articles/s41...
We show that a sub-lineage of 7th pandemic V. cholerae has acquired mobile genetic elements packed with phage defense systems—rendering it multi-phage resistant 😳 ..... 1/3
www.nature.com/articles/s41...
Reposted by Filipa Trigo da Roza
Reposted by Filipa Trigo da Roza
White smoke, we have a new pope and also 16 new anti-phages systems in sedentary integrons (SCIs) !
In collaboration with the Rocha lab, we show in our new paper that cassettes of these large platforms encode many known anti-phage defenses, and uncovered 16 new ones.
www.science.org/doi/10.1126/...
In collaboration with the Rocha lab, we show in our new paper that cassettes of these large platforms encode many known anti-phage defenses, and uncovered 16 new ones.
www.science.org/doi/10.1126/...
Sedentary chromosomal integrons as biobanks of bacterial antiphage defense systems
Integrons are genetic systems that drive bacterial adaptation by acquiring, expressing, and shuffling gene cassettes. While mobile integrons are well known for spreading antibiotic resistance genes, t...
www.science.org
May 9, 2025 at 11:41 AM
White smoke, we have a new pope and also 16 new anti-phages systems in sedentary integrons (SCIs) !
In collaboration with the Rocha lab, we show in our new paper that cassettes of these large platforms encode many known anti-phage defenses, and uncovered 16 new ones.
www.science.org/doi/10.1126/...
In collaboration with the Rocha lab, we show in our new paper that cassettes of these large platforms encode many known anti-phage defenses, and uncovered 16 new ones.
www.science.org/doi/10.1126/...
Reposted by Filipa Trigo da Roza
🧪
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉
Short 🧵
www.science.org/doi/10.1126/...
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉
Short 🧵
www.science.org/doi/10.1126/...
Mobile integrons encode phage defense systems
Integrons are bacterial genetic elements that capture, stockpile, and modulate the expression of genes encoded in integron cassettes. Mobile integrons (MIs) are borne on plasmids, acting as a vehicle ...
www.science.org
May 8, 2025 at 8:27 PM
🧪
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉
Short 🧵
www.science.org/doi/10.1126/...
Finally out after peer review, our work showing that "Mobile #Integrons carry Phage Defense Systems" is now published in Science 🎉
Short 🧵
www.science.org/doi/10.1126/...
Reposted by Filipa Trigo da Roza
Join the Blokesch lab (@EPFL) as a postdoc in Molecular Microbiology 🤩
Study Vibrio cholerae defense systems (phage/plasmid) in a dynamic, collaborative environment in Lausanne close to Lake Geneva in 🇨🇭!
Please visit my lab's webpage for details about the position and how to formally apply.
Study Vibrio cholerae defense systems (phage/plasmid) in a dynamic, collaborative environment in Lausanne close to Lake Geneva in 🇨🇭!
Please visit my lab's webpage for details about the position and how to formally apply.
April 25, 2025 at 4:15 PM
Join the Blokesch lab (@EPFL) as a postdoc in Molecular Microbiology 🤩
Study Vibrio cholerae defense systems (phage/plasmid) in a dynamic, collaborative environment in Lausanne close to Lake Geneva in 🇨🇭!
Please visit my lab's webpage for details about the position and how to formally apply.
Study Vibrio cholerae defense systems (phage/plasmid) in a dynamic, collaborative environment in Lausanne close to Lake Geneva in 🇨🇭!
Please visit my lab's webpage for details about the position and how to formally apply.
Reposted by Filipa Trigo da Roza
So proud of you, Filipa!! I should have posted something, but I was too sick! We will miss you but you have a brilliant future in front of you! Enjoy it!
February 19, 2025 at 9:57 AM
So proud of you, Filipa!! I should have posted something, but I was too sick! We will miss you but you have a brilliant future in front of you! Enjoy it!
What a ride 🥹
Thank you @jaescudero.bsky.social and the whole MBA lab 💖💖💖
Thank you @jaescudero.bsky.social and the whole MBA lab 💖💖💖
February 18, 2025 at 9:23 PM
What a ride 🥹
Thank you @jaescudero.bsky.social and the whole MBA lab 💖💖💖
Thank you @jaescudero.bsky.social and the whole MBA lab 💖💖💖
Reposted by Filipa Trigo da Roza
Happy International Day of Women and Girls in Science 👩🔬🧬 #womeninscience #girlsinscience
February 11, 2025 at 8:29 AM
Happy International Day of Women and Girls in Science 👩🔬🧬 #womeninscience #girlsinscience
Reposted by Filipa Trigo da Roza
🚨 New preprint! We know that plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by @jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Dissecting pOXA-48 fitness effects in clinical enterobacteria using plasmid-wide CRISPRi screens
Conjugative plasmids are the main vehicle for the spread of antimicrobial resistance (AMR) genes in clinical bacteria. AMR plasmids allow bacteria to survive antibiotic treatments, but they also produ...
www.biorxiv.org
January 24, 2025 at 12:26 PM
🚨 New preprint! We know that plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by @jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Reposted by Filipa Trigo da Roza
Proud supervisor
🎉❤️
🎉❤️
January 23, 2025 at 1:38 PM
Proud supervisor
🎉❤️
🎉❤️
Reposted by Filipa Trigo da Roza
Very happy to see this work published! We unveil a plasmid-chromosome crosstalk by showing that pOXA-48 encodes for a regulator that mediates the transcription of an enterobacterial chromosomal operon, resulting in a growth benefit for the plasmid-carrying bacteria!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
A plasmid-chromosome crosstalk in multidrug resistant enterobacteria - Nature Communications
The authors describe a new crosstalk between a globally disseminated carbapenem resistance plasmid and clinical enterobacteria clones. This crosstalk provides a fitness advantage to the plasmid-carryi...
www.nature.com
January 2, 2025 at 11:20 AM
Very happy to see this work published! We unveil a plasmid-chromosome crosstalk by showing that pOXA-48 encodes for a regulator that mediates the transcription of an enterobacterial chromosomal operon, resulting in a growth benefit for the plasmid-carrying bacteria!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Filipa Trigo da Roza
Exciting times! Our latest preprint is now live 🤩!
After nearly 16 yrs of running my lab, I truly believe this is one of the most significant contributions we've made. It offers fresh hypotheses about how pandemic Vibrio cholerae spreads globally. Let’s dive in!🧵..1/n
www.biorxiv.org/content/10.1...
After nearly 16 yrs of running my lab, I truly believe this is one of the most significant contributions we've made. It offers fresh hypotheses about how pandemic Vibrio cholerae spreads globally. Let’s dive in!🧵..1/n
www.biorxiv.org/content/10.1...
Diverse phage defence systems define West African South American pandemic Vibrio cholerae
Our understanding of the factors underlying the evolutionary success of different lineages of pandemic Vibrio cholerae remains incomplete. Interestingly, two unique genetic signatures define the West ...
www.biorxiv.org
November 25, 2024 at 2:27 PM
Exciting times! Our latest preprint is now live 🤩!
After nearly 16 yrs of running my lab, I truly believe this is one of the most significant contributions we've made. It offers fresh hypotheses about how pandemic Vibrio cholerae spreads globally. Let’s dive in!🧵..1/n
www.biorxiv.org/content/10.1...
After nearly 16 yrs of running my lab, I truly believe this is one of the most significant contributions we've made. It offers fresh hypotheses about how pandemic Vibrio cholerae spreads globally. Let’s dive in!🧵..1/n
www.biorxiv.org/content/10.1...
Reposted by Filipa Trigo da Roza
And its out! New paper for #integron aficionados. We found gene-less cassettes in superintegrons that express/silence other cassettes in the array. Here is a 🧵(if I know how to do this in Bluesky...😆)
academic.oup.com/nar/advance-...
academic.oup.com/nar/advance-...
Identification of promoter activity in gene-less cassettes from Vibrionaceae superintegrons
Abstract. Integrons are genetic platforms that acquire new genes encoded in integron cassettes (ICs), building arrays of adaptive functions. ICs generally encod
academic.oup.com
January 15, 2024 at 7:50 PM
And its out! New paper for #integron aficionados. We found gene-less cassettes in superintegrons that express/silence other cassettes in the array. Here is a 🧵(if I know how to do this in Bluesky...😆)
academic.oup.com/nar/advance-...
academic.oup.com/nar/advance-...
Reposted by Filipa Trigo da Roza
Check out our last work with Celine Loot, Ole Skovgaard, @amazeld.bsky.social and @sanmillan.bsky.social. We have deleted the Superintegron from V. cholerae! A long-awaited feat in the field!! Despite losing 3% of the genome, the strain remains phenotipically identical across Manu conditions!! 😵
Chromosomal Integrons are Genetically and Functionally Isolated Units of Genomes
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
November 21, 2023 at 1:22 PM
Check out our last work with Celine Loot, Ole Skovgaard, @amazeld.bsky.social and @sanmillan.bsky.social. We have deleted the Superintegron from V. cholerae! A long-awaited feat in the field!! Despite losing 3% of the genome, the strain remains phenotipically identical across Manu conditions!! 😵