Plants counter with NLRs R2 & Rpi-mcq1, which guard both the pH regulator (StATP6V1C1) and its downstream target — a dual-layer decoy surveillance system.
A new arms race battlefield: intracellular pH homeostasis.
#PlantImmunity #Oomycete #LateBlight #PlantPathology

Experimental validation proves the power of AI:
Our synthetic enhancers achieved an 11.4x higher success rate than traditional methods. The top performer, STEsyn3578, reached an activity level of 64.5x—surpassing its natural counterpart by 2.27x and breaking the limits of natural evolution. 🚀🧬

🚀 AI vs. Sequence Space:Designing a 60-bp enhancer means navigating 1.33 × 10^36 possibilities. BaseSearch AI solves this!
Efficiency: Boosted by 27.8% via evolutionary strategies.
Results: 5,000 synthetic enhancers designed "on-demand." Impact: Moving beyond nature to engineer the future of crops.

Platform: STEM-seq for high-throughput, position-independent screening.
Scale: 81,475 elements screened; 6,904 functional STEs identified.
Precision: At just 60-80 bp, these enhancers are ideal "genetic switches" for CRISPR editing in major crops like maize, wheat, and soybean. 🚀

This isn't just a lab win. 🏆 This CRISPR-Act platform provides a robust toolkit for tea breeders. Instead of waiting decades for natural mutations, we can now precisely optimize secondary metabolites. The era of high-performance, designer tea varieties is officially here! 🚀🍃 #Innovation #Futurebreed

Why L-theanine? It’s the "magic molecule" in tea that provides that unique umami flavor and calming effect. 🧘‍♂️ By precision-tuning the expression of biosynthetic enzymes, this study successfully increased theanine levels, paving the way for "Functional Tea" that tastes better and relaxes more. 🧬