Thilaga
@thilaga14.bsky.social
M. Tech (Res) Genetic Engineering'22 | Looking for PhD positions in Molecular Biology in Europe
Pinned
Thilaga
@thilaga14.bsky.social
· Jul 3
CRISPR-Cas9 treatment partially restores amyloid-β 42/40 in human fibroblasts with the Alzheimer’s disease PSEN1 M146L mutation
As a novel approach for treatment of early-onset familial Alzheimer’s disease, CRISPR-Cas9-mediated
disruption of PSEN1 in human fibroblasts with the pathogenic PSEN1 M146L mutation
can partially rest...
www.cell.com
I started reading this paper on a new CRISPR-Cas9 treatment for Alzheimer's disease since one of my research interests is neurodegenerative diseases and molecular medicine. I am excited to read and understand the whole paper. and update alongside.
www.cell.com/molecular-th...
www.cell.com/molecular-th...
Another new technique - fluorescence resonance energy transfer/fluorescence lifetime imaging (FRET/FLIM). I've worked on projects studying protein-protein interactions using molecular biology techniques. But this one is definitely advanced to study the same goal. pmc.ncbi.nlm.nih.gov/articles/PMC...
A Guide to Fluorescence Lifetime Microscopy and Förster’s Resonance Energy Transfer in Neuroscience
Fluorescence lifetime microscopy (FLIM) and Förster’s resonance energy transfer (FRET) are advanced optical tools that neuroscientists can employ to interrogate the structure and function of complex b...
pmc.ncbi.nlm.nih.gov
July 26, 2025 at 10:55 AM
Another new technique - fluorescence resonance energy transfer/fluorescence lifetime imaging (FRET/FLIM). I've worked on projects studying protein-protein interactions using molecular biology techniques. But this one is definitely advanced to study the same goal. pmc.ncbi.nlm.nih.gov/articles/PMC...
CRISPResso2 is a data analysis tool developed for analyzing the results of based edited samples using sequencing data.
To know more: pmc.ncbi.nlm.nih.gov/articles/PMC...
To know more: pmc.ncbi.nlm.nih.gov/articles/PMC...
July 23, 2025 at 1:43 PM
CRISPResso2 is a data analysis tool developed for analyzing the results of based edited samples using sequencing data.
To know more: pmc.ncbi.nlm.nih.gov/articles/PMC...
To know more: pmc.ncbi.nlm.nih.gov/articles/PMC...
Reading today's part of the article, I realized while observing the results it is important to make note of each detail to make an informed interpretation. In research every detail, including the unexpected outcomes are important because it's either due to experimental mistake or a new finding.
July 22, 2025 at 2:50 PM
Reading today's part of the article, I realized while observing the results it is important to make note of each detail to make an informed interpretation. In research every detail, including the unexpected outcomes are important because it's either due to experimental mistake or a new finding.
In CRISPR-Cas9, precise recognition and cleavage of the target DNA are extremely important for accurate editing since small changes in DNA cause big consequences. So, selecting the right Cas enzyme, PAM site, and designing guide RNA is important.
July 20, 2025 at 2:05 PM
In CRISPR-Cas9, precise recognition and cleavage of the target DNA are extremely important for accurate editing since small changes in DNA cause big consequences. So, selecting the right Cas enzyme, PAM site, and designing guide RNA is important.
I'm done with overall study of the current paper I'm reviewing. Now I'm going to focus on the methodology before going to results. Right now I'm interested in understanding different techniques and data analysis.
July 18, 2025 at 3:53 PM
I'm done with overall study of the current paper I'm reviewing. Now I'm going to focus on the methodology before going to results. Right now I'm interested in understanding different techniques and data analysis.
Why is early-onset AD an ideal candidate for gene editing strategies? The study says it's because of autosomal dominant genes. But I don't how and why. I'll find the answer and tell tomorrow.
July 14, 2025 at 3:25 PM
Why is early-onset AD an ideal candidate for gene editing strategies? The study says it's because of autosomal dominant genes. But I don't how and why. I'll find the answer and tell tomorrow.
Today I learned, more like recalled, the importance of basic research before translation. In the current papar, I learned γ-secretase binds to many substrates, possible reason for adverse effects by its inhibitors in phase 3 clinical trials. Now I'm interested to find the pathways it's involved in.
July 12, 2025 at 2:29 PM
Today I learned, more like recalled, the importance of basic research before translation. In the current papar, I learned γ-secretase binds to many substrates, possible reason for adverse effects by its inhibitors in phase 3 clinical trials. Now I'm interested to find the pathways it's involved in.
Reading the discussion section of the current paper. I need to focus more on the work to understand it. So no post on the paper today.
July 11, 2025 at 2:30 PM
Reading the discussion section of the current paper. I need to focus more on the work to understand it. So no post on the paper today.
I started reading this paper on a new CRISPR-Cas9 treatment for Alzheimer's disease since one of my research interests is neurodegenerative diseases and molecular medicine. I am excited to read and understand the whole paper. and update alongside.
www.cell.com/molecular-th...
www.cell.com/molecular-th...
CRISPR-Cas9 treatment partially restores amyloid-β 42/40 in human fibroblasts with the Alzheimer’s disease PSEN1 M146L mutation
As a novel approach for treatment of early-onset familial Alzheimer’s disease, CRISPR-Cas9-mediated
disruption of PSEN1 in human fibroblasts with the pathogenic PSEN1 M146L mutation
can partially rest...
www.cell.com
July 3, 2025 at 6:33 PM
I started reading this paper on a new CRISPR-Cas9 treatment for Alzheimer's disease since one of my research interests is neurodegenerative diseases and molecular medicine. I am excited to read and understand the whole paper. and update alongside.
www.cell.com/molecular-th...
www.cell.com/molecular-th...
In the upcoming days, I am going to review and update on scientific papers I am going to read on molecular medicine, and other interesting papers I might like. So, stay tuned, guys!
July 3, 2025 at 6:27 PM
In the upcoming days, I am going to review and update on scientific papers I am going to read on molecular medicine, and other interesting papers I might like. So, stay tuned, guys!