The CRISPR-Cas9 system is used to edit genes. Factors to consider while choosing it are the Protospacer adjacent motif (PAM) site and guide RNA specific to the gene of interest. PAM is 2-6 nts specifically chosen for Cas9 recognition. gRNA + Cas9 bind next to PAM in the target strand and make a cut.

We know the mechanism of Aβ formation (refer image). But the increasing production of Aβ42 over Aβ40 is unknown. As the study suggested, the conformation of the enzymes could play a role. But could there be something else happening? Maybe the mutations in PS1 recruit other enzymes here. #opinion