Bhavesh Parmar
@thatmassspecguy.bsky.social
High-throughput proteomics and low-throughput SM-banter.
Thank you all the colleagues that reached out in person and via DMs to share their thoughts, organisers for giving me the opportunity and amazing chair Martha Ingola and @mtrost.bsky.social
June 18, 2025 at 11:47 AM
Thank you all the colleagues that reached out in person and via DMs to share their thoughts, organisers for giving me the opportunity and amazing chair Martha Ingola and @mtrost.bsky.social
🤷♂️ the company rep says you can reuse them and we’ve had a good experience working with 100ng-35ug. But perhaps making your own tips might be cheaper.
March 24, 2025 at 2:16 PM
🤷♂️ the company rep says you can reuse them and we’ve had a good experience working with 100ng-35ug. But perhaps making your own tips might be cheaper.
During Masters thesis under Prof. Paul Haynes and Prof. Mark Molloy (Macquarie University) circa 2012-14, and a lot more as a technician at a core facility 2014-17 with @itsbinir.bsky.social hanging around.
March 20, 2025 at 1:35 PM
During Masters thesis under Prof. Paul Haynes and Prof. Mark Molloy (Macquarie University) circa 2012-14, and a lot more as a technician at a core facility 2014-17 with @itsbinir.bsky.social hanging around.
Not sure, but we usually get >90% 0 missed cleavage with STrap and SP3 at 47C, 60 mins in 50mM TEAB/EPPS.
March 18, 2025 at 6:51 PM
Not sure, but we usually get >90% 0 missed cleavage with STrap and SP3 at 47C, 60 mins in 50mM TEAB/EPPS.
Congrats Tina!
February 10, 2025 at 7:25 PM
Congrats Tina!
Trying to find who is, and how. But I’m afraid many tribrids and QEs will need to be retired and replaced with TOFs if labs/core facilities are to keep up.
January 8, 2025 at 12:10 AM
Trying to find who is, and how. But I’m afraid many tribrids and QEs will need to be retired and replaced with TOFs if labs/core facilities are to keep up.
Any specific reason for OT-OT instead of OT-IT?
January 7, 2025 at 5:34 PM
Any specific reason for OT-OT instead of OT-IT?
Haven’t tried DIA on lumos but I’m inclined to believe 2hrs/sample with FAIMS-OT-IT might actually be faster for the same quant ids.
January 7, 2025 at 2:33 PM
Haven’t tried DIA on lumos but I’m inclined to believe 2hrs/sample with FAIMS-OT-IT might actually be faster for the same quant ids.
Similar for us on ascend but for lumos which doesn’t support RTS, SPS-MS3 with 24 TMT18 fractions ends up being 2 days for about 8k+ quant. With large batches and IRS channel, the throughput feels very 2018(?).
January 7, 2025 at 2:33 PM
Similar for us on ascend but for lumos which doesn’t support RTS, SPS-MS3 with 24 TMT18 fractions ends up being 2 days for about 8k+ quant. With large batches and IRS channel, the throughput feels very 2018(?).
‘30spd’ == anyone getting TMT16 or TMT32 fractions finished within a day with >8k proteins?
January 6, 2025 at 5:54 PM
‘30spd’ == anyone getting TMT16 or TMT32 fractions finished within a day with >8k proteins?