Dr. Peter Walentek (Walentek Lab)
@walenteklab.bsky.social
(he/him) Cilia & mucociliary epithelia development, self-organization, and human airway diseases. 🏳️🌈 opinions own & plenty.
Lab link: uniklinik-freiburg.de/walentek-en.html
Lab link: uniklinik-freiburg.de/walentek-en.html
Congrats to Henrike! Not only she has published her WNT11 study in DMM, but she also got the cover and now was selected for the Biomedical picture of the day!
bpod.org.uk
👏👏👏 the image was taken at the 2024 @cshlxeno course btw. #xenopus 👊
bpod.org.uk
👏👏👏 the image was taken at the 2024 @cshlxeno course btw. #xenopus 👊
June 18, 2025 at 12:28 PM
Congrats to Henrike! Not only she has published her WNT11 study in DMM, but she also got the cover and now was selected for the Biomedical picture of the day!
bpod.org.uk
👏👏👏 the image was taken at the 2024 @cshlxeno course btw. #xenopus 👊
bpod.org.uk
👏👏👏 the image was taken at the 2024 @cshlxeno course btw. #xenopus 👊
Save the date 📅! Our TriNational #devbio #stemcell Meeting 2025 will be held in Freiburg on Sept. 19th. As always, participation is free and talks will be selected from early career scientists. Joun us 😊
Info: devstemcell.org/home
@biozentrum.unibas.ch @uni-freiburg.de @igbmc.bsky.social
Info: devstemcell.org/home
@biozentrum.unibas.ch @uni-freiburg.de @igbmc.bsky.social
June 16, 2025 at 6:44 AM
Save the date 📅! Our TriNational #devbio #stemcell Meeting 2025 will be held in Freiburg on Sept. 19th. As always, participation is free and talks will be selected from early career scientists. Joun us 😊
Info: devstemcell.org/home
@biozentrum.unibas.ch @uni-freiburg.de @igbmc.bsky.social
Info: devstemcell.org/home
@biozentrum.unibas.ch @uni-freiburg.de @igbmc.bsky.social
Finally spring... 🥳
May 30, 2025 at 8:21 PM
Finally spring... 🥳
Huge congratulations to Dr. Magdalena Brislinger-Engelhardt for defending her PhD thesis today 👏👏👏🥳🥳🥳
May 28, 2025 at 4:05 PM
Huge congratulations to Dr. Magdalena Brislinger-Engelhardt for defending her PhD thesis today 👏👏👏🥳🥳🥳
We have extended the deadline until Feb. 14th. Take the opportunity, funding support is avaliable! 🐸⚠️
meetings.cshl.edu/courses.aspx...
meetings.cshl.edu/courses.aspx...
February 5, 2025 at 8:51 PM
We have extended the deadline until Feb. 14th. Take the opportunity, funding support is avaliable! 🐸⚠️
meetings.cshl.edu/courses.aspx...
meetings.cshl.edu/courses.aspx...
...and last but nit least, we'll have intros on services by #Xenopus resource centers around the 🌎 @xenbase.bsky.social #exrc #nxr #japaneseamphibiancenter ....
And plenty of financial support available!
Join us, it's gonna be a blast 🐸✊️
And plenty of financial support available!
Join us, it's gonna be a blast 🐸✊️
January 22, 2025 at 11:32 AM
...and last but nit least, we'll have intros on services by #Xenopus resource centers around the 🌎 @xenbase.bsky.social #exrc #nxr #japaneseamphibiancenter ....
And plenty of financial support available!
Join us, it's gonna be a blast 🐸✊️
And plenty of financial support available!
Join us, it's gonna be a blast 🐸✊️
And we'll have awesome scopes 🔬for use from companies.... see one beautiful example 🐸👁 in a transgenic brainbow line induced by heatshock-Cre 😍
January 22, 2025 at 11:29 AM
And we'll have awesome scopes 🔬for use from companies.... see one beautiful example 🐸👁 in a transgenic brainbow line induced by heatshock-Cre 😍
We will have a bunch 9f techniques covered
January 22, 2025 at 11:26 AM
We will have a bunch 9f techniques covered
Rachel Miller and I got fantastic course TAs ready, and there will be a whole lot of great diverse faculty teaching various aspects of cell, dev, genetics/genomics, regeneration, mechanobiology etc topics. 🤩
January 22, 2025 at 11:25 AM
Rachel Miller and I got fantastic course TAs ready, and there will be a whole lot of great diverse faculty teaching various aspects of cell, dev, genetics/genomics, regeneration, mechanobiology etc topics. 🤩
Join us for the fantastic annual @cshlnews.bsky.social #Xenopus cell & developmental Biology course.
Deadline for application is Jan 31st.
meetings.cshl.edu/courses.aspx...
Deadline for application is Jan 31st.
meetings.cshl.edu/courses.aspx...
January 22, 2025 at 11:22 AM
Join us for the fantastic annual @cshlnews.bsky.social #Xenopus cell & developmental Biology course.
Deadline for application is Jan 31st.
meetings.cshl.edu/courses.aspx...
Deadline for application is Jan 31st.
meetings.cshl.edu/courses.aspx...
But why? Henrike checked dimerization ability and protein levels. This revealed that the variant had reduced stability and levels leading to lack of signaling activity... this was restored by changing the last amino acids.
November 17, 2024 at 4:45 PM
But why? Henrike checked dimerization ability and protein levels. This revealed that the variant had reduced stability and levels leading to lack of signaling activity... this was restored by changing the last amino acids.
Next we used #xenopus #wnt reporters from @krisvleminckx.bsky.social and indeed Henrike generated a dominant-negative acting human WNT11 by shortening the ligand.
November 17, 2024 at 4:44 PM
Next we used #xenopus #wnt reporters from @krisvleminckx.bsky.social and indeed Henrike generated a dominant-negative acting human WNT11 by shortening the ligand.
Deleting these from dnWnt11b made it a but weaker... but still dominant-negative.
November 17, 2024 at 4:44 PM
Deleting these from dnWnt11b made it a but weaker... but still dominant-negative.
AND when we looked at left right development... niw it acted just like dnWnt11b!
November 17, 2024 at 4:43 PM
AND when we looked at left right development... niw it acted just like dnWnt11b!
🤯 deleting or correcting just 12 amino acids restored dominant-negative function in the allel when we looked at gastrulation...
November 17, 2024 at 4:43 PM
🤯 deleting or correcting just 12 amino acids restored dominant-negative function in the allel when we looked at gastrulation...
Henrike compared sequences and lengths, and generated constructs that deleted missense amino acids or corrected them in the truncated WNT11s... she also altered the known dnWnt11b construct from xenopus... is the loss of function due to length, sequence or what...???
November 17, 2024 at 4:42 PM
Henrike compared sequences and lengths, and generated constructs that deleted missense amino acids or corrected them in the truncated WNT11s... she also altered the known dnWnt11b construct from xenopus... is the loss of function due to length, sequence or what...???
While the new variant did not alter normal expression on the left, and dnWnt11b inhibited expression on both sides (as we have shown many years ago). So the variant seems to be a loss of function allel...
November 17, 2024 at 4:41 PM
While the new variant did not alter normal expression on the left, and dnWnt11b inhibited expression on both sides (as we have shown many years ago). So the variant seems to be a loss of function allel...
When we look at left-right asymmetry gene expression. Overexpression of functional Wnt11s induces bilateral expression...
November 17, 2024 at 4:41 PM
When we look at left-right asymmetry gene expression. Overexpression of functional Wnt11s induces bilateral expression...
We overexpressed 👨 and 🐸 Wnt11, the new variant and dnWnt11b and assessed morphology in #Xenopus. Indeed, the new variant did not interfere with gastrulation...
November 17, 2024 at 4:40 PM
We overexpressed 👨 and 🐸 Wnt11, the new variant and dnWnt11b and assessed morphology in #Xenopus. Indeed, the new variant did not interfere with gastrulation...
But both parents were heterozygous carriers without heart and kidney defects. This argued against a dominant-negative function. And Henrike identified small differences...
November 17, 2024 at 4:40 PM
But both parents were heterozygous carriers without heart and kidney defects. This argued against a dominant-negative function. And Henrike identified small differences...
The c-terminal trunkation in the ligand made it look like dominant-negative Wnt11b from Xenopus, where laterality, heart and kidney defects were described before after loss of Wnt11b function...
November 17, 2024 at 4:39 PM
The c-terminal trunkation in the ligand made it look like dominant-negative Wnt11b from Xenopus, where laterality, heart and kidney defects were described before after loss of Wnt11b function...
Miriam and her team found a patient boy with situs inversus, heart and kidney development defects and a frameshift mutation in WNT11.
November 17, 2024 at 4:39 PM
Miriam and her team found a patient boy with situs inversus, heart and kidney development defects and a frameshift mutation in WNT11.
My favorite part of publishing these days.... 🤞
November 15, 2024 at 7:41 AM
My favorite part of publishing these days.... 🤞
Happy holidays and a great start into 2024 from the Walentek Lab!! More cilia, xenopus and mucociliary adventures to come 🐸♥️👊
December 24, 2023 at 10:35 AM
Happy holidays and a great start into 2024 from the Walentek Lab!! More cilia, xenopus and mucociliary adventures to come 🐸♥️👊
Hemingway special time... 🥂
September 16, 2023 at 5:00 PM
Hemingway special time... 🥂