We developed a glycoproteomics workflow (Fig. 1a) using commercially available, economical silica beads functionalized with PBA to selectively enrich intact glycopeptides. All steps of the workflow, from sample preparation to glycopeptide enrichment, were performed in 96-well (filter) plates, increasing sample...

Here the authors present deep quantitative glycoprofiling (DQGlyco), a method that enables high-throughput analysis of protein glycosylation dynamics. Using DQGlyco, they link gut microbiome compositi...