Vladimir Volkov 🇺🇦
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vladimirvolkov.bsky.social
Vladimir Volkov 🇺🇦
@vladimirvolkov.bsky.social
Reader in Biochemistry @QMUL. Interested in #biophysics #cryoET #multivalency. I post preprints.
he/him
volkovlab.com
These passages about what the UK gov is preparing for the HE sector are quite terrifying:
September 5, 2025 at 10:08 AM
Feeling like a proper Londoner on the 1st night of the Proms!
July 19, 2025 at 4:22 PM
In summary, the following properties are essential but not individually sufficient for proper, stable kinetochore-microtubule attachments: MT-binding of Ndc80, Ndc80:Ndc80 binding, MT-binding of Ska, Ska:Ndc80 interaction. We add Ska:Ska interactions to this list, and propose this model:
(end)
July 7, 2025 at 9:39 AM
Despite the fact that this oligomerisation-deficient Ska binds normally to microtubule ends and walls, it fails to support cold-stable attachments in cells, and fails to stabilise microtubules against shortening in vitro (6/7)
July 7, 2025 at 9:39 AM
On dynamic MTs, Ska decorates the lattice non-uniformly, forming brighter coating near the plus-end. Screening a variety of constructs, we found a point mutant that fails to form these bright regions, but binds to MTs and their ends OK. Thus, this mutant lacks Ska:Ska, but not Ska:MT binding (5/7)
July 7, 2025 at 9:39 AM
Low-resolution subtomogram averaging revealed ordered "trains" of Ndc80 along tubulin protofilaments, but now ordered binding of Ska (4/7)
July 7, 2025 at 9:39 AM
Performing cryoET on microtubules stabilised on grids in this way, we found that stabilised microtubule ends carry short and clustered protofilaments - properties that we previously described as characteristic for growing microtubules, despite the fact that microtubules were actually shortening(3/7)
July 7, 2025 at 9:39 AM
How do kinetochores stabilise microtubules against shortening and detachment? We found conditions in which addition of Ndc80 or Ndc80+Ska to dynamic microtubules, simultaneously with dilution of tubulin, prevents microtubules from falling apart. What holds them together? (2/7)
July 7, 2025 at 9:39 AM
I like the sauna song! But my favourite weirdos this year are Tautumeitas from Latvia. Too bad songs like this never win
May 17, 2025 at 9:56 PM
When you plunge grids around Easter time
May 9, 2025 at 5:32 PM
Movie you've watched more than 6 times using gifs.
(Hard mode- no Star Wars, Star Trek, or LOTR)
April 26, 2025 at 8:30 PM
Thanks so much @maxotubule.bsky.social for the seminar today! And very glad to see the board in the office used properly
April 10, 2025 at 6:30 PM
So nice to be back in the cryoET business - the first data acquisition session with a sample from my own lab! ❄️🔬
March 19, 2025 at 11:40 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

Day 19

#booksky
#books
#bookchallenge
December 26, 2024 at 11:45 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

Day 18

#booksky
#books
#bookchallenge
December 25, 2024 at 3:09 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

Day 17

#booksky
#books
#bookchallenge
December 24, 2024 at 11:43 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

Day 16

#booksky
#books
#bookchallenge
December 23, 2024 at 2:24 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

Day 15

#booksky
#books
#bookchallenge
December 22, 2024 at 5:40 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

Day 14

#booksky
#books
#bookchallenge
December 21, 2024 at 4:32 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

Day 13

#booksky
#books
#bookchallenge
December 20, 2024 at 6:20 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

Day 12

#booksky
#books
#bookchallenge
December 19, 2024 at 7:24 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

Day 11

#booksky
#books
#bookchallenge
December 18, 2024 at 7:21 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

Day 10

#booksky
#books
#bookchallenge
December 17, 2024 at 7:18 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

Day 9

#booksky
#books
#bookchallenge
December 16, 2024 at 9:50 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.

Day 8

#booksky
#books
#bookchallenge
December 15, 2024 at 10:43 AM