Stephen Bell
@bellp450enzyme.bsky.social
Chemist, heme enzyme enthusiast, cytochrome P450 tinkerer.
I enjoyed contributing to this article from the @jarworrall.bsky.social lab at the @universityofessex.bsky.social during a visit over their summer. A first foray for me into P450 peroxygenase fatty acid decarboxylases to make alkenes from fatty acids.
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
The cytochrome P450 decarboxylase from Staphylococcus aureus can produce a diene from a C18 monounsaturated fatty acid: A spectroscopic, structural and kinetic characterisation
Certain members of the bacterial cytochrome P450 152 family (CYP152) are peroxygenases that catalyse the decarboxylation of fatty acids into terminal …
www.sciencedirect.com
October 21, 2025 at 10:08 PM
I enjoyed contributing to this article from the @jarworrall.bsky.social lab at the @universityofessex.bsky.social during a visit over their summer. A first foray for me into P450 peroxygenase fatty acid decarboxylases to make alkenes from fatty acids.
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Stephen Bell
Our first foray into the P450 world. Thanks to @bellp450enzyme.bsky.social for sharpening our GC-MS skill set @jamesbirrell.bsky.social for the microwaves😃 and Lewis for persevering 🧪
The cytochrome P450 decarboxylase from Staphylococcus aureus can produce a diene from a C18 monounsaturated fatty acid: A spectroscopic, structural and kinetic characterisation
Certain members of the bacterial cytochrome P450 152 family (CYP152) are peroxygenases that catalyse the decarboxylation of fatty acids into terminal …
www.sciencedirect.com
October 21, 2025 at 8:45 PM
Our first foray into the P450 world. Thanks to @bellp450enzyme.bsky.social for sharpening our GC-MS skill set @jamesbirrell.bsky.social for the microwaves😃 and Lewis for persevering 🧪
Characterisation of a Self‐Sufficient Cytochrome P450 Enzyme From the Bacterium Thermosporothrix hazakensis and Its Conversion Into a Peroxygenase - Podgorski - 2025 - Microbial Biotechnology - Wiley Online Library enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/...
October 3, 2025 at 11:54 AM
Characterisation of a Self‐Sufficient Cytochrome P450 Enzyme From the Bacterium Thermosporothrix hazakensis and Its Conversion Into a Peroxygenase - Podgorski - 2025 - Microbial Biotechnology - Wiley Online Library enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/...
Reposted by Stephen Bell
A really engaging TIBS Spotlight article highlighting our recent structures of a cytochrome P450 SyoA. Thanks to Professor Jennifer DuBois for elegantly contextualising the work within the broader field of lignin valorisation. Upcycling lignin with a controlled burn tinyurl.com/2s3w9ej6 🔥🫰
Upcycling lignin with a controlled burn
A recent report by Harlington et al. introduces SyoA, a cytochrome P450 enzyme that
efficiently removes a methyl group from the S-subunits of lignin. Lignin is one of
the most abundant forms of renewa...
tinyurl.com
June 16, 2025 at 11:01 AM
A really engaging TIBS Spotlight article highlighting our recent structures of a cytochrome P450 SyoA. Thanks to Professor Jennifer DuBois for elegantly contextualising the work within the broader field of lignin valorisation. Upcycling lignin with a controlled burn tinyurl.com/2s3w9ej6 🔥🫰
Reposted by Stephen Bell
Primary @natcomms.nature.com article: "Structural insights into S-lignin O-demethylation via a rare class of heme peroxygenase enzymes" from @bellp450enzyme.bsky.social, @freewhelan.bsky.social and colleagues.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structural insights into S-lignin O-demethylation via a rare class of heme peroxygenase enzymes - Nature Communications
This article demonstrates that native P450 peroxygenases with the in-built machinery for hydrogen peroxide utilisation exist; defines the molecular template for native P450 peroxygenase activity; and ...
www.nature.com
June 12, 2025 at 3:32 PM
Primary @natcomms.nature.com article: "Structural insights into S-lignin O-demethylation via a rare class of heme peroxygenase enzymes" from @bellp450enzyme.bsky.social, @freewhelan.bsky.social and colleagues.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Stephen Bell
Online now - the Spotlight "Upcycling #lignin with a controlled burn" from Jennifer DuBois.
#CYPenzymes #CytochromeP450 #Biocatalysis #Bioreactor
Read it here: authors.elsevier.com/a/1lFS93S6Gf...
#CYPenzymes #CytochromeP450 #Biocatalysis #Bioreactor
Read it here: authors.elsevier.com/a/1lFS93S6Gf...
June 12, 2025 at 3:32 PM
Online now - the Spotlight "Upcycling #lignin with a controlled burn" from Jennifer DuBois.
#CYPenzymes #CytochromeP450 #Biocatalysis #Bioreactor
Read it here: authors.elsevier.com/a/1lFS93S6Gf...
#CYPenzymes #CytochromeP450 #Biocatalysis #Bioreactor
Read it here: authors.elsevier.com/a/1lFS93S6Gf...
Upcycling lignin with a controlled burn: Trends in Biochemical Sciences www.cell.com/trends/bioch...
Upcycling lignin with a controlled burn
A recent report by Harlington et al. introduces SyoA, a cytochrome P450 enzyme that
efficiently removes a methyl group from the S-subunits of lignin. Lignin is one of
the most abundant forms of renewa...
www.cell.com
June 13, 2025 at 8:43 AM
Upcycling lignin with a controlled burn: Trends in Biochemical Sciences www.cell.com/trends/bioch...
New paper investigating a new #biocatalyst from a #metagenome
Characterisation of the #thermophilic #P450 CYP116B305 identified using metagenomics-derived sequence data from an Australian hot spring. Appl Microbiol Biotechnol 109, 133 (2025). doi.org/10.1007/s002...
Characterisation of the #thermophilic #P450 CYP116B305 identified using metagenomics-derived sequence data from an Australian hot spring. Appl Microbiol Biotechnol 109, 133 (2025). doi.org/10.1007/s002...
Characterisation of the thermophilic P450 CYP116B305 identified using metagenomics-derived sequence data from an Australian hot spring - Applied Microbiology and Biotechnology
Abstract Cytochrome P450 enzymes (P450s) have gained significant attention due to their remarkable ability to oxidise unactivated C-H bonds with high regio- and stereoselectivity. Their industrial uti...
doi.org
June 1, 2025 at 11:53 AM
New paper investigating a new #biocatalyst from a #metagenome
Characterisation of the #thermophilic #P450 CYP116B305 identified using metagenomics-derived sequence data from an Australian hot spring. Appl Microbiol Biotechnol 109, 133 (2025). doi.org/10.1007/s002...
Characterisation of the #thermophilic #P450 CYP116B305 identified using metagenomics-derived sequence data from an Australian hot spring. Appl Microbiol Biotechnol 109, 133 (2025). doi.org/10.1007/s002...
α-Synuclein and Dopamine Metabolites DOPAL and DOPAC: A Pathway to New Synthetic Neuromelanin Models sciltp.com/journals/bb/...
First article in the new journal Bioinorganics and Biocatalysis
@scilightpress.bsky.social
First article in the new journal Bioinorganics and Biocatalysis
@scilightpress.bsky.social
α-Synuclein and Dopamine Metabolites DOPAL and DOPAC: A Pathway to New Synthetic Neuromelanin Models
Melanin-protein-Fe conjugates serving as new synthetic analogues of brain neuromelanin were prepared by oxidative oligomerization of dopamine (DA) and its metabolites, 3,4-dihydroxyphenyl acetaldehyde...
sciltp.com
May 17, 2025 at 11:24 AM
α-Synuclein and Dopamine Metabolites DOPAL and DOPAC: A Pathway to New Synthetic Neuromelanin Models sciltp.com/journals/bb/...
First article in the new journal Bioinorganics and Biocatalysis
@scilightpress.bsky.social
First article in the new journal Bioinorganics and Biocatalysis
@scilightpress.bsky.social
New paper in chemical science on #p450 #sterol oxidation.
@chemicalscience.rsc.org
doi.org/10.1039/D5SC...
@chemicalscience.rsc.org
doi.org/10.1039/D5SC...
Evolutionary insights into the selectivity of sterol oxidising cytochrome P450 enzymes based on ancestral sequence reconstruction.
The cytochrome P450 (CYP) enzyme CYP125A1 is a crucial enzyme for the long-term survival and pathogenicity of Mycobacterium tuberculosis. CYP125 genes are found not only in pathogenic mycobacteria but...
doi.org
May 14, 2025 at 8:01 PM
New paper in chemical science on #p450 #sterol oxidation.
@chemicalscience.rsc.org
doi.org/10.1039/D5SC...
@chemicalscience.rsc.org
doi.org/10.1039/D5SC...
Characterisation of the Self‐Sufficient Cytochrome P450 CYP116B234 From Rhodococcus globerulus and Its Suggested Native Role in 2‐Hydroxyphenylacetic Acid Metabolism doi.org/10.1111/1751...
Characterisation of the Self‐Sufficient Cytochrome P450 CYP116B234 From Rhodococcus globerulus and Its Suggested Native Role in 2‐Hydroxyphenylacetic Acid Metabolism
A self-sufficient P450 gene (CYP116B234) was identified from Rhodococcus globerulus, a soil-dwelling bacterium isolated from beneath an Eucalypt tree. CYP116B234 consists of heme and reductase domain...
doi.org
March 13, 2025 at 12:11 AM
Characterisation of the Self‐Sufficient Cytochrome P450 CYP116B234 From Rhodococcus globerulus and Its Suggested Native Role in 2‐Hydroxyphenylacetic Acid Metabolism doi.org/10.1111/1751...
Pleased to see this excellent and comprehensive piece of #enzyme #biocatalysis work from finishing PhD student Matthew Podgorski from Uni of Adelaide
Comparing and Combining Alternative Strategies for Enhancing Cytochrome P450 Peroxygenase Activity | ACS Catalysis pubs.acs.org/doi/10.1021/...
Comparing and Combining Alternative Strategies for Enhancing Cytochrome P450 Peroxygenase Activity | ACS Catalysis pubs.acs.org/doi/10.1021/...
Comparing and Combining Alternative Strategies for Enhancing Cytochrome P450 Peroxygenase Activity
Cytochrome P450 monooxygenase (CYP) enzymes have advantageous properties over chemical catalysts. However, it is often not feasible to use CYPs in larger-scale synthesis as they require additional cof...
pubs.acs.org
March 13, 2025 at 12:04 AM
Pleased to see this excellent and comprehensive piece of #enzyme #biocatalysis work from finishing PhD student Matthew Podgorski from Uni of Adelaide
Comparing and Combining Alternative Strategies for Enhancing Cytochrome P450 Peroxygenase Activity | ACS Catalysis pubs.acs.org/doi/10.1021/...
Comparing and Combining Alternative Strategies for Enhancing Cytochrome P450 Peroxygenase Activity | ACS Catalysis pubs.acs.org/doi/10.1021/...
Reposted by Stephen Bell
Happy to report our enzyme found a home! rdcu.be/eaGXG New kid on the block P450 O-demethylase SyoA-the specificity of a monooxygenase with the simplicity of catalysis driven by peroxide. Retrofit your P450 with this active site chemistry today! #lignin #syringol Out today in Nature Communications.
February 21, 2025 at 12:17 AM
Happy to report our enzyme found a home! rdcu.be/eaGXG New kid on the block P450 O-demethylase SyoA-the specificity of a monooxygenase with the simplicity of catalysis driven by peroxide. Retrofit your P450 with this active site chemistry today! #lignin #syringol Out today in Nature Communications.
Reposted by Stephen Bell
Congratulations to PhD candidate @alixharlington.bsky.social, the driving force behind this research; and thanks to @bellp450enzyme.bsky.social for an enjoyable and productive collaboration. Thanks also to Keith Shearwin for your unflagging support & Tuhin Das for your work characterising mutants.
February 21, 2025 at 12:24 AM
Congratulations to PhD candidate @alixharlington.bsky.social, the driving force behind this research; and thanks to @bellp450enzyme.bsky.social for an enjoyable and productive collaboration. Thanks also to Keith Shearwin for your unflagging support & Tuhin Das for your work characterising mutants.
Reposted by Stephen Bell
Thanks to the #ARC and Ramsay fellowship scheme for supporting this research, and to the #AustralianSynchrotron MX beamline staff for keeping our critical infrastructure accessible and productive!
February 21, 2025 at 12:54 AM
Thanks to the #ARC and Ramsay fellowship scheme for supporting this research, and to the #AustralianSynchrotron MX beamline staff for keeping our critical infrastructure accessible and productive!
Characterisation of the Cytochrome P450 Monooxygenase CYP116B46 from Tepidiphilus thermophilus as a Homogentisic Acid Generating Enzyme and its Conversion to a Peroxygenase - Akter - ChemBioChem - Wiley Online Library chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
January 22, 2025 at 7:46 AM
Characterisation of the Cytochrome P450 Monooxygenase CYP116B46 from Tepidiphilus thermophilus as a Homogentisic Acid Generating Enzyme and its Conversion to a Peroxygenase - Akter - ChemBioChem - Wiley Online Library chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...