Claire Masson
@wclairemasson.bsky.social
PhD Student UMR_S1255
🔬 Electron microscopy and fluorescence enthusiast - in situ imaging of bone marrow, especially the incredible #megakaryocyte in #cancer
📍Strasbourg, France
🔬 Electron microscopy and fluorescence enthusiast - in situ imaging of bone marrow, especially the incredible #megakaryocyte in #cancer
📍Strasbourg, France
7/
Want to dive deeper? 🧠
Read the full story here 👉
elifesciences.org/articles/104...
Huge thanks to all co-authors and collaborators for this amazing scientific adventure 🙏
@renaudpoincloux.bsky.social @goetzjacky.bsky.social @naelosmani.bsky.social
Always happy to discuss!
Want to dive deeper? 🧠
Read the full story here 👉
elifesciences.org/articles/104...
Huge thanks to all co-authors and collaborators for this amazing scientific adventure 🙏
@renaudpoincloux.bsky.social @goetzjacky.bsky.social @naelosmani.bsky.social
Always happy to discuss!
Megakaryocytes assemble a three-dimensional cage of extracellular matrix that controls their maturation and anchoring to the vascular niche
Megakaryocytes form a 3D cage of laminin and collagen IV that stabilizes them within their vascular niche, while β1/β3 integrins and MMP are key regulators that assist megakaryocyte maturation and int...
elifesciences.org
October 9, 2025 at 7:48 PM
7/
Want to dive deeper? 🧠
Read the full story here 👉
elifesciences.org/articles/104...
Huge thanks to all co-authors and collaborators for this amazing scientific adventure 🙏
@renaudpoincloux.bsky.social @goetzjacky.bsky.social @naelosmani.bsky.social
Always happy to discuss!
Want to dive deeper? 🧠
Read the full story here 👉
elifesciences.org/articles/104...
Huge thanks to all co-authors and collaborators for this amazing scientific adventure 🙏
@renaudpoincloux.bsky.social @goetzjacky.bsky.social @naelosmani.bsky.social
Always happy to discuss!
6/
⚖️ So, the balance of ECM remodeling is key:
Too loose → MKs escape.
Too tight → MKs can’t mature.
The ECM cage is a dynamic scaffold that fine-tunes megakaryocyte function and platelet formation.
⚖️ So, the balance of ECM remodeling is key:
Too loose → MKs escape.
Too tight → MKs can’t mature.
The ECM cage is a dynamic scaffold that fine-tunes megakaryocyte function and platelet formation.
October 9, 2025 at 7:48 PM
6/
⚖️ So, the balance of ECM remodeling is key:
Too loose → MKs escape.
Too tight → MKs can’t mature.
The ECM cage is a dynamic scaffold that fine-tunes megakaryocyte function and platelet formation.
⚖️ So, the balance of ECM remodeling is key:
Too loose → MKs escape.
Too tight → MKs can’t mature.
The ECM cage is a dynamic scaffold that fine-tunes megakaryocyte function and platelet formation.
5/🦴
What if we make the cage too dense?
When we inhibit matrix metalloproteinases (MMPs), the ECM cage becomes denser — and MKs fail to mature properly.
Result: more small, immature MKs accumulate in the bone marrow.
What if we make the cage too dense?
When we inhibit matrix metalloproteinases (MMPs), the ECM cage becomes denser — and MKs fail to mature properly.
Result: more small, immature MKs accumulate in the bone marrow.
October 9, 2025 at 7:48 PM
5/🦴
What if we make the cage too dense?
When we inhibit matrix metalloproteinases (MMPs), the ECM cage becomes denser — and MKs fail to mature properly.
Result: more small, immature MKs accumulate in the bone marrow.
What if we make the cage too dense?
When we inhibit matrix metalloproteinases (MMPs), the ECM cage becomes denser — and MKs fail to mature properly.
Result: more small, immature MKs accumulate in the bone marrow.
4/
But why build a cage around MKs? 🤔
We used mice with integrin β1/β3 knocked out specifically in MKs and found that these receptors are essential for cage formation.
Without them, MKs lose their anchorage — and some even enter the bloodstream intact!
➡️ Integrins keep MKs grounded.
But why build a cage around MKs? 🤔
We used mice with integrin β1/β3 knocked out specifically in MKs and found that these receptors are essential for cage formation.
Without them, MKs lose their anchorage — and some even enter the bloodstream intact!
➡️ Integrins keep MKs grounded.
October 9, 2025 at 7:48 PM
4/
But why build a cage around MKs? 🤔
We used mice with integrin β1/β3 knocked out specifically in MKs and found that these receptors are essential for cage formation.
Without them, MKs lose their anchorage — and some even enter the bloodstream intact!
➡️ Integrins keep MKs grounded.
But why build a cage around MKs? 🤔
We used mice with integrin β1/β3 knocked out specifically in MKs and found that these receptors are essential for cage formation.
Without them, MKs lose their anchorage — and some even enter the bloodstream intact!
➡️ Integrins keep MKs grounded.
3/ 🔬
Using high-resolution 3D imaging, we mapped ECM proteins around MKs.
Surprisingly, laminin and collagen IV form a 3D cage that completely surrounds MKs and connects to the sinusoidal basement membrane.
👉 This “ECM cage” stabilizes MKs in their vascular niche.
Using high-resolution 3D imaging, we mapped ECM proteins around MKs.
Surprisingly, laminin and collagen IV form a 3D cage that completely surrounds MKs and connects to the sinusoidal basement membrane.
👉 This “ECM cage” stabilizes MKs in their vascular niche.
October 9, 2025 at 7:48 PM
3/ 🔬
Using high-resolution 3D imaging, we mapped ECM proteins around MKs.
Surprisingly, laminin and collagen IV form a 3D cage that completely surrounds MKs and connects to the sinusoidal basement membrane.
👉 This “ECM cage” stabilizes MKs in their vascular niche.
Using high-resolution 3D imaging, we mapped ECM proteins around MKs.
Surprisingly, laminin and collagen IV form a 3D cage that completely surrounds MKs and connects to the sinusoidal basement membrane.
👉 This “ECM cage” stabilizes MKs in their vascular niche.
2/
We know that the ECM is a major component of the bone marrow, especially in the basement membrane.
But what is its role in megakaryocyte maturation and platelet production?
💡 Spoiler: ECM and MK work hand in hand to ensure efficient platelet release.
We know that the ECM is a major component of the bone marrow, especially in the basement membrane.
But what is its role in megakaryocyte maturation and platelet production?
💡 Spoiler: ECM and MK work hand in hand to ensure efficient platelet release.
a man wearing a crown is talking to another man wearing a party hat
ALT: a man wearing a crown is talking to another man wearing a party hat
media.tenor.com
October 9, 2025 at 7:48 PM
2/
We know that the ECM is a major component of the bone marrow, especially in the basement membrane.
But what is its role in megakaryocyte maturation and platelet production?
💡 Spoiler: ECM and MK work hand in hand to ensure efficient platelet release.
We know that the ECM is a major component of the bone marrow, especially in the basement membrane.
But what is its role in megakaryocyte maturation and platelet production?
💡 Spoiler: ECM and MK work hand in hand to ensure efficient platelet release.
1/ 👩🔬The megakaryocyte (MK) is a fascinating giant cell that produces platelets in the bone marrow.
Situated near sinusoids, it extends long cytoplasmic protrusions that cross the vessel wall — these fragment into platelets, while the nucleus stays in the stroma.
Situated near sinusoids, it extends long cytoplasmic protrusions that cross the vessel wall — these fragment into platelets, while the nucleus stays in the stroma.
October 9, 2025 at 7:48 PM
1/ 👩🔬The megakaryocyte (MK) is a fascinating giant cell that produces platelets in the bone marrow.
Situated near sinusoids, it extends long cytoplasmic protrusions that cross the vessel wall — these fragment into platelets, while the nucleus stays in the stroma.
Situated near sinusoids, it extends long cytoplasmic protrusions that cross the vessel wall — these fragment into platelets, while the nucleus stays in the stroma.