Vladimir Volkov 🇺🇦
@vladimirvolkov.bsky.social
Reader in Biochemistry @QMUL. Interested in #biophysics #cryoET #multivalency. I post preprints.
he/him
volkovlab.com
he/him
volkovlab.com
These passages about what the UK gov is preparing for the HE sector are quite terrifying:
September 5, 2025 at 10:08 AM
These passages about what the UK gov is preparing for the HE sector are quite terrifying:
Feeling like a proper Londoner on the 1st night of the Proms!
July 19, 2025 at 4:22 PM
Feeling like a proper Londoner on the 1st night of the Proms!
In summary, the following properties are essential but not individually sufficient for proper, stable kinetochore-microtubule attachments: MT-binding of Ndc80, Ndc80:Ndc80 binding, MT-binding of Ska, Ska:Ndc80 interaction. We add Ska:Ska interactions to this list, and propose this model:
(end)
(end)
July 7, 2025 at 9:39 AM
In summary, the following properties are essential but not individually sufficient for proper, stable kinetochore-microtubule attachments: MT-binding of Ndc80, Ndc80:Ndc80 binding, MT-binding of Ska, Ska:Ndc80 interaction. We add Ska:Ska interactions to this list, and propose this model:
(end)
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Despite the fact that this oligomerisation-deficient Ska binds normally to microtubule ends and walls, it fails to support cold-stable attachments in cells, and fails to stabilise microtubules against shortening in vitro (6/7)
July 7, 2025 at 9:39 AM
Despite the fact that this oligomerisation-deficient Ska binds normally to microtubule ends and walls, it fails to support cold-stable attachments in cells, and fails to stabilise microtubules against shortening in vitro (6/7)
On dynamic MTs, Ska decorates the lattice non-uniformly, forming brighter coating near the plus-end. Screening a variety of constructs, we found a point mutant that fails to form these bright regions, but binds to MTs and their ends OK. Thus, this mutant lacks Ska:Ska, but not Ska:MT binding (5/7)
July 7, 2025 at 9:39 AM
On dynamic MTs, Ska decorates the lattice non-uniformly, forming brighter coating near the plus-end. Screening a variety of constructs, we found a point mutant that fails to form these bright regions, but binds to MTs and their ends OK. Thus, this mutant lacks Ska:Ska, but not Ska:MT binding (5/7)
Low-resolution subtomogram averaging revealed ordered "trains" of Ndc80 along tubulin protofilaments, but now ordered binding of Ska (4/7)
July 7, 2025 at 9:39 AM
Low-resolution subtomogram averaging revealed ordered "trains" of Ndc80 along tubulin protofilaments, but now ordered binding of Ska (4/7)
Performing cryoET on microtubules stabilised on grids in this way, we found that stabilised microtubule ends carry short and clustered protofilaments - properties that we previously described as characteristic for growing microtubules, despite the fact that microtubules were actually shortening(3/7)
July 7, 2025 at 9:39 AM
Performing cryoET on microtubules stabilised on grids in this way, we found that stabilised microtubule ends carry short and clustered protofilaments - properties that we previously described as characteristic for growing microtubules, despite the fact that microtubules were actually shortening(3/7)
How do kinetochores stabilise microtubules against shortening and detachment? We found conditions in which addition of Ndc80 or Ndc80+Ska to dynamic microtubules, simultaneously with dilution of tubulin, prevents microtubules from falling apart. What holds them together? (2/7)
July 7, 2025 at 9:39 AM
How do kinetochores stabilise microtubules against shortening and detachment? We found conditions in which addition of Ndc80 or Ndc80+Ska to dynamic microtubules, simultaneously with dilution of tubulin, prevents microtubules from falling apart. What holds them together? (2/7)
I like the sauna song! But my favourite weirdos this year are Tautumeitas from Latvia. Too bad songs like this never win
May 17, 2025 at 9:56 PM
I like the sauna song! But my favourite weirdos this year are Tautumeitas from Latvia. Too bad songs like this never win
When you plunge grids around Easter time
May 9, 2025 at 5:32 PM
When you plunge grids around Easter time
Movie you've watched more than 6 times using gifs.
(Hard mode- no Star Wars, Star Trek, or LOTR)
(Hard mode- no Star Wars, Star Trek, or LOTR)
April 26, 2025 at 8:30 PM
Movie you've watched more than 6 times using gifs.
(Hard mode- no Star Wars, Star Trek, or LOTR)
(Hard mode- no Star Wars, Star Trek, or LOTR)
Thanks so much @maxotubule.bsky.social for the seminar today! And very glad to see the board in the office used properly
April 10, 2025 at 6:30 PM
Thanks so much @maxotubule.bsky.social for the seminar today! And very glad to see the board in the office used properly
So nice to be back in the cryoET business - the first data acquisition session with a sample from my own lab! ❄️🔬
March 19, 2025 at 11:40 AM
So nice to be back in the cryoET business - the first data acquisition session with a sample from my own lab! ❄️🔬
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 19
#booksky
#books
#bookchallenge
Day 19
#booksky
#books
#bookchallenge
December 26, 2024 at 11:45 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 19
#booksky
#books
#bookchallenge
Day 19
#booksky
#books
#bookchallenge
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 18
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#bookchallenge
Day 18
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#bookchallenge
December 25, 2024 at 3:09 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 18
#booksky
#books
#bookchallenge
Day 18
#booksky
#books
#bookchallenge
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 17
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#books
#bookchallenge
Day 17
#booksky
#books
#bookchallenge
December 24, 2024 at 11:43 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 17
#booksky
#books
#bookchallenge
Day 17
#booksky
#books
#bookchallenge
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 16
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#books
#bookchallenge
Day 16
#booksky
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December 23, 2024 at 2:24 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 16
#booksky
#books
#bookchallenge
Day 16
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#bookchallenge
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 15
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#bookchallenge
Day 15
#booksky
#books
#bookchallenge
December 22, 2024 at 5:40 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 15
#booksky
#books
#bookchallenge
Day 15
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#bookchallenge
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 14
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#bookchallenge
Day 14
#booksky
#books
#bookchallenge
December 21, 2024 at 4:32 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 14
#booksky
#books
#bookchallenge
Day 14
#booksky
#books
#bookchallenge
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 13
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#bookchallenge
Day 13
#booksky
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#bookchallenge
December 20, 2024 at 6:20 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 13
#booksky
#books
#bookchallenge
Day 13
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#bookchallenge
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 12
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#bookchallenge
Day 12
#booksky
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#bookchallenge
December 19, 2024 at 7:24 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 12
#booksky
#books
#bookchallenge
Day 12
#booksky
#books
#bookchallenge
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 11
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#bookchallenge
Day 11
#booksky
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#bookchallenge
December 18, 2024 at 7:21 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 11
#booksky
#books
#bookchallenge
Day 11
#booksky
#books
#bookchallenge
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 10
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#bookchallenge
Day 10
#booksky
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#bookchallenge
December 17, 2024 at 7:18 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 10
#booksky
#books
#bookchallenge
Day 10
#booksky
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#bookchallenge
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 9
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#bookchallenge
Day 9
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#bookchallenge
December 16, 2024 at 9:50 PM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 9
#booksky
#books
#bookchallenge
Day 9
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#books
#bookchallenge
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 8
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#bookchallenge
Day 8
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#bookchallenge
December 15, 2024 at 10:43 AM
Choose 20 books that have stayed with you or influenced you. One book per day for 20 days, in no particular order. No explanations, no reviews, just covers.
Day 8
#booksky
#books
#bookchallenge
Day 8
#booksky
#books
#bookchallenge