Aaron and the Hoskins Lab at UW Madison
@uwmadisonrna.bsky.social
Interested in science, RNA, splicing, and single molecule biophysics.
October 30, 2025 at 7:04 PM
Random encounter with miRNA royalty in Houston…it is such a small world!
October 22, 2025 at 9:15 PM
Random encounter with miRNA royalty in Houston…it is such a small world!
For example, we saw ~2000 skipped exon events due to the compound in both cell lines but only ~25% of those were shared. The remaining 75% were unique to either WT or the mutant cells.
October 21, 2025 at 8:43 PM
For example, we saw ~2000 skipped exon events due to the compound in both cell lines but only ~25% of those were shared. The remaining 75% were unique to either WT or the mutant cells.
Finally, Sierra worked with Hai Dang's group at U. Minnesota to show that splicing factor mutant cancer cells (SRSF2-P95H for aficionados) were sensitive to one of these compounds.
October 21, 2025 at 8:43 PM
Finally, Sierra worked with Hai Dang's group at U. Minnesota to show that splicing factor mutant cancer cells (SRSF2-P95H for aficionados) were sensitive to one of these compounds.
From these she found four that not only caused accumulation of the splicing reporters, but actually impacted splicing of an endogenous transcript in strain-specific ways.
October 21, 2025 at 8:43 PM
From these she found four that not only caused accumulation of the splicing reporters, but actually impacted splicing of an endogenous transcript in strain-specific ways.
She then carried out a secondary screen to look at splicing defects more closely using a fluorescent splicing reporter developed by @mannyares.bsky.social .
October 21, 2025 at 8:43 PM
She then carried out a secondary screen to look at splicing defects more closely using a fluorescent splicing reporter developed by @mannyares.bsky.social .
Sierra screened 10s of thousands of compounds and found several hundred that inhibited yeast growth in strain-specific ways.
October 21, 2025 at 8:43 PM
Sierra screened 10s of thousands of compounds and found several hundred that inhibited yeast growth in strain-specific ways.
In this work, Sierra developed a yeast-based screen to look at splicing inhibitors using sensitized yeast strains (that contained "humanized" versions of SF3B1/Hsh155 and Brr2).
October 21, 2025 at 8:43 PM
In this work, Sierra developed a yeast-based screen to look at splicing inhibitors using sensitized yeast strains (that contained "humanized" versions of SF3B1/Hsh155 and Brr2).
How I feel nowadays...
(illustration by Claude)
(illustration by Claude)
October 20, 2025 at 7:37 PM
How I feel nowadays...
(illustration by Claude)
(illustration by Claude)
My pets are insta-famous! See Andor and Sterling at cats_of_madison and Agatha at dogs.of.madison on Instagram :)
October 10, 2025 at 12:05 AM
My pets are insta-famous! See Andor and Sterling at cats_of_madison and Agatha at dogs.of.madison on Instagram :)
Great to catch up with my former undergrad Charlie Schneider and @uwbiochem.bsky.social biochemistry major who is in town today and tomorrow representing Merck for the @uwmadisoncals.bsky.social Biotechnology Student-Employer Connector event!
today.wisc.edu/events/view/...
today.wisc.edu/events/view/...
October 6, 2025 at 5:59 PM
Great to catch up with my former undergrad Charlie Schneider and @uwbiochem.bsky.social biochemistry major who is in town today and tomorrow representing Merck for the @uwmadisoncals.bsky.social Biotechnology Student-Employer Connector event!
today.wisc.edu/events/view/...
today.wisc.edu/events/view/...
Congratulations Bart!
October 2, 2025 at 2:04 PM
Congratulations Bart!
Very excited about next week's RNA Maxigroup seminar from Kristen Lynch @upenn.edu ! Thanks to @rnasociety.bsky.social , @uwbiochem.bsky.social, and the CMB program for sponsoring!
September 29, 2025 at 7:16 PM
Very excited about next week's RNA Maxigroup seminar from Kristen Lynch @upenn.edu ! Thanks to @rnasociety.bsky.social , @uwbiochem.bsky.social, and the CMB program for sponsoring!
I'm participating in the BE 262 course at Caltech this week and we had an amazing field trip to @mtwilsonobs.bsky.social last night!
September 6, 2025 at 6:48 PM
I'm participating in the BE 262 course at Caltech this week and we had an amazing field trip to @mtwilsonobs.bsky.social last night!
A new semester of RNA Maxigroup talks soon begins! Tao Pan visits next Tuesday. Thanks to @uwbiochem.bsky.social, @rnasociety.bsky.social, and UW-Madison CMB for funding.
September 3, 2025 at 4:56 PM
A new semester of RNA Maxigroup talks soon begins! Tao Pan visits next Tuesday. Thanks to @uwbiochem.bsky.social, @rnasociety.bsky.social, and UW-Madison CMB for funding.
August 22, 2025 at 1:01 PM
A CPA (and splicing!) legend and a CPA legend-in-the-making. :) @cshlmeetings.bsky.social #RNAPR25
August 22, 2025 at 12:56 PM
A CPA (and splicing!) legend and a CPA legend-in-the-making. :) @cshlmeetings.bsky.social #RNAPR25
Fantastic talk by Ethan showcasing our work with @lapassmore.bsky.social lab at @cshlmeetings.bsky.social #RNAPR25! Hurray Ethan!
August 20, 2025 at 5:51 PM
Fantastic talk by Ethan showcasing our work with @lapassmore.bsky.social lab at @cshlmeetings.bsky.social #RNAPR25! Hurray Ethan!
Nice email from NEB today!
July 31, 2025 at 3:52 PM
Nice email from NEB today!
Another successful (very hot) summer outing in the books--go karts and a royal feast!
July 30, 2025 at 3:26 PM
Another successful (very hot) summer outing in the books--go karts and a royal feast!
Agatha has two new baby brothers...
July 25, 2025 at 8:56 PM
Agatha has two new baby brothers...
My pet project from the past 4 months is finally coming to fruition… GMO purple tomatoes from www.norfolkhealthyproduce.com
July 22, 2025 at 2:22 PM
My pet project from the past 4 months is finally coming to fruition… GMO purple tomatoes from www.norfolkhealthyproduce.com
Happy 4th of July from Agatha (who also turns 11 today)!!!
July 3, 2025 at 9:11 PM
Happy 4th of July from Agatha (who also turns 11 today)!!!
We propose that the alpha finger is dynamic in response to the 3'SS: folding and unfolding. Only the folded form allows for docking of the Prp22 c-terminal domain.
June 23, 2025 at 7:51 AM
We propose that the alpha finger is dynamic in response to the 3'SS: folding and unfolding. Only the folded form allows for docking of the Prp22 c-terminal domain.