· Changes in floral morphology are key for the evolutionary success of flowering plants. However, many species-rich and ecologically diverse groups show low variation in floral traits. Low morphologic...

Motivation: Long-Read Sequencing (LRS) promises great improvements in the detection of structural genome variants (SVs). However, existing methods are lacking in key areas such as the reliable detecti...

Transcription factors (TFs) are traditionally depicted as monomeric, or oligomeric, units that recognize and bind specific DNA sequences to regulate transcription. Emerging evidence suggests that many TFs can undergo liquid phase separation, resulting in condensates that bind DNA through a different mechanism. For the Auxin Response Factors (ARFs), canonical plant TFs, evidence for both scenarios exists. But which of these scenarios is operational in the plant nucleus is unclear. Here, we demonstrate using MpARF2 of Marchantia polymorpha that a third scenario is operational: MpARF2 forms nanoscopic clusters under physiological conditions. Nanoclusters combine DNA-binding features that cannot be accessed by the other two scenarios: high-affinity, switch-like, and sequence-specific. Our results suggest nanoclustering as a mechanism that equips TFs with the DNA-binding properties necessary for transcriptional regulation. ### Competing Interest Statement The authors have declared no competing interest. European Research Council, Catch 101000981 European Research Council, DIRNDL 833867 Human Frontiers Science Program, RGP0015/2022 Marie Skłodowska-Curie Individual Fellowship 2020, REOX 101026004 NWO, 184.036.012

Pepper (Capsicum) is among the most widely cultivated and consumed vegetable crops worldwide. Although extensive studies in model fruit crops such as tomato have provided insights into the genetic and...

The flowering plant order Asterales exhibits a striking disparity in species richness, with >30,000 species in Asteraceae compared to <50 in its sister family Calyceraceae. To investigate the genomic ...

Cowpea (Vigna unguiculata L. Walp) is a dryland legume crop, providing essential food and nutritional security for millions of people across the semi-arid tropics, in Africa, Asia, and Latin America. ...

Understanding how mutations affect protein function remains critical yet challenging, particularly for variants in clinical databases lacking experimental characterisation and for intrinsically disord...

Omic technologies revolutionised research in microbiomics and helped decipher the complex community structure of gut microbiota, each comprising hundreds of species of bacteria, archaea, and protists. Even as sequencing costs decrease, understanding these communities remains a challenge. Here, we introduce annoSnake, a Snakemake workflow that facilitates taxonomic and functional annotation of metagenomes and metagenomic-assembled genomes (MAGs). This user-friendly and highly flexible workflow integrates state-of-the-art software packages, taking raw sequencing reads, assembling them into contigs, assigning taxonomies and annotating them using various popular protein databases. The workflow handles paired-end as well as interleaved read data, and provides publication-ready figures and tables for downstream analysis. annoSnake, as all Snakemake workflows, is highly scalable, reproducible, and portable, enabling execution across diverse high-performance computing environments with remarkable efficiency. ### Competing Interest Statement The authors have declared no competing interest.

Symbioses have been fundamental to colonization of terrestrial ecosystems by plants and their evolution. Emergence of the ancient arbuscular mycorrhizal symbiosis was followed by the diversification o...

Arbuscular mycorrhizal (AM) symbiosis develops through successive colonization of root epidermal and cortical cells, culminating in the formation of arbuscules, tree-like intracellular structures that...

Nanopore direct RNA sequencing (DRS) coupled with Dorado modification-aware basecalling enables mapping of epitranscriptomic modifications including N6-methyladenosine (m6A) at the level of individual...

Background Experimental proof of gene function assignments in plants is based on mutant analyses. T-DNA insertion lines provided an invaluable resource of mutants and enabled systematic reverse geneti...

Despite the ongoing increase in atmospheric carbon dioxide concentrations, these levels remain well below the optimal threshold for cucumber growth and development. In addressing the challenges posed ...

Plants encounter diverse pathogens and have evolved a two-layered innate immune system to detect pathogen molecules and activate defense mechanisms that restrict infection. Most cloned plant Resistanc...